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Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus

Hepatitis B virus (HBV) infection is a serious global health problem. Patients with autoimmune diseases, such as Lupus Erythematosus, are exposed to a higher risk of acquiring infections. In this study, a molecular characterization, genomic investigation of the Hepatitis B virus, polymerase (P) and...

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Autores principales: Villano, Umbertina, Mataj, Elida, Dorrucci, Maria, Farchi, Francesca, Pirone, Carmelo, Valdarchi, Catia, Equestre, Michele, Madonna, Elisabetta, Bruni, Roberto, Pisani, Giulio, Martina, Antonio, Simeoni, Matteo, Iaiani, Giancarlo, Ciccozzi, Massimo, Ciccaglione, Anna Rita, Conti, Fabrizio, Ceccarelli, Fulvia, Lo Presti, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689093/
https://www.ncbi.nlm.nih.gov/pubmed/36428926
http://dx.doi.org/10.3390/diagnostics12112866
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author Villano, Umbertina
Mataj, Elida
Dorrucci, Maria
Farchi, Francesca
Pirone, Carmelo
Valdarchi, Catia
Equestre, Michele
Madonna, Elisabetta
Bruni, Roberto
Pisani, Giulio
Martina, Antonio
Simeoni, Matteo
Iaiani, Giancarlo
Ciccozzi, Massimo
Ciccaglione, Anna Rita
Conti, Fabrizio
Ceccarelli, Fulvia
Lo Presti, Alessandra
author_facet Villano, Umbertina
Mataj, Elida
Dorrucci, Maria
Farchi, Francesca
Pirone, Carmelo
Valdarchi, Catia
Equestre, Michele
Madonna, Elisabetta
Bruni, Roberto
Pisani, Giulio
Martina, Antonio
Simeoni, Matteo
Iaiani, Giancarlo
Ciccozzi, Massimo
Ciccaglione, Anna Rita
Conti, Fabrizio
Ceccarelli, Fulvia
Lo Presti, Alessandra
author_sort Villano, Umbertina
collection PubMed
description Hepatitis B virus (HBV) infection is a serious global health problem. Patients with autoimmune diseases, such as Lupus Erythematosus, are exposed to a higher risk of acquiring infections. In this study, a molecular characterization, genomic investigation of the Hepatitis B virus, polymerase (P) and surface (S) genes, from a patient affected by Cutaneous Lupus Erythematosus (CLE), was presented. Viral DNA was extracted from 200 μL of serum, and the HBV-DNA was amplified by real-time polymerase chain reaction (PCR) with the Platinum Taq DNA Polymerase. The PCR products were purified and sequencing reactions were performed. A phylogenetic analysis was performed through maximum likelihood and Bayesian approaches. The HBV CLE isolate was classified as sub-genotype D3 and related to other Italian HBV D3 genomes, and some from foreign countries. No drug resistant mutations were identified. One mutation (a.a. 168 M) was located in the last part of the major hydrophilic region (MHR) of the surface antigen (HBsAg). Moreover, three sites (351G, 526Y, 578C) in the polymerase were exclusively present in the CLE patient. The mutations identified exclusively in the HBsAg of our CLE patient may have been selected because of the Lupus autoantibodies, which are characteristic in the Lupus autoimmune disease, using a possible molecular mimicry mechanism.
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spelling pubmed-96890932022-11-25 Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus Villano, Umbertina Mataj, Elida Dorrucci, Maria Farchi, Francesca Pirone, Carmelo Valdarchi, Catia Equestre, Michele Madonna, Elisabetta Bruni, Roberto Pisani, Giulio Martina, Antonio Simeoni, Matteo Iaiani, Giancarlo Ciccozzi, Massimo Ciccaglione, Anna Rita Conti, Fabrizio Ceccarelli, Fulvia Lo Presti, Alessandra Diagnostics (Basel) Case Report Hepatitis B virus (HBV) infection is a serious global health problem. Patients with autoimmune diseases, such as Lupus Erythematosus, are exposed to a higher risk of acquiring infections. In this study, a molecular characterization, genomic investigation of the Hepatitis B virus, polymerase (P) and surface (S) genes, from a patient affected by Cutaneous Lupus Erythematosus (CLE), was presented. Viral DNA was extracted from 200 μL of serum, and the HBV-DNA was amplified by real-time polymerase chain reaction (PCR) with the Platinum Taq DNA Polymerase. The PCR products were purified and sequencing reactions were performed. A phylogenetic analysis was performed through maximum likelihood and Bayesian approaches. The HBV CLE isolate was classified as sub-genotype D3 and related to other Italian HBV D3 genomes, and some from foreign countries. No drug resistant mutations were identified. One mutation (a.a. 168 M) was located in the last part of the major hydrophilic region (MHR) of the surface antigen (HBsAg). Moreover, three sites (351G, 526Y, 578C) in the polymerase were exclusively present in the CLE patient. The mutations identified exclusively in the HBsAg of our CLE patient may have been selected because of the Lupus autoantibodies, which are characteristic in the Lupus autoimmune disease, using a possible molecular mimicry mechanism. MDPI 2022-11-19 /pmc/articles/PMC9689093/ /pubmed/36428926 http://dx.doi.org/10.3390/diagnostics12112866 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Villano, Umbertina
Mataj, Elida
Dorrucci, Maria
Farchi, Francesca
Pirone, Carmelo
Valdarchi, Catia
Equestre, Michele
Madonna, Elisabetta
Bruni, Roberto
Pisani, Giulio
Martina, Antonio
Simeoni, Matteo
Iaiani, Giancarlo
Ciccozzi, Massimo
Ciccaglione, Anna Rita
Conti, Fabrizio
Ceccarelli, Fulvia
Lo Presti, Alessandra
Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus
title Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus
title_full Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus
title_fullStr Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus
title_full_unstemmed Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus
title_short Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus
title_sort molecular characterization of hepatitis b virus infection in a patient with cutaneous lupus erythematosus
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689093/
https://www.ncbi.nlm.nih.gov/pubmed/36428926
http://dx.doi.org/10.3390/diagnostics12112866
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