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ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
Hypertension is an independent risk factor for atherosclerosis. However, few models of hypertensive atherosclerosis have been established in medical research. In this study, we crossed the ApoE knockout (ApoE-KO; ApoE(−/−)) atherosclerotic mouse model with the NOS3 knockout (NOS3-KO; NOS3(−/−)) hype...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690224/ https://www.ncbi.nlm.nih.gov/pubmed/36360235 http://dx.doi.org/10.3390/genes13111998 |
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author | Liu, Ke Chen, Bangzhu Zeng, Fanwen Wang, Gang Wu, Xin Liu, Yueshu Li, Guiling Yan, Jiarong Zhang, Shouquan |
author_facet | Liu, Ke Chen, Bangzhu Zeng, Fanwen Wang, Gang Wu, Xin Liu, Yueshu Li, Guiling Yan, Jiarong Zhang, Shouquan |
author_sort | Liu, Ke |
collection | PubMed |
description | Hypertension is an independent risk factor for atherosclerosis. However, few models of hypertensive atherosclerosis have been established in medical research. In this study, we crossed the ApoE knockout (ApoE-KO; ApoE(−/−)) atherosclerotic mouse model with the NOS3 knockout (NOS3-KO; NOS3(−/−)) hypertensive mouse model to establish an ApoE/NOS3 double knockout (ApoE/NOS3-KO; ApoE/NOS3(−/−)) hypertensive atherosclerosis mouse model. We found that ApoE/NOS3(−/−) mice reproduced normally, had a blood pressure of 133.00 ± 3.85 mmHg, and developed hypertensive fundus retinopathy and hypertensive nephropathy. In addition, serum total cholesterol (TC) and low-density lipoprotein (LDL) levels in the blood were abnormally elevated, steatosis was observed in the liver cells, and atherosclerotic lesions were observed in the aortic vessels in ApoE/NOS3(−/−) adult mice. In conclusion, ApoE/NOS3(−/−) adult mice are a satisfactory model of hypertension and atherosclerosis and can be utilized for studies on cardiovascular diseases. |
format | Online Article Text |
id | pubmed-9690224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96902242022-11-25 ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis Liu, Ke Chen, Bangzhu Zeng, Fanwen Wang, Gang Wu, Xin Liu, Yueshu Li, Guiling Yan, Jiarong Zhang, Shouquan Genes (Basel) Article Hypertension is an independent risk factor for atherosclerosis. However, few models of hypertensive atherosclerosis have been established in medical research. In this study, we crossed the ApoE knockout (ApoE-KO; ApoE(−/−)) atherosclerotic mouse model with the NOS3 knockout (NOS3-KO; NOS3(−/−)) hypertensive mouse model to establish an ApoE/NOS3 double knockout (ApoE/NOS3-KO; ApoE/NOS3(−/−)) hypertensive atherosclerosis mouse model. We found that ApoE/NOS3(−/−) mice reproduced normally, had a blood pressure of 133.00 ± 3.85 mmHg, and developed hypertensive fundus retinopathy and hypertensive nephropathy. In addition, serum total cholesterol (TC) and low-density lipoprotein (LDL) levels in the blood were abnormally elevated, steatosis was observed in the liver cells, and atherosclerotic lesions were observed in the aortic vessels in ApoE/NOS3(−/−) adult mice. In conclusion, ApoE/NOS3(−/−) adult mice are a satisfactory model of hypertension and atherosclerosis and can be utilized for studies on cardiovascular diseases. MDPI 2022-11-01 /pmc/articles/PMC9690224/ /pubmed/36360235 http://dx.doi.org/10.3390/genes13111998 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Ke Chen, Bangzhu Zeng, Fanwen Wang, Gang Wu, Xin Liu, Yueshu Li, Guiling Yan, Jiarong Zhang, Shouquan ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis |
title | ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis |
title_full | ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis |
title_fullStr | ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis |
title_full_unstemmed | ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis |
title_short | ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis |
title_sort | apoe/nos3 knockout mice as a novel cardiovascular disease model of hypertension and atherosclerosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690224/ https://www.ncbi.nlm.nih.gov/pubmed/36360235 http://dx.doi.org/10.3390/genes13111998 |
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