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A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase

Enzyme detection in liquid samples is a complex laboratory procedure, based on assays that are generally time- and cost-consuming, and require specialized personnel. Surface acoustic wave sensors can be used for this application, overcoming the cited limitations. To give our contribution, in this wo...

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Autores principales: Gagliardi, Mariacristina, Agostini, Matteo, Lunardelli, Francesco, Miranda, Alessio, Luminare, Antonella Giuliana, Cervelli, Fabrizio, Gambineri, Francesca, Cecchini, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688093/
https://www.ncbi.nlm.nih.gov/pubmed/36421128
http://dx.doi.org/10.3390/bios12111010
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author Gagliardi, Mariacristina
Agostini, Matteo
Lunardelli, Francesco
Miranda, Alessio
Luminare, Antonella Giuliana
Cervelli, Fabrizio
Gambineri, Francesca
Cecchini, Marco
author_facet Gagliardi, Mariacristina
Agostini, Matteo
Lunardelli, Francesco
Miranda, Alessio
Luminare, Antonella Giuliana
Cervelli, Fabrizio
Gambineri, Francesca
Cecchini, Marco
author_sort Gagliardi, Mariacristina
collection PubMed
description Enzyme detection in liquid samples is a complex laboratory procedure, based on assays that are generally time- and cost-consuming, and require specialized personnel. Surface acoustic wave sensors can be used for this application, overcoming the cited limitations. To give our contribution, in this work we present the bottom-up development of a surface acoustic wave biosensor to detect active α-glycosidase in aqueous solutions. Our device, optimized to work at an ultra-high frequency (around 740 MHz), is functionalized with a newly synthesized probe 7-mercapto-1-eptyl-D-maltoside, bringing one maltoside terminal moiety. The probe is designed ad hoc for this application and tested in-cuvette to analyze the enzymatic conversion kinetics at different times, temperatures and enzyme concentrations. Preliminary data are used to optimize the detection protocol with the SAW device. In around 60 min, the SAW device is able to detect the enzymatic conversion of the maltoside unit into glucose in the presence of the active enzyme. We obtained successful α-glycosidase detection in the concentration range 0.15–150 U/mL, with an increasing signal in the range up to 15 U/mL. We also checked the sensor performance in the presence of an enzyme inhibitor as a control test, with a signal decrease of 80% in the presence of the inhibitor. The results demonstrate the synergic effect of our SAW Lab-on-a-Chip and probe design as a valid alternative to conventional laboratory tests.
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spelling pubmed-96880932022-11-25 A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase Gagliardi, Mariacristina Agostini, Matteo Lunardelli, Francesco Miranda, Alessio Luminare, Antonella Giuliana Cervelli, Fabrizio Gambineri, Francesca Cecchini, Marco Biosensors (Basel) Article Enzyme detection in liquid samples is a complex laboratory procedure, based on assays that are generally time- and cost-consuming, and require specialized personnel. Surface acoustic wave sensors can be used for this application, overcoming the cited limitations. To give our contribution, in this work we present the bottom-up development of a surface acoustic wave biosensor to detect active α-glycosidase in aqueous solutions. Our device, optimized to work at an ultra-high frequency (around 740 MHz), is functionalized with a newly synthesized probe 7-mercapto-1-eptyl-D-maltoside, bringing one maltoside terminal moiety. The probe is designed ad hoc for this application and tested in-cuvette to analyze the enzymatic conversion kinetics at different times, temperatures and enzyme concentrations. Preliminary data are used to optimize the detection protocol with the SAW device. In around 60 min, the SAW device is able to detect the enzymatic conversion of the maltoside unit into glucose in the presence of the active enzyme. We obtained successful α-glycosidase detection in the concentration range 0.15–150 U/mL, with an increasing signal in the range up to 15 U/mL. We also checked the sensor performance in the presence of an enzyme inhibitor as a control test, with a signal decrease of 80% in the presence of the inhibitor. The results demonstrate the synergic effect of our SAW Lab-on-a-Chip and probe design as a valid alternative to conventional laboratory tests. MDPI 2022-11-11 /pmc/articles/PMC9688093/ /pubmed/36421128 http://dx.doi.org/10.3390/bios12111010 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gagliardi, Mariacristina
Agostini, Matteo
Lunardelli, Francesco
Miranda, Alessio
Luminare, Antonella Giuliana
Cervelli, Fabrizio
Gambineri, Francesca
Cecchini, Marco
A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase
title A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase
title_full A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase
title_fullStr A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase
title_full_unstemmed A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase
title_short A Surface Acoustic Wave (SAW)-Based Lab-on-Chip for the Detection of Active α-Glycosidase
title_sort surface acoustic wave (saw)-based lab-on-chip for the detection of active α-glycosidase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688093/
https://www.ncbi.nlm.nih.gov/pubmed/36421128
http://dx.doi.org/10.3390/bios12111010
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