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Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation

In this study, we examined the proliferation capability and osteogenic and chondrogenic differentiation potential of non-hypertrophic nonunion cells (NHNCs), and the effect of Escherichia coli-derived BMP-2 (E-BMP-2) on them. We enrolled five patients with non-hypertrophic nonunion. NHNCs isolated f...

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Autores principales: Yoshikawa, Ryo, Fukui, Tomoaki, Oe, Keisuke, Kumabe, Yohei, Oda, Takahiro, Sawauchi, Kenichi, Takase, Kyohei, Yamamoto, Yuya, Sakai, Yoshitada, Kuroda, Ryosuke, Niikura, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688795/
https://www.ncbi.nlm.nih.gov/pubmed/36354689
http://dx.doi.org/10.3390/cimb44110377
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author Yoshikawa, Ryo
Fukui, Tomoaki
Oe, Keisuke
Kumabe, Yohei
Oda, Takahiro
Sawauchi, Kenichi
Takase, Kyohei
Yamamoto, Yuya
Sakai, Yoshitada
Kuroda, Ryosuke
Niikura, Takahiro
author_facet Yoshikawa, Ryo
Fukui, Tomoaki
Oe, Keisuke
Kumabe, Yohei
Oda, Takahiro
Sawauchi, Kenichi
Takase, Kyohei
Yamamoto, Yuya
Sakai, Yoshitada
Kuroda, Ryosuke
Niikura, Takahiro
author_sort Yoshikawa, Ryo
collection PubMed
description In this study, we examined the proliferation capability and osteogenic and chondrogenic differentiation potential of non-hypertrophic nonunion cells (NHNCs), and the effect of Escherichia coli-derived BMP-2 (E-BMP-2) on them. We enrolled five patients with non-hypertrophic nonunion. NHNCs isolated from nonunion tissue sampled during surgery were cultured, passaged, counted every 14 days, and analyzed. NHNCs were homogenous fibroblastic adherent cells and long-lived through at least 10 passages, with a slight decline. The cells were consistently positive for mesenchymal stem cell-related markers CD73 and CD105, and negative for the hematopoietic markers CD14 and CD45. NHNCs could differentiate into osteoblast lineage cells; however, they did not have strong calcification or sufficient chondrogenic differentiation capability. E-BMP-2 did not affect the proliferative capability of the cells but improved their osteogenic differentiation capability by increasing alkaline phosphatase activity and upregulating the gene expression of osterix, bone sialoprotein, and osteocalcin. E-BMP-2 enhanced their chondrogenic differentiation capability by upregulating the gene expression of aggrecan and collagen type II. We showed, for the first time, that NHNCs have the capacity to differentiate into osteoblast-lineage cells, although the chondrogenic differentiation potential was poor. Local application of E-BMP-2 with preservation of nonunion tissue is a potential treatment option for non-hypertrophic nonunion.
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spelling pubmed-96887952022-11-25 Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation Yoshikawa, Ryo Fukui, Tomoaki Oe, Keisuke Kumabe, Yohei Oda, Takahiro Sawauchi, Kenichi Takase, Kyohei Yamamoto, Yuya Sakai, Yoshitada Kuroda, Ryosuke Niikura, Takahiro Curr Issues Mol Biol Article In this study, we examined the proliferation capability and osteogenic and chondrogenic differentiation potential of non-hypertrophic nonunion cells (NHNCs), and the effect of Escherichia coli-derived BMP-2 (E-BMP-2) on them. We enrolled five patients with non-hypertrophic nonunion. NHNCs isolated from nonunion tissue sampled during surgery were cultured, passaged, counted every 14 days, and analyzed. NHNCs were homogenous fibroblastic adherent cells and long-lived through at least 10 passages, with a slight decline. The cells were consistently positive for mesenchymal stem cell-related markers CD73 and CD105, and negative for the hematopoietic markers CD14 and CD45. NHNCs could differentiate into osteoblast lineage cells; however, they did not have strong calcification or sufficient chondrogenic differentiation capability. E-BMP-2 did not affect the proliferative capability of the cells but improved their osteogenic differentiation capability by increasing alkaline phosphatase activity and upregulating the gene expression of osterix, bone sialoprotein, and osteocalcin. E-BMP-2 enhanced their chondrogenic differentiation capability by upregulating the gene expression of aggrecan and collagen type II. We showed, for the first time, that NHNCs have the capacity to differentiate into osteoblast-lineage cells, although the chondrogenic differentiation potential was poor. Local application of E-BMP-2 with preservation of nonunion tissue is a potential treatment option for non-hypertrophic nonunion. MDPI 2022-11-09 /pmc/articles/PMC9688795/ /pubmed/36354689 http://dx.doi.org/10.3390/cimb44110377 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoshikawa, Ryo
Fukui, Tomoaki
Oe, Keisuke
Kumabe, Yohei
Oda, Takahiro
Sawauchi, Kenichi
Takase, Kyohei
Yamamoto, Yuya
Sakai, Yoshitada
Kuroda, Ryosuke
Niikura, Takahiro
Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation
title Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation
title_full Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation
title_fullStr Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation
title_full_unstemmed Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation
title_short Human Non-Hypertrophic Nonunion Tissue Contains Osteoblast Lineage Cells and E-BMP-2 Activates Osteogenic and Chondrogenic Differentiation
title_sort human non-hypertrophic nonunion tissue contains osteoblast lineage cells and e-bmp-2 activates osteogenic and chondrogenic differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688795/
https://www.ncbi.nlm.nih.gov/pubmed/36354689
http://dx.doi.org/10.3390/cimb44110377
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