Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine

The stable and highly catalytic Fe metal–organic framework (FeMOF) nanosol was prepared and characterized by electron microscopy, and energy and molecular spectral analysis. It was found that FeMOF strongly catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) by H(2)O(2) to produce TMBox,...

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Autores principales: Zhao, Yuxiang, Chen, Qianmiao, Zhang, Chi, Li, Chongning, Jiang, Zhiliang, Liang, Aihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688084/
https://www.ncbi.nlm.nih.gov/pubmed/36354430
http://dx.doi.org/10.3390/bios12110920
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author Zhao, Yuxiang
Chen, Qianmiao
Zhang, Chi
Li, Chongning
Jiang, Zhiliang
Liang, Aihui
author_facet Zhao, Yuxiang
Chen, Qianmiao
Zhang, Chi
Li, Chongning
Jiang, Zhiliang
Liang, Aihui
author_sort Zhao, Yuxiang
collection PubMed
description The stable and highly catalytic Fe metal–organic framework (FeMOF) nanosol was prepared and characterized by electron microscopy, and energy and molecular spectral analysis. It was found that FeMOF strongly catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) by H(2)O(2) to produce TMBox, which had a fluorescence (FL) peak at 410 nm. When silver nanoparticles were added, it exhibited strong resonance Rayleigh scattering (RRS) activity and surface-enhanced Raman scattering (SERS) effect. This new FeMOF nanocatalytic trimode indicator reaction was combined with the glyphosate aptamer reaction to establish a new SERS/RRS/FL trimode biosensor for glyphosate. The sensor can be used for the analysis of environmental wastewater, and a new method for detecting glyphosate content in wastewater is proposed. The linear range of the sensor is 0.1–14 nmol/L, the detection limit is 0.05 nmol/L, the recovery is 92.1–97.5%, and the relative standard deviation is 3.6–8.7%.
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spelling pubmed-96880842022-11-25 Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine Zhao, Yuxiang Chen, Qianmiao Zhang, Chi Li, Chongning Jiang, Zhiliang Liang, Aihui Biosensors (Basel) Article The stable and highly catalytic Fe metal–organic framework (FeMOF) nanosol was prepared and characterized by electron microscopy, and energy and molecular spectral analysis. It was found that FeMOF strongly catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) by H(2)O(2) to produce TMBox, which had a fluorescence (FL) peak at 410 nm. When silver nanoparticles were added, it exhibited strong resonance Rayleigh scattering (RRS) activity and surface-enhanced Raman scattering (SERS) effect. This new FeMOF nanocatalytic trimode indicator reaction was combined with the glyphosate aptamer reaction to establish a new SERS/RRS/FL trimode biosensor for glyphosate. The sensor can be used for the analysis of environmental wastewater, and a new method for detecting glyphosate content in wastewater is proposed. The linear range of the sensor is 0.1–14 nmol/L, the detection limit is 0.05 nmol/L, the recovery is 92.1–97.5%, and the relative standard deviation is 3.6–8.7%. MDPI 2022-10-25 /pmc/articles/PMC9688084/ /pubmed/36354430 http://dx.doi.org/10.3390/bios12110920 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Yuxiang
Chen, Qianmiao
Zhang, Chi
Li, Chongning
Jiang, Zhiliang
Liang, Aihui
Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine
title Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine
title_full Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine
title_fullStr Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine
title_full_unstemmed Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine
title_short Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine
title_sort aptamer trimode biosensor for trace glyphosate based on femof catalytic oxidation of tetramethylbenzidine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688084/
https://www.ncbi.nlm.nih.gov/pubmed/36354430
http://dx.doi.org/10.3390/bios12110920
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