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Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel

Due to the expansion of point-of-care devices, proposing a convenient and efficient method for blood–plasma separation would help with the use of point-of-care devices. Commercial microfluidic chips are only able to separate a limited amount of plasma, and the majority of these chips need an active...

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Autores principales: Hatami, Ali, Saadatmand, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689033/
https://www.ncbi.nlm.nih.gov/pubmed/36428933
http://dx.doi.org/10.3390/diagnostics12112873
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author Hatami, Ali
Saadatmand, Maryam
author_facet Hatami, Ali
Saadatmand, Maryam
author_sort Hatami, Ali
collection PubMed
description Due to the expansion of point-of-care devices, proposing a convenient and efficient method for blood–plasma separation would help with the use of point-of-care devices. Commercial microfluidic chips are only able to separate a limited amount of plasma, and the majority of these chips need an active valve system, which leads to increase manufacturing cost and complexity. In this research study, we designed a centrifugal microfluidic disk with a passive valve for ultra-accurate and efficient blood–plasma separation on a large scale (2–3 mL). The disk contained a separator gel, which, after applying the centrifugal force, separated the plasma and red blood cells. The passive valve worked based on the inertial force and was able to transfer more than 90% of the separated plasma to the next chamber. The results demonstrated that the separated plasma was 99.992% pure. This study compared the efficiency of the disk containing separating gel with the common lab-on-a-disk design for plasma separation. A comparison of the results showed that although the common lab-on-a-disk design could separate almost pure plasma as the disk contained separator gel, it could only transfer 60% of plasma to the next chamber.
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spelling pubmed-96890332022-11-25 Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel Hatami, Ali Saadatmand, Maryam Diagnostics (Basel) Article Due to the expansion of point-of-care devices, proposing a convenient and efficient method for blood–plasma separation would help with the use of point-of-care devices. Commercial microfluidic chips are only able to separate a limited amount of plasma, and the majority of these chips need an active valve system, which leads to increase manufacturing cost and complexity. In this research study, we designed a centrifugal microfluidic disk with a passive valve for ultra-accurate and efficient blood–plasma separation on a large scale (2–3 mL). The disk contained a separator gel, which, after applying the centrifugal force, separated the plasma and red blood cells. The passive valve worked based on the inertial force and was able to transfer more than 90% of the separated plasma to the next chamber. The results demonstrated that the separated plasma was 99.992% pure. This study compared the efficiency of the disk containing separating gel with the common lab-on-a-disk design for plasma separation. A comparison of the results showed that although the common lab-on-a-disk design could separate almost pure plasma as the disk contained separator gel, it could only transfer 60% of plasma to the next chamber. MDPI 2022-11-20 /pmc/articles/PMC9689033/ /pubmed/36428933 http://dx.doi.org/10.3390/diagnostics12112873 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hatami, Ali
Saadatmand, Maryam
Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel
title Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel
title_full Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel
title_fullStr Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel
title_full_unstemmed Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel
title_short Extremely Precise Blood–Plasma Separation from Whole Blood on a Centrifugal Microfluidic Disk (Lab-on-a-Disk) Using Separator Gel
title_sort extremely precise blood–plasma separation from whole blood on a centrifugal microfluidic disk (lab-on-a-disk) using separator gel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689033/
https://www.ncbi.nlm.nih.gov/pubmed/36428933
http://dx.doi.org/10.3390/diagnostics12112873
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