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Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery

Adipose tissue (AT) expansion either through hypertrophy or hyperplasia is determinant in the link between obesity and metabolic alteration. The present study aims to profile the unhealthy subcutaneous and visceral AT (SAT, VAT) expansion in obesity and in the outcomes of bariatric surgery (BS). The...

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Autores principales: Ledoux, Séverine, Boulet, Nathalie, Belles, Chloé, Zakaroff-Girard, Alexia, Bernard, Arnaud, Germain, Albéric, Decaunes, Pauline, Briot, Anaïs, Galitzky, Jean, Bouloumié, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688588/
https://www.ncbi.nlm.nih.gov/pubmed/36428969
http://dx.doi.org/10.3390/cells11223540
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author Ledoux, Séverine
Boulet, Nathalie
Belles, Chloé
Zakaroff-Girard, Alexia
Bernard, Arnaud
Germain, Albéric
Decaunes, Pauline
Briot, Anaïs
Galitzky, Jean
Bouloumié, Anne
author_facet Ledoux, Séverine
Boulet, Nathalie
Belles, Chloé
Zakaroff-Girard, Alexia
Bernard, Arnaud
Germain, Albéric
Decaunes, Pauline
Briot, Anaïs
Galitzky, Jean
Bouloumié, Anne
author_sort Ledoux, Séverine
collection PubMed
description Adipose tissue (AT) expansion either through hypertrophy or hyperplasia is determinant in the link between obesity and metabolic alteration. The present study aims to profile the unhealthy subcutaneous and visceral AT (SAT, VAT) expansion in obesity and in the outcomes of bariatric surgery (BS). The repartition of adipocytes according to diameter and the numbers of progenitor subtypes and immune cells of SAT and VAT from 161 obese patients were determined by cell imaging and flow cytometry, respectively. Associations with insulin resistance (IR) prior to BS as well as with the loss of excessive weight (EWL) and IR at 1 and 3 years post-BS were studied; prior to BS, SAT and VAT, unhealthy expansions are characterized by the accumulation of adipogenic progenitors and CD4+ T lymphocytes and by adipocyte hypertrophy and elevated macrophage numbers, respectively. Such SAT stromal profile and VAT adipocyte hypertrophy are associated with adverse BS outcomes. Finally, myofibrogenic progenitors are a common determinant of weight and IR trajectories post-BS; the study suggests that adipogenesis in SAT and adipocyte hypertrophy in VAT are common determinants of metabolic alterations with obesity and of the weight loss and metabolic response to bariatric surgery. The data open up new avenues to better understand and predict individual outcomes in response to changes in energy balance.
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spelling pubmed-96885882022-11-25 Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery Ledoux, Séverine Boulet, Nathalie Belles, Chloé Zakaroff-Girard, Alexia Bernard, Arnaud Germain, Albéric Decaunes, Pauline Briot, Anaïs Galitzky, Jean Bouloumié, Anne Cells Article Adipose tissue (AT) expansion either through hypertrophy or hyperplasia is determinant in the link between obesity and metabolic alteration. The present study aims to profile the unhealthy subcutaneous and visceral AT (SAT, VAT) expansion in obesity and in the outcomes of bariatric surgery (BS). The repartition of adipocytes according to diameter and the numbers of progenitor subtypes and immune cells of SAT and VAT from 161 obese patients were determined by cell imaging and flow cytometry, respectively. Associations with insulin resistance (IR) prior to BS as well as with the loss of excessive weight (EWL) and IR at 1 and 3 years post-BS were studied; prior to BS, SAT and VAT, unhealthy expansions are characterized by the accumulation of adipogenic progenitors and CD4+ T lymphocytes and by adipocyte hypertrophy and elevated macrophage numbers, respectively. Such SAT stromal profile and VAT adipocyte hypertrophy are associated with adverse BS outcomes. Finally, myofibrogenic progenitors are a common determinant of weight and IR trajectories post-BS; the study suggests that adipogenesis in SAT and adipocyte hypertrophy in VAT are common determinants of metabolic alterations with obesity and of the weight loss and metabolic response to bariatric surgery. The data open up new avenues to better understand and predict individual outcomes in response to changes in energy balance. MDPI 2022-11-09 /pmc/articles/PMC9688588/ /pubmed/36428969 http://dx.doi.org/10.3390/cells11223540 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ledoux, Séverine
Boulet, Nathalie
Belles, Chloé
Zakaroff-Girard, Alexia
Bernard, Arnaud
Germain, Albéric
Decaunes, Pauline
Briot, Anaïs
Galitzky, Jean
Bouloumié, Anne
Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery
title Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery
title_full Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery
title_fullStr Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery
title_full_unstemmed Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery
title_short Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery
title_sort subcutaneous stromal cells and visceral adipocyte size are determinants of metabolic flexibility in obesity and in response to weight loss surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688588/
https://www.ncbi.nlm.nih.gov/pubmed/36428969
http://dx.doi.org/10.3390/cells11223540
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