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Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells

During disease progression and bone metastasis, breast tumor cells interact with various types of bystander cells residing in the tumor microenvironment. Such interactions prompt tumor cell heterogeneity. We used successive co-culture as an experimental model to examine cancer–bystander cell interac...

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Autores principales: Wang, Ruoxiang, Wang, Xudong, Yin, Liyuan, Yin, Lijuan, Chu, Gina Chia-Yi, Hu, Peizhen, Ou, Yan, Zhang, Yi, Lewis, Michael S., Pandol, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688235/
https://www.ncbi.nlm.nih.gov/pubmed/36428982
http://dx.doi.org/10.3390/cells11223553
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author Wang, Ruoxiang
Wang, Xudong
Yin, Liyuan
Yin, Lijuan
Chu, Gina Chia-Yi
Hu, Peizhen
Ou, Yan
Zhang, Yi
Lewis, Michael S.
Pandol, Stephen J.
author_facet Wang, Ruoxiang
Wang, Xudong
Yin, Liyuan
Yin, Lijuan
Chu, Gina Chia-Yi
Hu, Peizhen
Ou, Yan
Zhang, Yi
Lewis, Michael S.
Pandol, Stephen J.
author_sort Wang, Ruoxiang
collection PubMed
description During disease progression and bone metastasis, breast tumor cells interact with various types of bystander cells residing in the tumor microenvironment. Such interactions prompt tumor cell heterogeneity. We used successive co-culture as an experimental model to examine cancer–bystander cell interaction. RMCF7-2, a clone of the human breast cancer MCF-7 cells tagged with a red fluorescent protein, was tracked for morphologic, behavioral, and gene expression changes. Co-cultured with various types of hematopoietic cells, RMCF7-2 adopted stable changes to a rounded shape in suspension growth of red fluorescent cells, from which derivative clones displayed marked expressional changes of marker proteins, including reduced E-cadherin and estrogen receptor α, and loss of progesterone receptor. In a successive co-culture with bone marrow-derived mesenchymal stem/stromal cells, the red fluorescent clones in suspension growth changed once more, adopting an attachment growth, but in diversified shapes. Red fluorescent clones recovered from the second-round co-culture were heterogeneous in morphology, but retained the altered marker protein expression while displaying increased proliferation, migration, and xenograft tumor formation. Interaction with bystander cells caused permanent morphologic, growth behavioral, and gene expressional changes under successive co-culture, which is a powerful model for studying cancer cell heterogeneity during breast cancer progression and metastasis.
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spelling pubmed-96882352022-11-25 Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells Wang, Ruoxiang Wang, Xudong Yin, Liyuan Yin, Lijuan Chu, Gina Chia-Yi Hu, Peizhen Ou, Yan Zhang, Yi Lewis, Michael S. Pandol, Stephen J. Cells Article During disease progression and bone metastasis, breast tumor cells interact with various types of bystander cells residing in the tumor microenvironment. Such interactions prompt tumor cell heterogeneity. We used successive co-culture as an experimental model to examine cancer–bystander cell interaction. RMCF7-2, a clone of the human breast cancer MCF-7 cells tagged with a red fluorescent protein, was tracked for morphologic, behavioral, and gene expression changes. Co-cultured with various types of hematopoietic cells, RMCF7-2 adopted stable changes to a rounded shape in suspension growth of red fluorescent cells, from which derivative clones displayed marked expressional changes of marker proteins, including reduced E-cadherin and estrogen receptor α, and loss of progesterone receptor. In a successive co-culture with bone marrow-derived mesenchymal stem/stromal cells, the red fluorescent clones in suspension growth changed once more, adopting an attachment growth, but in diversified shapes. Red fluorescent clones recovered from the second-round co-culture were heterogeneous in morphology, but retained the altered marker protein expression while displaying increased proliferation, migration, and xenograft tumor formation. Interaction with bystander cells caused permanent morphologic, growth behavioral, and gene expressional changes under successive co-culture, which is a powerful model for studying cancer cell heterogeneity during breast cancer progression and metastasis. MDPI 2022-11-10 /pmc/articles/PMC9688235/ /pubmed/36428982 http://dx.doi.org/10.3390/cells11223553 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Ruoxiang
Wang, Xudong
Yin, Liyuan
Yin, Lijuan
Chu, Gina Chia-Yi
Hu, Peizhen
Ou, Yan
Zhang, Yi
Lewis, Michael S.
Pandol, Stephen J.
Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells
title Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells
title_full Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells
title_fullStr Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells
title_full_unstemmed Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells
title_short Breast Cancer MCF-7 Cells Acquire Heterogeneity during Successive Co-Culture with Hematopoietic and Bone Marrow-Derived Mesenchymal Stem/Stromal Cells
title_sort breast cancer mcf-7 cells acquire heterogeneity during successive co-culture with hematopoietic and bone marrow-derived mesenchymal stem/stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688235/
https://www.ncbi.nlm.nih.gov/pubmed/36428982
http://dx.doi.org/10.3390/cells11223553
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