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Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach

The species spectrum of the oral microbiome is considered to be the key factor in the development and progression of periodontal inflammatory disorders. The “red complex” including Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola has the highest pathogenic potential. These bact...

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Autores principales: Aronova, Ekaterina, Dmitrienko, Marina, Ivanova, Anastasija, Gaykova, Yulia, Kurochkina, Anna, Blinova, Alisa, Bazarnova, Julia, Paponova, Elizaveta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689137/
https://www.ncbi.nlm.nih.gov/pubmed/36421404
http://dx.doi.org/10.3390/dj10110217
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author Aronova, Ekaterina
Dmitrienko, Marina
Ivanova, Anastasija
Gaykova, Yulia
Kurochkina, Anna
Blinova, Alisa
Bazarnova, Julia
Paponova, Elizaveta
author_facet Aronova, Ekaterina
Dmitrienko, Marina
Ivanova, Anastasija
Gaykova, Yulia
Kurochkina, Anna
Blinova, Alisa
Bazarnova, Julia
Paponova, Elizaveta
author_sort Aronova, Ekaterina
collection PubMed
description The species spectrum of the oral microbiome is considered to be the key factor in the development and progression of periodontal inflammatory disorders. The “red complex” including Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola has the highest pathogenic potential. These bacteria have several biochemical mechanisms that allow them to colonize and destroy periodontal tissues. Proteolytic enzymes play a crucial role in this process. Early diagnosis of pathological conditions induced by microbial contamination allows for the timely treatment of periodontitis. Otherwise, the development of the disease may lead to tooth loss. A total of 48 patients aged 18 to 65 years old who required professional oral hygiene were recruited for this clinical study. Microbial content analysis of dental plaque from the interdental space and the back of the tongue was performed using real-time PCR. To determine the proteolytic activity of oral bacteria, the new express diagnostic method was applied (diagnostic sensitivity, 0.875; specificity, 0.928). The results demonstrate a strong and significant correlation between the new method and the PCR analysis (r = 0.785, p < 0.001). These results show that the new express method can be valuable as an early diagnostic method for periodontal inflammatory disorders caused by the “red complex” bacteria.
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spelling pubmed-96891372022-11-25 Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach Aronova, Ekaterina Dmitrienko, Marina Ivanova, Anastasija Gaykova, Yulia Kurochkina, Anna Blinova, Alisa Bazarnova, Julia Paponova, Elizaveta Dent J (Basel) Article The species spectrum of the oral microbiome is considered to be the key factor in the development and progression of periodontal inflammatory disorders. The “red complex” including Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola has the highest pathogenic potential. These bacteria have several biochemical mechanisms that allow them to colonize and destroy periodontal tissues. Proteolytic enzymes play a crucial role in this process. Early diagnosis of pathological conditions induced by microbial contamination allows for the timely treatment of periodontitis. Otherwise, the development of the disease may lead to tooth loss. A total of 48 patients aged 18 to 65 years old who required professional oral hygiene were recruited for this clinical study. Microbial content analysis of dental plaque from the interdental space and the back of the tongue was performed using real-time PCR. To determine the proteolytic activity of oral bacteria, the new express diagnostic method was applied (diagnostic sensitivity, 0.875; specificity, 0.928). The results demonstrate a strong and significant correlation between the new method and the PCR analysis (r = 0.785, p < 0.001). These results show that the new express method can be valuable as an early diagnostic method for periodontal inflammatory disorders caused by the “red complex” bacteria. MDPI 2022-11-21 /pmc/articles/PMC9689137/ /pubmed/36421404 http://dx.doi.org/10.3390/dj10110217 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aronova, Ekaterina
Dmitrienko, Marina
Ivanova, Anastasija
Gaykova, Yulia
Kurochkina, Anna
Blinova, Alisa
Bazarnova, Julia
Paponova, Elizaveta
Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach
title Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach
title_full Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach
title_fullStr Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach
title_full_unstemmed Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach
title_short Express Diagnostics of Proteolytic Activity of Periodontopathogens—Methodological Approach
title_sort express diagnostics of proteolytic activity of periodontopathogens—methodological approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689137/
https://www.ncbi.nlm.nih.gov/pubmed/36421404
http://dx.doi.org/10.3390/dj10110217
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