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JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
The histone demethylase JMJD1C is associated with human platelet counts. The JMJD1C knockout in zebrafish and mice leads to the ablation of megakaryocyte–erythroid lineage anemia. However, the specific expression, function, and mechanism of JMJD1C in megakaryopoiesis remain unknown. Here, we used ce...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688414/ https://www.ncbi.nlm.nih.gov/pubmed/36429088 http://dx.doi.org/10.3390/cells11223660 |
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author | Wang, Jialing Liu, Xiaodan Wang, Haixia Qin, Lili Feng, Anhua Qi, Daoxin Wang, Haihua Zhao, Yao Kong, Lihua Wang, Haiying Wang, Lin Hu, Zhenbo Xu, Xin |
author_facet | Wang, Jialing Liu, Xiaodan Wang, Haixia Qin, Lili Feng, Anhua Qi, Daoxin Wang, Haihua Zhao, Yao Kong, Lihua Wang, Haiying Wang, Lin Hu, Zhenbo Xu, Xin |
author_sort | Wang, Jialing |
collection | PubMed |
description | The histone demethylase JMJD1C is associated with human platelet counts. The JMJD1C knockout in zebrafish and mice leads to the ablation of megakaryocyte–erythroid lineage anemia. However, the specific expression, function, and mechanism of JMJD1C in megakaryopoiesis remain unknown. Here, we used cell line models, cord blood cells, and thrombocytopenia samples, to detect the JMJD1C expression. ShRNA of JMJD1C and a specific peptide agonist of JMJD1C, SAH-JZ3, were used to explore the JMJD1C function in the cell line models. The actin ratio in megakaryopoiesis for the JMJDC modulation was also measured. Mass spectrometry was used to identify the JMJD1C-interacting proteins. We first show the JMJD1C expression difference in the PMA-induced cell line models, the thrombopoietin (TPO)-induced megakaryocyte differentiation of the cord blood cells, and also the thrombocytopenia patients, compared to the normal controls. The ShRNA of JMJD1C and SAH-JZ3 showed different effects, which were consistent with the expression of JMJD1C in the cell line models. The effort to find the underlying mechanism of JMJD1C in megakaryopoiesis, led to the discovery that SAH-JZ3 decreases F-actin in K562 cells and increases F-actin in MEG-01 cells. We further performed mass spectrometry to identify the potential JMJD1C-interacting proteins and found that the important Ran GTPase interacts with JMJD1C. To sum up, JMJD1C probably regulates megakaryopoiesis by influencing the actin network. |
format | Online Article Text |
id | pubmed-9688414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96884142022-11-25 JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network Wang, Jialing Liu, Xiaodan Wang, Haixia Qin, Lili Feng, Anhua Qi, Daoxin Wang, Haihua Zhao, Yao Kong, Lihua Wang, Haiying Wang, Lin Hu, Zhenbo Xu, Xin Cells Article The histone demethylase JMJD1C is associated with human platelet counts. The JMJD1C knockout in zebrafish and mice leads to the ablation of megakaryocyte–erythroid lineage anemia. However, the specific expression, function, and mechanism of JMJD1C in megakaryopoiesis remain unknown. Here, we used cell line models, cord blood cells, and thrombocytopenia samples, to detect the JMJD1C expression. ShRNA of JMJD1C and a specific peptide agonist of JMJD1C, SAH-JZ3, were used to explore the JMJD1C function in the cell line models. The actin ratio in megakaryopoiesis for the JMJDC modulation was also measured. Mass spectrometry was used to identify the JMJD1C-interacting proteins. We first show the JMJD1C expression difference in the PMA-induced cell line models, the thrombopoietin (TPO)-induced megakaryocyte differentiation of the cord blood cells, and also the thrombocytopenia patients, compared to the normal controls. The ShRNA of JMJD1C and SAH-JZ3 showed different effects, which were consistent with the expression of JMJD1C in the cell line models. The effort to find the underlying mechanism of JMJD1C in megakaryopoiesis, led to the discovery that SAH-JZ3 decreases F-actin in K562 cells and increases F-actin in MEG-01 cells. We further performed mass spectrometry to identify the potential JMJD1C-interacting proteins and found that the important Ran GTPase interacts with JMJD1C. To sum up, JMJD1C probably regulates megakaryopoiesis by influencing the actin network. MDPI 2022-11-18 /pmc/articles/PMC9688414/ /pubmed/36429088 http://dx.doi.org/10.3390/cells11223660 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Jialing Liu, Xiaodan Wang, Haixia Qin, Lili Feng, Anhua Qi, Daoxin Wang, Haihua Zhao, Yao Kong, Lihua Wang, Haiying Wang, Lin Hu, Zhenbo Xu, Xin JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network |
title | JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network |
title_full | JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network |
title_fullStr | JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network |
title_full_unstemmed | JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network |
title_short | JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network |
title_sort | jmjd1c regulates megakaryopoiesis in in vitro models through the actin network |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688414/ https://www.ncbi.nlm.nih.gov/pubmed/36429088 http://dx.doi.org/10.3390/cells11223660 |
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