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RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)

Cyclin B (CycB) plays essential roles in cell proliferation and promotes gonad development in many crustaceans. The goal of this study was to investigate the regulatory roles of this gene in the reproductive development of male oriental river prawns (Macrobrachium nipponense). A phylo-genetic tree a...

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Autores principales: Zhang, Wenyi, Wang, Pengchao, Xiong, Yiwei, Chen, Tianyong, Jiang, Sufei, Qiao, Hui, Gong, Yongsheng, Wu, Yan, Jin, Shubo, Fu, Hongtuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690022/
https://www.ncbi.nlm.nih.gov/pubmed/36360319
http://dx.doi.org/10.3390/genes13112079
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author Zhang, Wenyi
Wang, Pengchao
Xiong, Yiwei
Chen, Tianyong
Jiang, Sufei
Qiao, Hui
Gong, Yongsheng
Wu, Yan
Jin, Shubo
Fu, Hongtuo
author_facet Zhang, Wenyi
Wang, Pengchao
Xiong, Yiwei
Chen, Tianyong
Jiang, Sufei
Qiao, Hui
Gong, Yongsheng
Wu, Yan
Jin, Shubo
Fu, Hongtuo
author_sort Zhang, Wenyi
collection PubMed
description Cyclin B (CycB) plays essential roles in cell proliferation and promotes gonad development in many crustaceans. The goal of this study was to investigate the regulatory roles of this gene in the reproductive development of male oriental river prawns (Macrobrachium nipponense). A phylo-genetic tree analysis revealed that the protein sequence of Mn-CycB was most closely related to those of freshwater prawns, whereas the evolutionary distance from crabs was much longer. A quantitative PCR analysis showed that the expression of Mn-CycB was highest in the gonad of both male and female prawns compared to that in other tissues (p < 0.05), indicating that this gene may play essential roles in the regulation of both testis and ovary development in M. nipponense. In males, Mn-CycB expression in the testis and androgenic gland was higher during the reproductive season than during the non-reproductive season (p < 0.05), implying that CycB plays essential roles in the reproductive development of male M. nipponense. An RNA interference analysis revealed that the Mn-insulin-like androgenic gland hormone expression decreased as the Mn-CycB expression decreased, and that few sperm were detected 14 days after the dsCycB treatment, indicating that CycB positively affects testis development in M. nipponense. The results of this study highlight the functions of CycB in M. nipponense, and they can be applied to studies of male reproductive development in other crustacean species.
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spelling pubmed-96900222022-11-25 RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense) Zhang, Wenyi Wang, Pengchao Xiong, Yiwei Chen, Tianyong Jiang, Sufei Qiao, Hui Gong, Yongsheng Wu, Yan Jin, Shubo Fu, Hongtuo Genes (Basel) Article Cyclin B (CycB) plays essential roles in cell proliferation and promotes gonad development in many crustaceans. The goal of this study was to investigate the regulatory roles of this gene in the reproductive development of male oriental river prawns (Macrobrachium nipponense). A phylo-genetic tree analysis revealed that the protein sequence of Mn-CycB was most closely related to those of freshwater prawns, whereas the evolutionary distance from crabs was much longer. A quantitative PCR analysis showed that the expression of Mn-CycB was highest in the gonad of both male and female prawns compared to that in other tissues (p < 0.05), indicating that this gene may play essential roles in the regulation of both testis and ovary development in M. nipponense. In males, Mn-CycB expression in the testis and androgenic gland was higher during the reproductive season than during the non-reproductive season (p < 0.05), implying that CycB plays essential roles in the reproductive development of male M. nipponense. An RNA interference analysis revealed that the Mn-insulin-like androgenic gland hormone expression decreased as the Mn-CycB expression decreased, and that few sperm were detected 14 days after the dsCycB treatment, indicating that CycB positively affects testis development in M. nipponense. The results of this study highlight the functions of CycB in M. nipponense, and they can be applied to studies of male reproductive development in other crustacean species. MDPI 2022-11-09 /pmc/articles/PMC9690022/ /pubmed/36360319 http://dx.doi.org/10.3390/genes13112079 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Wenyi
Wang, Pengchao
Xiong, Yiwei
Chen, Tianyong
Jiang, Sufei
Qiao, Hui
Gong, Yongsheng
Wu, Yan
Jin, Shubo
Fu, Hongtuo
RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)
title RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)
title_full RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)
title_fullStr RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)
title_full_unstemmed RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)
title_short RNA Interference Analysis of the Functions of Cyclin B in Male Reproductive Development of the Oriental River Prawn (Macrobrachium nipponense)
title_sort rna interference analysis of the functions of cyclin b in male reproductive development of the oriental river prawn (macrobrachium nipponense)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690022/
https://www.ncbi.nlm.nih.gov/pubmed/36360319
http://dx.doi.org/10.3390/genes13112079
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