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HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker?
Background: The high-temperature requirement A 1 (HtrA1) is a multidomain secretory protein with serine-protease activity, expressed in many tissues, including placenta, where its expression is higher in the first trimester, suggesting an association of this serine protease in early phases of human...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689498/ https://www.ncbi.nlm.nih.gov/pubmed/36359548 http://dx.doi.org/10.3390/diagnostics12112705 |
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author | Tossetta, Giovanni Fantone, Sonia Gesuita, Rosaria Di Renzo, Gian Carlo Meyyazhagan, Arun Tersigni, Chiara Scambia, Giovanni Di Simone, Nicoletta Marzioni, Daniela |
author_facet | Tossetta, Giovanni Fantone, Sonia Gesuita, Rosaria Di Renzo, Gian Carlo Meyyazhagan, Arun Tersigni, Chiara Scambia, Giovanni Di Simone, Nicoletta Marzioni, Daniela |
author_sort | Tossetta, Giovanni |
collection | PubMed |
description | Background: The high-temperature requirement A 1 (HtrA1) is a multidomain secretory protein with serine-protease activity, expressed in many tissues, including placenta, where its expression is higher in the first trimester, suggesting an association of this serine protease in early phases of human placenta development. In this study, we evaluated maternal serum HtrA1 levels in the first and third trimester of gestation. In particular, we evaluated a possible role of HtrA1 as an early marker of gestational diabetes mellitus (GDM) in the first trimester of gestation. Methods: We evaluated HtrA1 serum levels in the third trimester (36–40 weeks) in normal pregnancies (n = 20) and GDM pregnancies (n = 20) by using ELISA analysis. Secondly, we performed the same analysis by using the first trimester sera (10–12 weeks) of healthy pregnant women that will develop a normal pregnancy (n = 210) or GDM (n = 28) during pregnancy. Results: We found that HtrA1 serum levels in the third trimester were higher in pregnancies complicated by GDM. Interestingly, higher HtrA1 serum levels were also found in the first trimester in women developing GDM later during the second–third trimester. No significant differences in terms of maternal age and gestational age were found between cases and controls. Women with GDM shown significantly higher pre-pregnancy BMI values compared to controls. Moreover, the probability of GDM occurrence significantly increased with increasing HtrA1 levels and BMI values. The ROC curve showed a good accuracy in predicting GDM, with an AUC of 0.74 (95%CI: 0.64–0.92). Conclusions: These results suggest an important role of HtrA1 as an early predictive marker of GDM in the first trimester of gestation, showing a significative clinical relevance for prevention of this disease. |
format | Online Article Text |
id | pubmed-9689498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96894982022-11-25 HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? Tossetta, Giovanni Fantone, Sonia Gesuita, Rosaria Di Renzo, Gian Carlo Meyyazhagan, Arun Tersigni, Chiara Scambia, Giovanni Di Simone, Nicoletta Marzioni, Daniela Diagnostics (Basel) Article Background: The high-temperature requirement A 1 (HtrA1) is a multidomain secretory protein with serine-protease activity, expressed in many tissues, including placenta, where its expression is higher in the first trimester, suggesting an association of this serine protease in early phases of human placenta development. In this study, we evaluated maternal serum HtrA1 levels in the first and third trimester of gestation. In particular, we evaluated a possible role of HtrA1 as an early marker of gestational diabetes mellitus (GDM) in the first trimester of gestation. Methods: We evaluated HtrA1 serum levels in the third trimester (36–40 weeks) in normal pregnancies (n = 20) and GDM pregnancies (n = 20) by using ELISA analysis. Secondly, we performed the same analysis by using the first trimester sera (10–12 weeks) of healthy pregnant women that will develop a normal pregnancy (n = 210) or GDM (n = 28) during pregnancy. Results: We found that HtrA1 serum levels in the third trimester were higher in pregnancies complicated by GDM. Interestingly, higher HtrA1 serum levels were also found in the first trimester in women developing GDM later during the second–third trimester. No significant differences in terms of maternal age and gestational age were found between cases and controls. Women with GDM shown significantly higher pre-pregnancy BMI values compared to controls. Moreover, the probability of GDM occurrence significantly increased with increasing HtrA1 levels and BMI values. The ROC curve showed a good accuracy in predicting GDM, with an AUC of 0.74 (95%CI: 0.64–0.92). Conclusions: These results suggest an important role of HtrA1 as an early predictive marker of GDM in the first trimester of gestation, showing a significative clinical relevance for prevention of this disease. MDPI 2022-11-05 /pmc/articles/PMC9689498/ /pubmed/36359548 http://dx.doi.org/10.3390/diagnostics12112705 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tossetta, Giovanni Fantone, Sonia Gesuita, Rosaria Di Renzo, Gian Carlo Meyyazhagan, Arun Tersigni, Chiara Scambia, Giovanni Di Simone, Nicoletta Marzioni, Daniela HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? |
title | HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? |
title_full | HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? |
title_fullStr | HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? |
title_full_unstemmed | HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? |
title_short | HtrA1 in Gestational Diabetes Mellitus: A Possible Biomarker? |
title_sort | htra1 in gestational diabetes mellitus: a possible biomarker? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689498/ https://www.ncbi.nlm.nih.gov/pubmed/36359548 http://dx.doi.org/10.3390/diagnostics12112705 |
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