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Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the fu...

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Autores principales: Zając, Ewelina, Fabiańska, Monika J., Jędrszczyk, Elżbieta, Skalski, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690351/
https://www.ncbi.nlm.nih.gov/pubmed/36429785
http://dx.doi.org/10.3390/ijerph192215066
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author Zając, Ewelina
Fabiańska, Monika J.
Jędrszczyk, Elżbieta
Skalski, Tomasz
author_facet Zając, Ewelina
Fabiańska, Monika J.
Jędrszczyk, Elżbieta
Skalski, Tomasz
author_sort Zając, Ewelina
collection PubMed
description To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the future of our planet. We studied the potential of γ-poly glutamic acid (PGA) as a natural component of biofilm produced by Bacillus sp. to be used for the decomposition of petroleum products, such as heavy naphtha (N), lubricating oil (O), and grease (G). The study aimed to assess the impact of the use of different concentrations of PGA on the degradation process of various fractions of petroleum hydrocarbons (PH) and its effect on bacterial population growth in harsh conditions of PH contamination. In laboratory conditions, four treatments of PGA with each of the petroleum products (N, O, and G) were tested: PGA(0) (reference), PGA(1) (1% PGA), PGA(1B) (1% PGA with Bacillus licheniformis), and PGA(10) (10% PGA). After 7, 28, 56, and 112 days of the experiment, the percentage yield extraction, hydrocarbon mass loss, geochemical ratios, pH, electrical conductivity, and microorganisms survival were determined. We observed an increase in PH removal, reflected as a higher amount of extraction yield (growing with time and reaching about 11% in G) and loss of hydrocarbon mass (about 4% in O and G) in all treatments of the PGA compared to the reference. The positive degradation impact was intensive until around day 60. The PH removal stimulation by PGA was also reflected by changes in the values of geochemical ratios, which indicated that the highest rate of degradation was at the initial stage of the process. In general, for the stimulation of PH removal, using a lower (1%) concentration of PGA resulted in better performance than a higher concentration (10%). The PH removal facilitated by PGA is related to the anionic homopoliamid structure of the molecule and its action as a surfactant, which leads to the formation of micelles and the gradual release of PH absorbed in the zeolite carrier. Moreover, the protective properties of PGA against the extinction of bacteria under high concentrations of PH were identified. Generally, the γ-PGA biopolymer helps to degrade the hydrocarbon pollutants and stabilize the environment suitable for microbial degraders development.
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spelling pubmed-96903512022-11-25 Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application Zając, Ewelina Fabiańska, Monika J. Jędrszczyk, Elżbieta Skalski, Tomasz Int J Environ Res Public Health Article To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the future of our planet. We studied the potential of γ-poly glutamic acid (PGA) as a natural component of biofilm produced by Bacillus sp. to be used for the decomposition of petroleum products, such as heavy naphtha (N), lubricating oil (O), and grease (G). The study aimed to assess the impact of the use of different concentrations of PGA on the degradation process of various fractions of petroleum hydrocarbons (PH) and its effect on bacterial population growth in harsh conditions of PH contamination. In laboratory conditions, four treatments of PGA with each of the petroleum products (N, O, and G) were tested: PGA(0) (reference), PGA(1) (1% PGA), PGA(1B) (1% PGA with Bacillus licheniformis), and PGA(10) (10% PGA). After 7, 28, 56, and 112 days of the experiment, the percentage yield extraction, hydrocarbon mass loss, geochemical ratios, pH, electrical conductivity, and microorganisms survival were determined. We observed an increase in PH removal, reflected as a higher amount of extraction yield (growing with time and reaching about 11% in G) and loss of hydrocarbon mass (about 4% in O and G) in all treatments of the PGA compared to the reference. The positive degradation impact was intensive until around day 60. The PH removal stimulation by PGA was also reflected by changes in the values of geochemical ratios, which indicated that the highest rate of degradation was at the initial stage of the process. In general, for the stimulation of PH removal, using a lower (1%) concentration of PGA resulted in better performance than a higher concentration (10%). The PH removal facilitated by PGA is related to the anionic homopoliamid structure of the molecule and its action as a surfactant, which leads to the formation of micelles and the gradual release of PH absorbed in the zeolite carrier. Moreover, the protective properties of PGA against the extinction of bacteria under high concentrations of PH were identified. Generally, the γ-PGA biopolymer helps to degrade the hydrocarbon pollutants and stabilize the environment suitable for microbial degraders development. MDPI 2022-11-16 /pmc/articles/PMC9690351/ /pubmed/36429785 http://dx.doi.org/10.3390/ijerph192215066 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zając, Ewelina
Fabiańska, Monika J.
Jędrszczyk, Elżbieta
Skalski, Tomasz
Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application
title Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application
title_full Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application
title_fullStr Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application
title_full_unstemmed Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application
title_short Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application
title_sort hydrocarbon degradation and microbial survival improvement in response to γ-polyglutamic acid application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690351/
https://www.ncbi.nlm.nih.gov/pubmed/36429785
http://dx.doi.org/10.3390/ijerph192215066
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