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The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei

In recent years, the information crosstalk between the central nervous system and the periphery has been a hot topic, such as the brain–gut axis, brain–lung axis, etc. Among them, some studies have shown that brainstem nuclei activity can significantly affect the progression of peripheral tumor; how...

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Autores principales: Chen, Mo, Jiao, Yingfu, Shi, Yumiao, Xu, Saihong, Tang, Dan, Chen, Sihan, Gao, Po, Zhang, Xindi, Zhao, Xiaojing, Cai, Mengmeng, Yu, Weifeng, Xie, Kangjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688822/
https://www.ncbi.nlm.nih.gov/pubmed/36358412
http://dx.doi.org/10.3390/brainsci12111486
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author Chen, Mo
Jiao, Yingfu
Shi, Yumiao
Xu, Saihong
Tang, Dan
Chen, Sihan
Gao, Po
Zhang, Xindi
Zhao, Xiaojing
Cai, Mengmeng
Yu, Weifeng
Xie, Kangjie
author_facet Chen, Mo
Jiao, Yingfu
Shi, Yumiao
Xu, Saihong
Tang, Dan
Chen, Sihan
Gao, Po
Zhang, Xindi
Zhao, Xiaojing
Cai, Mengmeng
Yu, Weifeng
Xie, Kangjie
author_sort Chen, Mo
collection PubMed
description In recent years, the information crosstalk between the central nervous system and the periphery has been a hot topic, such as the brain–gut axis, brain–lung axis, etc. Among them, some studies have shown that brainstem nuclei activity can significantly affect the progression of peripheral tumor; however, regarding lung cancer, our understanding of the basic characteristics of the lung-innervating brain nuclei responsive to lung cancer progression remains deficient. Therefore, we used the pseudorabies virus for retrograde labeling of nerves to study the neural circuits between the lung and brain. We then established a mouse orthotopic lung cancer model and used the expression of the c-Fos gene in brain regions to characterize activated brain circuits and compared these results with those of the control group. We focused on c-Fos activity in nuclei associated with retrograde tracing regions of the brainstem. We found over 16 nuclei in the whole brain with direct or indirect lung innervation through neural retrograde labeling with the pseudorabies virus. We further revealed that the neuronal activity of the rostral ventrolateral reticular nucleus (RVL), caudal nucleus of Raphe (raphe obscurus nucleus, ROb), Raphe pallidus nucleus (RPa), and ventral gigantocellular reticular nucleus (GiV) in the rostral ventromedial and lateral medulla were significantly changed in an orthotopic lung cancer mouse model by the immunostaining of c-Fos early responsive protein. Thus, the distinctive rostroventral medulla area, functionally closely related to the vagus nerve, likely plays a role in central neural interaction with peripheral lung tumors and deserves future investigation.
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spelling pubmed-96888222022-11-25 The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei Chen, Mo Jiao, Yingfu Shi, Yumiao Xu, Saihong Tang, Dan Chen, Sihan Gao, Po Zhang, Xindi Zhao, Xiaojing Cai, Mengmeng Yu, Weifeng Xie, Kangjie Brain Sci Article In recent years, the information crosstalk between the central nervous system and the periphery has been a hot topic, such as the brain–gut axis, brain–lung axis, etc. Among them, some studies have shown that brainstem nuclei activity can significantly affect the progression of peripheral tumor; however, regarding lung cancer, our understanding of the basic characteristics of the lung-innervating brain nuclei responsive to lung cancer progression remains deficient. Therefore, we used the pseudorabies virus for retrograde labeling of nerves to study the neural circuits between the lung and brain. We then established a mouse orthotopic lung cancer model and used the expression of the c-Fos gene in brain regions to characterize activated brain circuits and compared these results with those of the control group. We focused on c-Fos activity in nuclei associated with retrograde tracing regions of the brainstem. We found over 16 nuclei in the whole brain with direct or indirect lung innervation through neural retrograde labeling with the pseudorabies virus. We further revealed that the neuronal activity of the rostral ventrolateral reticular nucleus (RVL), caudal nucleus of Raphe (raphe obscurus nucleus, ROb), Raphe pallidus nucleus (RPa), and ventral gigantocellular reticular nucleus (GiV) in the rostral ventromedial and lateral medulla were significantly changed in an orthotopic lung cancer mouse model by the immunostaining of c-Fos early responsive protein. Thus, the distinctive rostroventral medulla area, functionally closely related to the vagus nerve, likely plays a role in central neural interaction with peripheral lung tumors and deserves future investigation. MDPI 2022-11-02 /pmc/articles/PMC9688822/ /pubmed/36358412 http://dx.doi.org/10.3390/brainsci12111486 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Mo
Jiao, Yingfu
Shi, Yumiao
Xu, Saihong
Tang, Dan
Chen, Sihan
Gao, Po
Zhang, Xindi
Zhao, Xiaojing
Cai, Mengmeng
Yu, Weifeng
Xie, Kangjie
The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
title The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
title_full The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
title_fullStr The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
title_full_unstemmed The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
title_short The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
title_sort rostral ventromedial and lateral medulla are the major areas responsive to lung cancer progression among brainstem lung-innervating nuclei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688822/
https://www.ncbi.nlm.nih.gov/pubmed/36358412
http://dx.doi.org/10.3390/brainsci12111486
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