Cargando…

Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis

Adenomyosis is a uterine pathology characterized by a deep invasion of endometrial glands and stroma, disrupting the endometrial–myometrial interface (EMI). Interleukin-18 (IL-18) system is a dominant cytokine involved in the menstrual cycle of human endometrium. IL-18 may play a defensive role agai...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Liang-Hsuan, Chan, She-Hung, Li, Chin-Jung, Wu, Hsien-Ming, Huang, Hong-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689074/
https://www.ncbi.nlm.nih.gov/pubmed/36354688
http://dx.doi.org/10.3390/cimb44110376
_version_ 1784836433325850624
author Chen, Liang-Hsuan
Chan, She-Hung
Li, Chin-Jung
Wu, Hsien-Ming
Huang, Hong-Yuan
author_facet Chen, Liang-Hsuan
Chan, She-Hung
Li, Chin-Jung
Wu, Hsien-Ming
Huang, Hong-Yuan
author_sort Chen, Liang-Hsuan
collection PubMed
description Adenomyosis is a uterine pathology characterized by a deep invasion of endometrial glands and stroma, disrupting the endometrial–myometrial interface (EMI). Interleukin-18 (IL-18) system is a dominant cytokine involved in the menstrual cycle of human endometrium. IL-18 may play a defensive role against maternal immune response in the uterine cavity. The present study was designed to determine IL-18-mediated immune response at the level of EMI. We uncovered that mRNA of IL-18 system, including IL-18, IL-18 receptor (IL-18R), and its antagonist, IL-18 binding protein (IL-18BP), expressed in eutopic, ectopic endometrium, and corresponding myometrium in patients with adenomyosis. IL-18 system was demonstrated in paired tissue samples by immunochemistry and immunofluorescence study. According to RT-PCR with C(T) value quantification and 2(−∆∆Ct) method, a significant down-regulation of IL-18BP in corresponding myometrium in comparison to eutopic endometrium (p < 0.05) indicates that the IL-18 system acts as a local immune modulator at the level of EMI and regulating cytokine networks in the pathogenesis of adenomyosis. Furthermore, an increased IL-18 antagonist to agonist ratio was noted in ectopic endometrium compared with corresponding myometrium. We suggest that altered IL-18 system expression contributes to immunological dysfunction and junctional zone disturbance in women with adenomyosis.
format Online
Article
Text
id pubmed-9689074
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96890742022-11-25 Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis Chen, Liang-Hsuan Chan, She-Hung Li, Chin-Jung Wu, Hsien-Ming Huang, Hong-Yuan Curr Issues Mol Biol Article Adenomyosis is a uterine pathology characterized by a deep invasion of endometrial glands and stroma, disrupting the endometrial–myometrial interface (EMI). Interleukin-18 (IL-18) system is a dominant cytokine involved in the menstrual cycle of human endometrium. IL-18 may play a defensive role against maternal immune response in the uterine cavity. The present study was designed to determine IL-18-mediated immune response at the level of EMI. We uncovered that mRNA of IL-18 system, including IL-18, IL-18 receptor (IL-18R), and its antagonist, IL-18 binding protein (IL-18BP), expressed in eutopic, ectopic endometrium, and corresponding myometrium in patients with adenomyosis. IL-18 system was demonstrated in paired tissue samples by immunochemistry and immunofluorescence study. According to RT-PCR with C(T) value quantification and 2(−∆∆Ct) method, a significant down-regulation of IL-18BP in corresponding myometrium in comparison to eutopic endometrium (p < 0.05) indicates that the IL-18 system acts as a local immune modulator at the level of EMI and regulating cytokine networks in the pathogenesis of adenomyosis. Furthermore, an increased IL-18 antagonist to agonist ratio was noted in ectopic endometrium compared with corresponding myometrium. We suggest that altered IL-18 system expression contributes to immunological dysfunction and junctional zone disturbance in women with adenomyosis. MDPI 2022-11-09 /pmc/articles/PMC9689074/ /pubmed/36354688 http://dx.doi.org/10.3390/cimb44110376 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Liang-Hsuan
Chan, She-Hung
Li, Chin-Jung
Wu, Hsien-Ming
Huang, Hong-Yuan
Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis
title Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis
title_full Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis
title_fullStr Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis
title_full_unstemmed Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis
title_short Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial Myometrial Interface in Women with Adenomyosis
title_sort altered expression of interleukin-18 system mrna at the level of endometrial myometrial interface in women with adenomyosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689074/
https://www.ncbi.nlm.nih.gov/pubmed/36354688
http://dx.doi.org/10.3390/cimb44110376
work_keys_str_mv AT chenlianghsuan alteredexpressionofinterleukin18systemmrnaatthelevelofendometrialmyometrialinterfaceinwomenwithadenomyosis
AT chanshehung alteredexpressionofinterleukin18systemmrnaatthelevelofendometrialmyometrialinterfaceinwomenwithadenomyosis
AT lichinjung alteredexpressionofinterleukin18systemmrnaatthelevelofendometrialmyometrialinterfaceinwomenwithadenomyosis
AT wuhsienming alteredexpressionofinterleukin18systemmrnaatthelevelofendometrialmyometrialinterfaceinwomenwithadenomyosis
AT huanghongyuan alteredexpressionofinterleukin18systemmrnaatthelevelofendometrialmyometrialinterfaceinwomenwithadenomyosis