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Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein

Currently, significant progress is being made in the prevention, treatment and prognosis of many types of cancer, using biological markers to assess current physiological processes in the body, including risk assessment, differential diagnosis, screening, treatment determination and monitoring of di...

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Autores principales: Paltusheva, Zhaniya U., Ashikbayeva, Zhannat, Tosi, Daniele, Gritsenko, Lesya V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688241/
https://www.ncbi.nlm.nih.gov/pubmed/36421133
http://dx.doi.org/10.3390/bios12111015
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author Paltusheva, Zhaniya U.
Ashikbayeva, Zhannat
Tosi, Daniele
Gritsenko, Lesya V.
author_facet Paltusheva, Zhaniya U.
Ashikbayeva, Zhannat
Tosi, Daniele
Gritsenko, Lesya V.
author_sort Paltusheva, Zhaniya U.
collection PubMed
description Currently, significant progress is being made in the prevention, treatment and prognosis of many types of cancer, using biological markers to assess current physiological processes in the body, including risk assessment, differential diagnosis, screening, treatment determination and monitoring of disease progression. The interaction of protein coding gene CD44 with the corresponding ligands promotes the processes of invasion and migration in metastases. The study of new and rapid methods for the quantitative determination of the CD44 protein is essential for timely diagnosis and therapy. Current methods for detecting this protein use labeled assay reagents and are time consuming. In this paper, a fiber-optic biosensor with a spherical tip coated with a thin layer of zinc oxide (ZnO) with a thickness of 100 nm, deposited using a low-cost sol–gel method, is developed to measure the CD44 protein in the range from 100 aM to 100 nM. This sensor is easy to manufacture, has a good response to the protein change with detection limit of 0.8 fM, and has high sensitivity to the changes in the refractive index (RI) of the environment. In addition, this work demonstrates the possibility of achieving sensor regeneration without damage to the functionalized surface. The sensitivity of the obtained sensor was tested in relation to the concentration of the control protein, as well as without antibodies—CD44.
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spelling pubmed-96882412022-11-25 Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein Paltusheva, Zhaniya U. Ashikbayeva, Zhannat Tosi, Daniele Gritsenko, Lesya V. Biosensors (Basel) Article Currently, significant progress is being made in the prevention, treatment and prognosis of many types of cancer, using biological markers to assess current physiological processes in the body, including risk assessment, differential diagnosis, screening, treatment determination and monitoring of disease progression. The interaction of protein coding gene CD44 with the corresponding ligands promotes the processes of invasion and migration in metastases. The study of new and rapid methods for the quantitative determination of the CD44 protein is essential for timely diagnosis and therapy. Current methods for detecting this protein use labeled assay reagents and are time consuming. In this paper, a fiber-optic biosensor with a spherical tip coated with a thin layer of zinc oxide (ZnO) with a thickness of 100 nm, deposited using a low-cost sol–gel method, is developed to measure the CD44 protein in the range from 100 aM to 100 nM. This sensor is easy to manufacture, has a good response to the protein change with detection limit of 0.8 fM, and has high sensitivity to the changes in the refractive index (RI) of the environment. In addition, this work demonstrates the possibility of achieving sensor regeneration without damage to the functionalized surface. The sensitivity of the obtained sensor was tested in relation to the concentration of the control protein, as well as without antibodies—CD44. MDPI 2022-11-14 /pmc/articles/PMC9688241/ /pubmed/36421133 http://dx.doi.org/10.3390/bios12111015 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paltusheva, Zhaniya U.
Ashikbayeva, Zhannat
Tosi, Daniele
Gritsenko, Lesya V.
Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein
title Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein
title_full Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein
title_fullStr Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein
title_full_unstemmed Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein
title_short Highly Sensitive Zinc Oxide Fiber-Optic Biosensor for the Detection of CD44 Protein
title_sort highly sensitive zinc oxide fiber-optic biosensor for the detection of cd44 protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688241/
https://www.ncbi.nlm.nih.gov/pubmed/36421133
http://dx.doi.org/10.3390/bios12111015
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