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JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network

The histone demethylase JMJD1C is associated with human platelet counts. The JMJD1C knockout in zebrafish and mice leads to the ablation of megakaryocyte–erythroid lineage anemia. However, the specific expression, function, and mechanism of JMJD1C in megakaryopoiesis remain unknown. Here, we used ce...

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Autores principales: Wang, Jialing, Liu, Xiaodan, Wang, Haixia, Qin, Lili, Feng, Anhua, Qi, Daoxin, Wang, Haihua, Zhao, Yao, Kong, Lihua, Wang, Haiying, Wang, Lin, Hu, Zhenbo, Xu, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688414/
https://www.ncbi.nlm.nih.gov/pubmed/36429088
http://dx.doi.org/10.3390/cells11223660
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author Wang, Jialing
Liu, Xiaodan
Wang, Haixia
Qin, Lili
Feng, Anhua
Qi, Daoxin
Wang, Haihua
Zhao, Yao
Kong, Lihua
Wang, Haiying
Wang, Lin
Hu, Zhenbo
Xu, Xin
author_facet Wang, Jialing
Liu, Xiaodan
Wang, Haixia
Qin, Lili
Feng, Anhua
Qi, Daoxin
Wang, Haihua
Zhao, Yao
Kong, Lihua
Wang, Haiying
Wang, Lin
Hu, Zhenbo
Xu, Xin
author_sort Wang, Jialing
collection PubMed
description The histone demethylase JMJD1C is associated with human platelet counts. The JMJD1C knockout in zebrafish and mice leads to the ablation of megakaryocyte–erythroid lineage anemia. However, the specific expression, function, and mechanism of JMJD1C in megakaryopoiesis remain unknown. Here, we used cell line models, cord blood cells, and thrombocytopenia samples, to detect the JMJD1C expression. ShRNA of JMJD1C and a specific peptide agonist of JMJD1C, SAH-JZ3, were used to explore the JMJD1C function in the cell line models. The actin ratio in megakaryopoiesis for the JMJDC modulation was also measured. Mass spectrometry was used to identify the JMJD1C-interacting proteins. We first show the JMJD1C expression difference in the PMA-induced cell line models, the thrombopoietin (TPO)-induced megakaryocyte differentiation of the cord blood cells, and also the thrombocytopenia patients, compared to the normal controls. The ShRNA of JMJD1C and SAH-JZ3 showed different effects, which were consistent with the expression of JMJD1C in the cell line models. The effort to find the underlying mechanism of JMJD1C in megakaryopoiesis, led to the discovery that SAH-JZ3 decreases F-actin in K562 cells and increases F-actin in MEG-01 cells. We further performed mass spectrometry to identify the potential JMJD1C-interacting proteins and found that the important Ran GTPase interacts with JMJD1C. To sum up, JMJD1C probably regulates megakaryopoiesis by influencing the actin network.
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spelling pubmed-96884142022-11-25 JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network Wang, Jialing Liu, Xiaodan Wang, Haixia Qin, Lili Feng, Anhua Qi, Daoxin Wang, Haihua Zhao, Yao Kong, Lihua Wang, Haiying Wang, Lin Hu, Zhenbo Xu, Xin Cells Article The histone demethylase JMJD1C is associated with human platelet counts. The JMJD1C knockout in zebrafish and mice leads to the ablation of megakaryocyte–erythroid lineage anemia. However, the specific expression, function, and mechanism of JMJD1C in megakaryopoiesis remain unknown. Here, we used cell line models, cord blood cells, and thrombocytopenia samples, to detect the JMJD1C expression. ShRNA of JMJD1C and a specific peptide agonist of JMJD1C, SAH-JZ3, were used to explore the JMJD1C function in the cell line models. The actin ratio in megakaryopoiesis for the JMJDC modulation was also measured. Mass spectrometry was used to identify the JMJD1C-interacting proteins. We first show the JMJD1C expression difference in the PMA-induced cell line models, the thrombopoietin (TPO)-induced megakaryocyte differentiation of the cord blood cells, and also the thrombocytopenia patients, compared to the normal controls. The ShRNA of JMJD1C and SAH-JZ3 showed different effects, which were consistent with the expression of JMJD1C in the cell line models. The effort to find the underlying mechanism of JMJD1C in megakaryopoiesis, led to the discovery that SAH-JZ3 decreases F-actin in K562 cells and increases F-actin in MEG-01 cells. We further performed mass spectrometry to identify the potential JMJD1C-interacting proteins and found that the important Ran GTPase interacts with JMJD1C. To sum up, JMJD1C probably regulates megakaryopoiesis by influencing the actin network. MDPI 2022-11-18 /pmc/articles/PMC9688414/ /pubmed/36429088 http://dx.doi.org/10.3390/cells11223660 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jialing
Liu, Xiaodan
Wang, Haixia
Qin, Lili
Feng, Anhua
Qi, Daoxin
Wang, Haihua
Zhao, Yao
Kong, Lihua
Wang, Haiying
Wang, Lin
Hu, Zhenbo
Xu, Xin
JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
title JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
title_full JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
title_fullStr JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
title_full_unstemmed JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
title_short JMJD1C Regulates Megakaryopoiesis in In Vitro Models through the Actin Network
title_sort jmjd1c regulates megakaryopoiesis in in vitro models through the actin network
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688414/
https://www.ncbi.nlm.nih.gov/pubmed/36429088
http://dx.doi.org/10.3390/cells11223660
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