Cargando…

ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis

Hypertension is an independent risk factor for atherosclerosis. However, few models of hypertensive atherosclerosis have been established in medical research. In this study, we crossed the ApoE knockout (ApoE-KO; ApoE(−/−)) atherosclerotic mouse model with the NOS3 knockout (NOS3-KO; NOS3(−/−)) hype...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Ke, Chen, Bangzhu, Zeng, Fanwen, Wang, Gang, Wu, Xin, Liu, Yueshu, Li, Guiling, Yan, Jiarong, Zhang, Shouquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690224/
https://www.ncbi.nlm.nih.gov/pubmed/36360235
http://dx.doi.org/10.3390/genes13111998
_version_ 1784836732871507968
author Liu, Ke
Chen, Bangzhu
Zeng, Fanwen
Wang, Gang
Wu, Xin
Liu, Yueshu
Li, Guiling
Yan, Jiarong
Zhang, Shouquan
author_facet Liu, Ke
Chen, Bangzhu
Zeng, Fanwen
Wang, Gang
Wu, Xin
Liu, Yueshu
Li, Guiling
Yan, Jiarong
Zhang, Shouquan
author_sort Liu, Ke
collection PubMed
description Hypertension is an independent risk factor for atherosclerosis. However, few models of hypertensive atherosclerosis have been established in medical research. In this study, we crossed the ApoE knockout (ApoE-KO; ApoE(−/−)) atherosclerotic mouse model with the NOS3 knockout (NOS3-KO; NOS3(−/−)) hypertensive mouse model to establish an ApoE/NOS3 double knockout (ApoE/NOS3-KO; ApoE/NOS3(−/−)) hypertensive atherosclerosis mouse model. We found that ApoE/NOS3(−/−) mice reproduced normally, had a blood pressure of 133.00 ± 3.85 mmHg, and developed hypertensive fundus retinopathy and hypertensive nephropathy. In addition, serum total cholesterol (TC) and low-density lipoprotein (LDL) levels in the blood were abnormally elevated, steatosis was observed in the liver cells, and atherosclerotic lesions were observed in the aortic vessels in ApoE/NOS3(−/−) adult mice. In conclusion, ApoE/NOS3(−/−) adult mice are a satisfactory model of hypertension and atherosclerosis and can be utilized for studies on cardiovascular diseases.
format Online
Article
Text
id pubmed-9690224
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96902242022-11-25 ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis Liu, Ke Chen, Bangzhu Zeng, Fanwen Wang, Gang Wu, Xin Liu, Yueshu Li, Guiling Yan, Jiarong Zhang, Shouquan Genes (Basel) Article Hypertension is an independent risk factor for atherosclerosis. However, few models of hypertensive atherosclerosis have been established in medical research. In this study, we crossed the ApoE knockout (ApoE-KO; ApoE(−/−)) atherosclerotic mouse model with the NOS3 knockout (NOS3-KO; NOS3(−/−)) hypertensive mouse model to establish an ApoE/NOS3 double knockout (ApoE/NOS3-KO; ApoE/NOS3(−/−)) hypertensive atherosclerosis mouse model. We found that ApoE/NOS3(−/−) mice reproduced normally, had a blood pressure of 133.00 ± 3.85 mmHg, and developed hypertensive fundus retinopathy and hypertensive nephropathy. In addition, serum total cholesterol (TC) and low-density lipoprotein (LDL) levels in the blood were abnormally elevated, steatosis was observed in the liver cells, and atherosclerotic lesions were observed in the aortic vessels in ApoE/NOS3(−/−) adult mice. In conclusion, ApoE/NOS3(−/−) adult mice are a satisfactory model of hypertension and atherosclerosis and can be utilized for studies on cardiovascular diseases. MDPI 2022-11-01 /pmc/articles/PMC9690224/ /pubmed/36360235 http://dx.doi.org/10.3390/genes13111998 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Ke
Chen, Bangzhu
Zeng, Fanwen
Wang, Gang
Wu, Xin
Liu, Yueshu
Li, Guiling
Yan, Jiarong
Zhang, Shouquan
ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
title ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
title_full ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
title_fullStr ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
title_full_unstemmed ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
title_short ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis
title_sort apoe/nos3 knockout mice as a novel cardiovascular disease model of hypertension and atherosclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690224/
https://www.ncbi.nlm.nih.gov/pubmed/36360235
http://dx.doi.org/10.3390/genes13111998
work_keys_str_mv AT liuke apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT chenbangzhu apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT zengfanwen apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT wanggang apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT wuxin apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT liuyueshu apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT liguiling apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT yanjiarong apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis
AT zhangshouquan apoenos3knockoutmiceasanovelcardiovasculardiseasemodelofhypertensionandatherosclerosis