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Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways

HIGHLIGHTS: Dietary DHA of 0.42% was beneficial to the growth of common carp fed plant-derived diets; Dietary DHA ameliorated the muscular fatty acid nutrition, and textural firmness of common carp fed plant-derived diets; . Myogenic regulatory factors expression was significantly elevated, while ms...

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Autores principales: He, Zijie, Xu, Chao, Chen, Fang, Lou, Yunkun, Nie, Guoxing, Xie, Dizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689754/
https://www.ncbi.nlm.nih.gov/pubmed/36429191
http://dx.doi.org/10.3390/foods11223600
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author He, Zijie
Xu, Chao
Chen, Fang
Lou, Yunkun
Nie, Guoxing
Xie, Dizhi
author_facet He, Zijie
Xu, Chao
Chen, Fang
Lou, Yunkun
Nie, Guoxing
Xie, Dizhi
author_sort He, Zijie
collection PubMed
description HIGHLIGHTS: Dietary DHA of 0.42% was beneficial to the growth of common carp fed plant-derived diets; Dietary DHA ameliorated the muscular fatty acid nutrition, and textural firmness of common carp fed plant-derived diets; . Myogenic regulatory factors expression was significantly elevated, while mstna expression was decreased by dietary DHA; Dietary DHA modification to the muscular textural firmness of common carp may be attributed to the inhibition of FoxO1 pathways. ABSTRACT: Omega-3 fatty acids have a positive effect on the muscle textural firmness of fish, while the intrinsic mechanism is poorly understood. To investigate the potential mechanism of textural modification caused by dietary docosahexaenoic acid ( DHA) in common carp (Cyprinus carpio L.), three plant-derived diets with varying DHA levels (0%, 0.5%, 1%, D1–D3) were prepared to feed juveniles (initial weight 15.27 ± 0.77 g) for 8 weeks, and the muscular texture, fibers density, and transcriptome were analyzed. The results showed that the growth performance, muscular DHA content, fibers density, and texture of the fish fed diets D2 and D3 were significantly ameliorated compared with the fish fed diet D1. The muscular transcriptome profiles indicated that the up-regulated genes of fish fed dietary DHA mainly in response to muscle proliferation, as well as the FoxO pathway, were significantly enriched in the D2 and D3 groups. Consistent with this, the Quantitative Real-Time PCR (qRT-PCR ) assays indicated that the expression of myogenic regulatory factors (myog, myod, mrf4, mrf5) was up-regulated in the high-DHA groups. Additionally, the expression of foxo1 (inhibitor of myofiber development) mRNA was down-regulated, while its negative regulatory pathway (MAPK and PI3K) was activated in the D2 and D3 groups. The results suggested that the DHA supplementation is beneficial to modifying the muscular textural firmness of common carp fed plant-derived diets, which could be attributed to the inhibition of FoxO1 pathways.
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spelling pubmed-96897542022-11-25 Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways He, Zijie Xu, Chao Chen, Fang Lou, Yunkun Nie, Guoxing Xie, Dizhi Foods Article HIGHLIGHTS: Dietary DHA of 0.42% was beneficial to the growth of common carp fed plant-derived diets; Dietary DHA ameliorated the muscular fatty acid nutrition, and textural firmness of common carp fed plant-derived diets; . Myogenic regulatory factors expression was significantly elevated, while mstna expression was decreased by dietary DHA; Dietary DHA modification to the muscular textural firmness of common carp may be attributed to the inhibition of FoxO1 pathways. ABSTRACT: Omega-3 fatty acids have a positive effect on the muscle textural firmness of fish, while the intrinsic mechanism is poorly understood. To investigate the potential mechanism of textural modification caused by dietary docosahexaenoic acid ( DHA) in common carp (Cyprinus carpio L.), three plant-derived diets with varying DHA levels (0%, 0.5%, 1%, D1–D3) were prepared to feed juveniles (initial weight 15.27 ± 0.77 g) for 8 weeks, and the muscular texture, fibers density, and transcriptome were analyzed. The results showed that the growth performance, muscular DHA content, fibers density, and texture of the fish fed diets D2 and D3 were significantly ameliorated compared with the fish fed diet D1. The muscular transcriptome profiles indicated that the up-regulated genes of fish fed dietary DHA mainly in response to muscle proliferation, as well as the FoxO pathway, were significantly enriched in the D2 and D3 groups. Consistent with this, the Quantitative Real-Time PCR (qRT-PCR ) assays indicated that the expression of myogenic regulatory factors (myog, myod, mrf4, mrf5) was up-regulated in the high-DHA groups. Additionally, the expression of foxo1 (inhibitor of myofiber development) mRNA was down-regulated, while its negative regulatory pathway (MAPK and PI3K) was activated in the D2 and D3 groups. The results suggested that the DHA supplementation is beneficial to modifying the muscular textural firmness of common carp fed plant-derived diets, which could be attributed to the inhibition of FoxO1 pathways. MDPI 2022-11-11 /pmc/articles/PMC9689754/ /pubmed/36429191 http://dx.doi.org/10.3390/foods11223600 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Zijie
Xu, Chao
Chen, Fang
Lou, Yunkun
Nie, Guoxing
Xie, Dizhi
Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways
title Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways
title_full Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways
title_fullStr Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways
title_full_unstemmed Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways
title_short Dietary DHA Enhanced the Textural Firmness of Common Carp (Cyprinus carpio L.) Fed Plant-Derived Diets through Restraining FoxO1 Pathways
title_sort dietary dha enhanced the textural firmness of common carp (cyprinus carpio l.) fed plant-derived diets through restraining foxo1 pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689754/
https://www.ncbi.nlm.nih.gov/pubmed/36429191
http://dx.doi.org/10.3390/foods11223600
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