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Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer

(1) Background: As the most common malignant tumor type worldwide, it is necessary to identify novel potential prognostic biomarkers to improve the poor prognosis of lung cancer. The Timeless gene, a circadian rhythm-related gene, is associated with several types of cancer. However, studies analyzin...

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Autores principales: Ye, Jishi, Chen, Jingli, Wang, Juan, Xia, Zhongyuan, Jia, Yifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688960/
https://www.ncbi.nlm.nih.gov/pubmed/36359523
http://dx.doi.org/10.3390/diagnostics12112681
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author Ye, Jishi
Chen, Jingli
Wang, Juan
Xia, Zhongyuan
Jia, Yifan
author_facet Ye, Jishi
Chen, Jingli
Wang, Juan
Xia, Zhongyuan
Jia, Yifan
author_sort Ye, Jishi
collection PubMed
description (1) Background: As the most common malignant tumor type worldwide, it is necessary to identify novel potential prognostic biomarkers to improve the poor prognosis of lung cancer. The Timeless gene, a circadian rhythm-related gene, is associated with several types of cancer. However, studies analyzing the clinical significance of the Timeless gene in patients with lung cancer are currently limited. (2) Methods: In the present study, the expression levels and prognostic potential of the Timeless gene and its co-expressed genes in different subtypes of lung cancer were explored using multiple bioinformatics approaches. The correlations between the Timeless gene and its co-expressed genes were validated using A549 and NCI-H226 cells by transfecting them with expression vectors and analyses using Western blot and reverse transcription-quantitative PCR. (3) Results: The Oncomine and GEPIA database analyses indicated that the expression of the Timeless gene was significantly higher in lung cancer as compared to that in the normal tissue. Using the UALCAN database, significant differences in Timeless gene expression were determined among different stages of lung cancer and between genders. A Kaplan–Meier plotter analysis indicated that high expression of the Timeless gene was associated with poor overall survival (OS) and progression-free survival (PFS) of patients with lung cancer. In the cBioPortal and GEPIA database analyses, extra spindle pole bodies like 1 (ESPL1) was the top correlated gene of Timeless in patients with lung cancer. Similar to the Timeless gene, high expression of the ESPL1 gene was also associated with poor OS and PFS. Of note, overexpression of the Timeless gene increased the expression level of ESPL1 at both the mRNA and protein levels. (4) Conclusion: The present study explored the clinical significance of the Timeless gene and its correlated gene ESPL1 in patients with lung cancer, thereby providing a potential therapeutic target for lung cancer.
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spelling pubmed-96889602022-11-25 Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer Ye, Jishi Chen, Jingli Wang, Juan Xia, Zhongyuan Jia, Yifan Diagnostics (Basel) Article (1) Background: As the most common malignant tumor type worldwide, it is necessary to identify novel potential prognostic biomarkers to improve the poor prognosis of lung cancer. The Timeless gene, a circadian rhythm-related gene, is associated with several types of cancer. However, studies analyzing the clinical significance of the Timeless gene in patients with lung cancer are currently limited. (2) Methods: In the present study, the expression levels and prognostic potential of the Timeless gene and its co-expressed genes in different subtypes of lung cancer were explored using multiple bioinformatics approaches. The correlations between the Timeless gene and its co-expressed genes were validated using A549 and NCI-H226 cells by transfecting them with expression vectors and analyses using Western blot and reverse transcription-quantitative PCR. (3) Results: The Oncomine and GEPIA database analyses indicated that the expression of the Timeless gene was significantly higher in lung cancer as compared to that in the normal tissue. Using the UALCAN database, significant differences in Timeless gene expression were determined among different stages of lung cancer and between genders. A Kaplan–Meier plotter analysis indicated that high expression of the Timeless gene was associated with poor overall survival (OS) and progression-free survival (PFS) of patients with lung cancer. In the cBioPortal and GEPIA database analyses, extra spindle pole bodies like 1 (ESPL1) was the top correlated gene of Timeless in patients with lung cancer. Similar to the Timeless gene, high expression of the ESPL1 gene was also associated with poor OS and PFS. Of note, overexpression of the Timeless gene increased the expression level of ESPL1 at both the mRNA and protein levels. (4) Conclusion: The present study explored the clinical significance of the Timeless gene and its correlated gene ESPL1 in patients with lung cancer, thereby providing a potential therapeutic target for lung cancer. MDPI 2022-11-03 /pmc/articles/PMC9688960/ /pubmed/36359523 http://dx.doi.org/10.3390/diagnostics12112681 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ye, Jishi
Chen, Jingli
Wang, Juan
Xia, Zhongyuan
Jia, Yifan
Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer
title Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer
title_full Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer
title_fullStr Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer
title_full_unstemmed Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer
title_short Association of the Timeless Gene with Prognosis and Clinical Characteristics of Human Lung Cancer
title_sort association of the timeless gene with prognosis and clinical characteristics of human lung cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688960/
https://www.ncbi.nlm.nih.gov/pubmed/36359523
http://dx.doi.org/10.3390/diagnostics12112681
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