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Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs

The intuitive impression of pork is extremely important in terms of whether consumers are enthusiastic about purchasing it. Flesh color and intramuscular fat (IMF) are indispensable indicators in meat quality assessment. In this study, we determined the flesh color and intramuscular fat at 45 min an...

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Autores principales: Li, Hao, Xu, Cineng, Meng, Fanming, Yao, Zekai, Fan, Zhenfei, Yang, Yingshan, Meng, Xianglun, Zhan, Yuexin, Sun, Ying, Ma, Fucai, Yang, Jifei, Yang, Ming, Yang, Jie, Wu, Zhenfang, Cai, Gengyuan, Zheng, Enqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690869/
https://www.ncbi.nlm.nih.gov/pubmed/36421806
http://dx.doi.org/10.3390/genes13112131
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author Li, Hao
Xu, Cineng
Meng, Fanming
Yao, Zekai
Fan, Zhenfei
Yang, Yingshan
Meng, Xianglun
Zhan, Yuexin
Sun, Ying
Ma, Fucai
Yang, Jifei
Yang, Ming
Yang, Jie
Wu, Zhenfang
Cai, Gengyuan
Zheng, Enqin
author_facet Li, Hao
Xu, Cineng
Meng, Fanming
Yao, Zekai
Fan, Zhenfei
Yang, Yingshan
Meng, Xianglun
Zhan, Yuexin
Sun, Ying
Ma, Fucai
Yang, Jifei
Yang, Ming
Yang, Jie
Wu, Zhenfang
Cai, Gengyuan
Zheng, Enqin
author_sort Li, Hao
collection PubMed
description The intuitive impression of pork is extremely important in terms of whether consumers are enthusiastic about purchasing it. Flesh color and intramuscular fat (IMF) are indispensable indicators in meat quality assessment. In this study, we determined the flesh color and intramuscular fat at 45 min and 12 h after slaughter (45 mFC, 45 mIMF, 12 hFC, and 12 hIMF) of 1518 commercial Duroc × Landrace × Large White (DLY) pigs. We performed a single nucleotide polymorphism (SNP) genome-wide association study (GWAS) analysis with 28,066 SNPs. This experiment found that the correlation between 45 mFC and 12 hFC was 0.343. The correlation between 45 mIMF and 12 hIMF was 0.238. The heritability of the traits 45 mFC, 12 hFC, 45 mIMF, and 12 hIMF was 0.112, 0.217, 0.139, and 0.178, respectively, and we identified seven SNPs for flesh color and three SNPs for IMF. Finally, several candidate genes regulating these four traits were identified. Three candidate genes related to flesh color were provided: SNCAIP and PRR16 on SSC2, ST3GAL4 on SSC5, and GALR1 on SSC1. A total of three candidate genes related to intramuscular fat were found, including ABLIM3 on SSC2, DPH5 on SSC4, and DOCK10 on SSC15. Furthermore, GO and KEGG analysis revealed that these genes are involved in the regulation of apoptosis and are implicated in functions such as pigmentation and skeletal muscle metabolism. This study applied GWAS to analyze the scoring results of flesh color and IMF in different time periods, and it further revealed the genetic structure of flesh color and IMF traits, which may provide important genetic loci for the subsequent improvement of pig meat quality traits.
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spelling pubmed-96908692022-11-25 Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs Li, Hao Xu, Cineng Meng, Fanming Yao, Zekai Fan, Zhenfei Yang, Yingshan Meng, Xianglun Zhan, Yuexin Sun, Ying Ma, Fucai Yang, Jifei Yang, Ming Yang, Jie Wu, Zhenfang Cai, Gengyuan Zheng, Enqin Genes (Basel) Article The intuitive impression of pork is extremely important in terms of whether consumers are enthusiastic about purchasing it. Flesh color and intramuscular fat (IMF) are indispensable indicators in meat quality assessment. In this study, we determined the flesh color and intramuscular fat at 45 min and 12 h after slaughter (45 mFC, 45 mIMF, 12 hFC, and 12 hIMF) of 1518 commercial Duroc × Landrace × Large White (DLY) pigs. We performed a single nucleotide polymorphism (SNP) genome-wide association study (GWAS) analysis with 28,066 SNPs. This experiment found that the correlation between 45 mFC and 12 hFC was 0.343. The correlation between 45 mIMF and 12 hIMF was 0.238. The heritability of the traits 45 mFC, 12 hFC, 45 mIMF, and 12 hIMF was 0.112, 0.217, 0.139, and 0.178, respectively, and we identified seven SNPs for flesh color and three SNPs for IMF. Finally, several candidate genes regulating these four traits were identified. Three candidate genes related to flesh color were provided: SNCAIP and PRR16 on SSC2, ST3GAL4 on SSC5, and GALR1 on SSC1. A total of three candidate genes related to intramuscular fat were found, including ABLIM3 on SSC2, DPH5 on SSC4, and DOCK10 on SSC15. Furthermore, GO and KEGG analysis revealed that these genes are involved in the regulation of apoptosis and are implicated in functions such as pigmentation and skeletal muscle metabolism. This study applied GWAS to analyze the scoring results of flesh color and IMF in different time periods, and it further revealed the genetic structure of flesh color and IMF traits, which may provide important genetic loci for the subsequent improvement of pig meat quality traits. MDPI 2022-11-16 /pmc/articles/PMC9690869/ /pubmed/36421806 http://dx.doi.org/10.3390/genes13112131 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Hao
Xu, Cineng
Meng, Fanming
Yao, Zekai
Fan, Zhenfei
Yang, Yingshan
Meng, Xianglun
Zhan, Yuexin
Sun, Ying
Ma, Fucai
Yang, Jifei
Yang, Ming
Yang, Jie
Wu, Zhenfang
Cai, Gengyuan
Zheng, Enqin
Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs
title Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs
title_full Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs
title_fullStr Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs
title_full_unstemmed Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs
title_short Genome-Wide Association Studies for Flesh Color and Intramuscular Fat in (Duroc × Landrace × Large White) Crossbred Commercial Pigs
title_sort genome-wide association studies for flesh color and intramuscular fat in (duroc × landrace × large white) crossbred commercial pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9690869/
https://www.ncbi.nlm.nih.gov/pubmed/36421806
http://dx.doi.org/10.3390/genes13112131
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