Cargando…

Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)

Insect mitochondrial genomes (mitogenomes) are of great interest in exploring molecular evolution, phylogenetics, and biogeography. So far, only 12 mitogenomes of the leafhopper tribe Idiocerini have been released in GenBank, although the tribe comprises 488 known species including some agricultural...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Lili, Yang, Wenxin, Si, Chengyan, Guo, Xianguang, Zhang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690763/
https://www.ncbi.nlm.nih.gov/pubmed/36360236
http://dx.doi.org/10.3390/genes13112000
_version_ 1784836873935388672
author Tian, Lili
Yang, Wenxin
Si, Chengyan
Guo, Xianguang
Zhang, Bin
author_facet Tian, Lili
Yang, Wenxin
Si, Chengyan
Guo, Xianguang
Zhang, Bin
author_sort Tian, Lili
collection PubMed
description Insect mitochondrial genomes (mitogenomes) are of great interest in exploring molecular evolution, phylogenetics, and biogeography. So far, only 12 mitogenomes of the leafhopper tribe Idiocerini have been released in GenBank, although the tribe comprises 488 known species including some agricultural, forestry, and horticultural pests. In order to compare and analyze the mitochondrial genome structure of Idiocerini and even the selective pressure of 13 protein-coding genes (PCGs) of the family Cicadellidae, the complete mitogenomes of five species including Nabicerus dentimus, Sahlbergotettix salicicola, Podulmorinus opacus, Podulmorinus consimilis, and a new species of a new genus were determined by next-generation sequencing. The size of the newly determined mitogenomes ranged from 14,733 bp to 15,044 bp, comprising the standard set of 13 PCGs, 22 transfer RNA genes, two ribosomal RNA genes, and a long non-coding control region (CR). The extent of purifying selection presented different pictures in the tribe and the family. The less pronounced genes (0.5 < dN/dS < 1) were nad5 and nad4l in Idiocerin, whereas in the family Cicadellidae including the sequences of Idiocerin, nad1-nad6 and cox1 genes were less pronounced. The codon encoding leucine was the most common in all species, and the codon encoding serine 1 was the most common in all species except for P. opacus. Interestingly, in P. opacus, another of the most common codons is that encoding serine 2. Among the 17 examined species of the Idiocerini, 14 species contained the tandem repeats, and 11 species of them contained the motif “TTATA”. These findings will promote research on the structure and evolution of the mitochondrial genome and highlight the need for more mitogenomes in Cicadellidae.
format Online
Article
Text
id pubmed-9690763
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96907632022-11-25 Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae) Tian, Lili Yang, Wenxin Si, Chengyan Guo, Xianguang Zhang, Bin Genes (Basel) Article Insect mitochondrial genomes (mitogenomes) are of great interest in exploring molecular evolution, phylogenetics, and biogeography. So far, only 12 mitogenomes of the leafhopper tribe Idiocerini have been released in GenBank, although the tribe comprises 488 known species including some agricultural, forestry, and horticultural pests. In order to compare and analyze the mitochondrial genome structure of Idiocerini and even the selective pressure of 13 protein-coding genes (PCGs) of the family Cicadellidae, the complete mitogenomes of five species including Nabicerus dentimus, Sahlbergotettix salicicola, Podulmorinus opacus, Podulmorinus consimilis, and a new species of a new genus were determined by next-generation sequencing. The size of the newly determined mitogenomes ranged from 14,733 bp to 15,044 bp, comprising the standard set of 13 PCGs, 22 transfer RNA genes, two ribosomal RNA genes, and a long non-coding control region (CR). The extent of purifying selection presented different pictures in the tribe and the family. The less pronounced genes (0.5 < dN/dS < 1) were nad5 and nad4l in Idiocerin, whereas in the family Cicadellidae including the sequences of Idiocerin, nad1-nad6 and cox1 genes were less pronounced. The codon encoding leucine was the most common in all species, and the codon encoding serine 1 was the most common in all species except for P. opacus. Interestingly, in P. opacus, another of the most common codons is that encoding serine 2. Among the 17 examined species of the Idiocerini, 14 species contained the tandem repeats, and 11 species of them contained the motif “TTATA”. These findings will promote research on the structure and evolution of the mitochondrial genome and highlight the need for more mitogenomes in Cicadellidae. MDPI 2022-11-01 /pmc/articles/PMC9690763/ /pubmed/36360236 http://dx.doi.org/10.3390/genes13112000 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tian, Lili
Yang, Wenxin
Si, Chengyan
Guo, Xianguang
Zhang, Bin
Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)
title Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)
title_full Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)
title_fullStr Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)
title_full_unstemmed Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)
title_short Complete Mitogenome Analysis of Five Leafhopper Species of Idiocerini (Hemiptera: Cicadellidae)
title_sort complete mitogenome analysis of five leafhopper species of idiocerini (hemiptera: cicadellidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690763/
https://www.ncbi.nlm.nih.gov/pubmed/36360236
http://dx.doi.org/10.3390/genes13112000
work_keys_str_mv AT tianlili completemitogenomeanalysisoffiveleafhopperspeciesofidiocerinihemipteracicadellidae
AT yangwenxin completemitogenomeanalysisoffiveleafhopperspeciesofidiocerinihemipteracicadellidae
AT sichengyan completemitogenomeanalysisoffiveleafhopperspeciesofidiocerinihemipteracicadellidae
AT guoxianguang completemitogenomeanalysisoffiveleafhopperspeciesofidiocerinihemipteracicadellidae
AT zhangbin completemitogenomeanalysisoffiveleafhopperspeciesofidiocerinihemipteracicadellidae