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Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China

Rickettsiales (Rickettsia spp., Ehrlichia spp., and Anaplasma spp., etc.) are generally recognized as potentially emerging tick-borne pathogens. However, some bacteria and areas in China remain uninvestigated. In this study, we collected 113 ticks from mammals in Guizhou Province, Southwest China, a...

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Autores principales: Lu, Miao, Meng, Chao, Zhang, Bing, Wang, Xiao, Tian, Junhua, Tang, Guangpeng, Wang, Wen, Li, Na, Li, Mengyao, Xu, Xiaoyu, Sun, Yue, Duan, Chengyu, Qin, Xincheng, Li, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688252/
https://www.ncbi.nlm.nih.gov/pubmed/36421715
http://dx.doi.org/10.3390/biom12111701
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author Lu, Miao
Meng, Chao
Zhang, Bing
Wang, Xiao
Tian, Junhua
Tang, Guangpeng
Wang, Wen
Li, Na
Li, Mengyao
Xu, Xiaoyu
Sun, Yue
Duan, Chengyu
Qin, Xincheng
Li, Kun
author_facet Lu, Miao
Meng, Chao
Zhang, Bing
Wang, Xiao
Tian, Junhua
Tang, Guangpeng
Wang, Wen
Li, Na
Li, Mengyao
Xu, Xiaoyu
Sun, Yue
Duan, Chengyu
Qin, Xincheng
Li, Kun
author_sort Lu, Miao
collection PubMed
description Rickettsiales (Rickettsia spp., Ehrlichia spp., and Anaplasma spp., etc.) are generally recognized as potentially emerging tick-borne pathogens. However, some bacteria and areas in China remain uninvestigated. In this study, we collected 113 ticks from mammals in Guizhou Province, Southwest China, and screened for the Rickettsiales bacteria. Subsequently, two spotted fever group Rickettsia species and one Candidatus Lariskella sp. were detected and characterized. “Candidatus Rickettsia jingxinensis” was detected in Rhipicephalus microplus (1/1), Haemaphysalis flava (1/3, 33.33%), Haemaphysalis kitaokai (1/3), and Ixodes sinensis (4/101, 3.96%), whereas Rickettsia monacensis was positive in H. flava (1/3), H. kitaokai (2/3), and I. sinensis ticks (74/101, 73.27%). At least two variants/sub-genotypes were identified in the R. monacensis isolates, and the strikingly high prevalence of R. monacensis may suggest a risk of human infection. Unexpectedly, a Candidatus Lariskella sp. belonging to the family Candidatus Midichloriaceae was detected from Ixodes ovatus (1/4) and I. sinensis (10/101, 9.90%). The gltA and groEL gene sequences were successfully obtained, and they show the highest (74.63–74.89% and 73.31%) similarities to “Candidatus Midichloria mitochondrii”, respectively. Herein, we name the species “Candidatus Lariskella guizhouensis”. These may be the first recovered gltA and groEL sequences of the genus Candidatus Lariskella.
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spelling pubmed-96882522022-11-25 Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China Lu, Miao Meng, Chao Zhang, Bing Wang, Xiao Tian, Junhua Tang, Guangpeng Wang, Wen Li, Na Li, Mengyao Xu, Xiaoyu Sun, Yue Duan, Chengyu Qin, Xincheng Li, Kun Biomolecules Article Rickettsiales (Rickettsia spp., Ehrlichia spp., and Anaplasma spp., etc.) are generally recognized as potentially emerging tick-borne pathogens. However, some bacteria and areas in China remain uninvestigated. In this study, we collected 113 ticks from mammals in Guizhou Province, Southwest China, and screened for the Rickettsiales bacteria. Subsequently, two spotted fever group Rickettsia species and one Candidatus Lariskella sp. were detected and characterized. “Candidatus Rickettsia jingxinensis” was detected in Rhipicephalus microplus (1/1), Haemaphysalis flava (1/3, 33.33%), Haemaphysalis kitaokai (1/3), and Ixodes sinensis (4/101, 3.96%), whereas Rickettsia monacensis was positive in H. flava (1/3), H. kitaokai (2/3), and I. sinensis ticks (74/101, 73.27%). At least two variants/sub-genotypes were identified in the R. monacensis isolates, and the strikingly high prevalence of R. monacensis may suggest a risk of human infection. Unexpectedly, a Candidatus Lariskella sp. belonging to the family Candidatus Midichloriaceae was detected from Ixodes ovatus (1/4) and I. sinensis (10/101, 9.90%). The gltA and groEL gene sequences were successfully obtained, and they show the highest (74.63–74.89% and 73.31%) similarities to “Candidatus Midichloria mitochondrii”, respectively. Herein, we name the species “Candidatus Lariskella guizhouensis”. These may be the first recovered gltA and groEL sequences of the genus Candidatus Lariskella. MDPI 2022-11-17 /pmc/articles/PMC9688252/ /pubmed/36421715 http://dx.doi.org/10.3390/biom12111701 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Miao
Meng, Chao
Zhang, Bing
Wang, Xiao
Tian, Junhua
Tang, Guangpeng
Wang, Wen
Li, Na
Li, Mengyao
Xu, Xiaoyu
Sun, Yue
Duan, Chengyu
Qin, Xincheng
Li, Kun
Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China
title Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China
title_full Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China
title_fullStr Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China
title_full_unstemmed Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China
title_short Prevalence of Spotted Fever Group Rickettsia and Candidatus Lariskella in Multiple Tick Species from Guizhou Province, China
title_sort prevalence of spotted fever group rickettsia and candidatus lariskella in multiple tick species from guizhou province, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688252/
https://www.ncbi.nlm.nih.gov/pubmed/36421715
http://dx.doi.org/10.3390/biom12111701
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