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Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva

The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for spe...

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Autores principales: Tokuyama-Toda, Reiko, Terada-Ito, Chika, Muraoka, Masaaki, Horiuchi, Toshikatsu, Amemiya, Tsuyoshi, Fukuoka, Airi, Hamada, Yoshiki, Takebe, Yusuke, Ogawa, Takashi, Fujii, Seiko, Kikuta, Toshihiro, Sejima, Shunsuke, Satomura, Kazuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689209/
https://www.ncbi.nlm.nih.gov/pubmed/36359412
http://dx.doi.org/10.3390/diagnostics12112568
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author Tokuyama-Toda, Reiko
Terada-Ito, Chika
Muraoka, Masaaki
Horiuchi, Toshikatsu
Amemiya, Tsuyoshi
Fukuoka, Airi
Hamada, Yoshiki
Takebe, Yusuke
Ogawa, Takashi
Fujii, Seiko
Kikuta, Toshihiro
Sejima, Shunsuke
Satomura, Kazuhito
author_facet Tokuyama-Toda, Reiko
Terada-Ito, Chika
Muraoka, Masaaki
Horiuchi, Toshikatsu
Amemiya, Tsuyoshi
Fukuoka, Airi
Hamada, Yoshiki
Takebe, Yusuke
Ogawa, Takashi
Fujii, Seiko
Kikuta, Toshihiro
Sejima, Shunsuke
Satomura, Kazuhito
author_sort Tokuyama-Toda, Reiko
collection PubMed
description The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for specialized laboratory technicians and a long turnaround time. Therefore, we have established and reported a rapid diagnostic method using a small amount of saliva as a sample using a lightweight mobile qPCR device. This study aimed to improve the existing method and increase the detection sensitivity and specificity. The detection specificity of CDC N1 and N2 was examined by improving qPCR reagents and polymerase chain reaction conditions for the previously reported method. Furthermore, the feasibility of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA was examined using both the previous method and the improved method in patients with COVID-19. The results showed that the improved method increased the specificity and sensitivity. This improved method is useful for the rapid diagnosis of SARS-CoV-2.
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spelling pubmed-96892092022-11-25 Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva Tokuyama-Toda, Reiko Terada-Ito, Chika Muraoka, Masaaki Horiuchi, Toshikatsu Amemiya, Tsuyoshi Fukuoka, Airi Hamada, Yoshiki Takebe, Yusuke Ogawa, Takashi Fujii, Seiko Kikuta, Toshihiro Sejima, Shunsuke Satomura, Kazuhito Diagnostics (Basel) Communication The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for specialized laboratory technicians and a long turnaround time. Therefore, we have established and reported a rapid diagnostic method using a small amount of saliva as a sample using a lightweight mobile qPCR device. This study aimed to improve the existing method and increase the detection sensitivity and specificity. The detection specificity of CDC N1 and N2 was examined by improving qPCR reagents and polymerase chain reaction conditions for the previously reported method. Furthermore, the feasibility of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA was examined using both the previous method and the improved method in patients with COVID-19. The results showed that the improved method increased the specificity and sensitivity. This improved method is useful for the rapid diagnosis of SARS-CoV-2. MDPI 2022-10-22 /pmc/articles/PMC9689209/ /pubmed/36359412 http://dx.doi.org/10.3390/diagnostics12112568 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Tokuyama-Toda, Reiko
Terada-Ito, Chika
Muraoka, Masaaki
Horiuchi, Toshikatsu
Amemiya, Tsuyoshi
Fukuoka, Airi
Hamada, Yoshiki
Takebe, Yusuke
Ogawa, Takashi
Fujii, Seiko
Kikuta, Toshihiro
Sejima, Shunsuke
Satomura, Kazuhito
Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
title Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
title_full Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
title_fullStr Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
title_full_unstemmed Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
title_short Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
title_sort improving the detection sensitivity of a new rapid diagnostic technology for severe acute respiratory syndrome coronavirus 2 using a trace amount of saliva
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689209/
https://www.ncbi.nlm.nih.gov/pubmed/36359412
http://dx.doi.org/10.3390/diagnostics12112568
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