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High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma

Objective: Kruppel-associated box (KRAB) proteins reportedly play a dual role in neoplastic transformation. At present, little is known about the function of the proteins encoded by the human pogo transposable element derived with KRAB domain (POGK) gene. Herein, we evaluated the prognostic signific...

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Autores principales: Xu, Wenxiong, Huang, Yanlin, Mei, Yongyu, Zhang, Yeqiong, Luo, Qiumin, Zhu, Shu, Peng, Liang, Gao, Zhiliang, Liu, Ying, Li, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688978/
https://www.ncbi.nlm.nih.gov/pubmed/36421335
http://dx.doi.org/10.3390/curroncol29110682
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author Xu, Wenxiong
Huang, Yanlin
Mei, Yongyu
Zhang, Yeqiong
Luo, Qiumin
Zhu, Shu
Peng, Liang
Gao, Zhiliang
Liu, Ying
Li, Jianguo
author_facet Xu, Wenxiong
Huang, Yanlin
Mei, Yongyu
Zhang, Yeqiong
Luo, Qiumin
Zhu, Shu
Peng, Liang
Gao, Zhiliang
Liu, Ying
Li, Jianguo
author_sort Xu, Wenxiong
collection PubMed
description Objective: Kruppel-associated box (KRAB) proteins reportedly play a dual role in neoplastic transformation. At present, little is known about the function of the proteins encoded by the human pogo transposable element derived with KRAB domain (POGK) gene. Herein, we evaluated the prognostic significance of POGK expression in patients with hepatocellular carcinoma (HCC). Methods: The data of HCC patients was downloaded from The Cancer Genome Atlas (TCGA) database. To determine the relationship between POGK and clinical features, logistic regression was applied. Cox regression and Kaplan-Meier analyses were used to evaluate the correlation between POGK and survival rates. Gene ontology (GO) analysis and Gene set enrichment analysis (GSEA) were conducted to identify the enriched pathways and functions associated with POGK. Results: A total of 374 HCC patients were identified in TCGA. POGK was significantly upregulated in HCC and correlated with tumor status (p = 0.036), race (p = 0.025), weight (p = 0.002), body mass index (p = 0.033), histologic grade (p < 0.001), and alpha-fetoprotein (p < 0.001). High POGK expression in HCC patients correlated with a poor outcome in terms of overall survival (p = 0.0018), progression-free survival (p = 0.0087), relapse-free survival (p = 0.045), and disease-specific survival (p = 0.014), according to Kaplan-Meier analysis. Receiver operating characteristic curve analysis showed that the area under the curve of POGK expression for HCC diagnosis was 0.891. GSEA showed that high POGK expression might activate mitotic prometaphase, kinesins, homologous DNA pairing and strand exchange, MET activates PTK2 signaling pathway, G1 to S cell cycle control, Aurora B pathway, ncRNAs involved in WNT signaling pathway, hepatitis C, and ncRNAs involved in the STAT3 signaling pathway. POGK expression correlated with the abundance of adaptive and innate immunocytes in HCC. Conclusion: High expression of POGK has high diagnostic and prognostic values in patients with HCC. Moreover, POGK expression is correlated with immune infiltration in HCC.
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spelling pubmed-96889782022-11-25 High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma Xu, Wenxiong Huang, Yanlin Mei, Yongyu Zhang, Yeqiong Luo, Qiumin Zhu, Shu Peng, Liang Gao, Zhiliang Liu, Ying Li, Jianguo Curr Oncol Article Objective: Kruppel-associated box (KRAB) proteins reportedly play a dual role in neoplastic transformation. At present, little is known about the function of the proteins encoded by the human pogo transposable element derived with KRAB domain (POGK) gene. Herein, we evaluated the prognostic significance of POGK expression in patients with hepatocellular carcinoma (HCC). Methods: The data of HCC patients was downloaded from The Cancer Genome Atlas (TCGA) database. To determine the relationship between POGK and clinical features, logistic regression was applied. Cox regression and Kaplan-Meier analyses were used to evaluate the correlation between POGK and survival rates. Gene ontology (GO) analysis and Gene set enrichment analysis (GSEA) were conducted to identify the enriched pathways and functions associated with POGK. Results: A total of 374 HCC patients were identified in TCGA. POGK was significantly upregulated in HCC and correlated with tumor status (p = 0.036), race (p = 0.025), weight (p = 0.002), body mass index (p = 0.033), histologic grade (p < 0.001), and alpha-fetoprotein (p < 0.001). High POGK expression in HCC patients correlated with a poor outcome in terms of overall survival (p = 0.0018), progression-free survival (p = 0.0087), relapse-free survival (p = 0.045), and disease-specific survival (p = 0.014), according to Kaplan-Meier analysis. Receiver operating characteristic curve analysis showed that the area under the curve of POGK expression for HCC diagnosis was 0.891. GSEA showed that high POGK expression might activate mitotic prometaphase, kinesins, homologous DNA pairing and strand exchange, MET activates PTK2 signaling pathway, G1 to S cell cycle control, Aurora B pathway, ncRNAs involved in WNT signaling pathway, hepatitis C, and ncRNAs involved in the STAT3 signaling pathway. POGK expression correlated with the abundance of adaptive and innate immunocytes in HCC. Conclusion: High expression of POGK has high diagnostic and prognostic values in patients with HCC. Moreover, POGK expression is correlated with immune infiltration in HCC. MDPI 2022-11-14 /pmc/articles/PMC9688978/ /pubmed/36421335 http://dx.doi.org/10.3390/curroncol29110682 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Wenxiong
Huang, Yanlin
Mei, Yongyu
Zhang, Yeqiong
Luo, Qiumin
Zhu, Shu
Peng, Liang
Gao, Zhiliang
Liu, Ying
Li, Jianguo
High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma
title High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma
title_full High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma
title_fullStr High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma
title_full_unstemmed High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma
title_short High Expression of POGK Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma
title_sort high expression of pogk predicts poor prognosis in patients with hepatocellular carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688978/
https://www.ncbi.nlm.nih.gov/pubmed/36421335
http://dx.doi.org/10.3390/curroncol29110682
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