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Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey

An increase in the number of vertebrae can significantly affect the meat production performance of livestock, thus increasing carcass weight, which is of great importance for livestock production. The homeobox gene C8 (HOXC8) has been identified as an essential candidate gene for regulating vertebra...

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Autores principales: Shi, Xiaoyuan, Li, Yan, Wang, Tianqi, Ren, Wei, Huang, Bingjian, Wang, Xinrui, Liu, Ziwen, Liang, Huili, Kou, Xiyan, Chen, Yinghui, Wang, Yonghui, Akhtar, Faheem, Wang, Changfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691153/
https://www.ncbi.nlm.nih.gov/pubmed/36421849
http://dx.doi.org/10.3390/genes13112175
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author Shi, Xiaoyuan
Li, Yan
Wang, Tianqi
Ren, Wei
Huang, Bingjian
Wang, Xinrui
Liu, Ziwen
Liang, Huili
Kou, Xiyan
Chen, Yinghui
Wang, Yonghui
Akhtar, Faheem
Wang, Changfa
author_facet Shi, Xiaoyuan
Li, Yan
Wang, Tianqi
Ren, Wei
Huang, Bingjian
Wang, Xinrui
Liu, Ziwen
Liang, Huili
Kou, Xiyan
Chen, Yinghui
Wang, Yonghui
Akhtar, Faheem
Wang, Changfa
author_sort Shi, Xiaoyuan
collection PubMed
description An increase in the number of vertebrae can significantly affect the meat production performance of livestock, thus increasing carcass weight, which is of great importance for livestock production. The homeobox gene C8 (HOXC8) has been identified as an essential candidate gene for regulating vertebral development. However, it has not been researched on the Dezhou donkey. This study aimed to verify the Dezhou donkey HOXC8 gene’s polymorphisms and assess their effects on multiple vertebral numbers and carcass weight. In this study, the entire HOXC8 gene of the Dezhou donkey was sequenced, SNPs at the whole gene level were identified, and typing was accomplished utilizing a targeted sequencing genotype detection technique (GBTS). Then, a general linear model was used to perform an association study of HOXC8 gene polymorphism loci, multiple vertebral numbers, and carcass weight for screening candidate markers that can be used for molecular breeding of Dezhou donkeys. These findings revealed that HOXC8 included 12 SNPs, all unique mutant loci. The HOXC8 g.15179224C>T was significantly negatively associated with carcass weight (CW) and lumbar vertebrae length (LL) (p < 0.05). The g.15179674G>A locus was shown to be significantly positively associated with the number of lumbar vertebrae (LN) (p < 0.05). The phylogenetic tree constructed for the Dezhou donkey HOXC8 gene and seven other species revealed that the HOXC8 gene was highly conserved during animal evolution but differed markedly among distantly related animals. The results suggest that HOXC8 is a vital gene affecting multiple vertebral numbers and carcass weight in Dezhou donkeys, and the two loci g.15179224C>T and g.15179674G>A may be potential genetic markers for screening and breeding of new strains of high-quality and high-yielding Dezhou donkeys.
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spelling pubmed-96911532022-11-25 Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey Shi, Xiaoyuan Li, Yan Wang, Tianqi Ren, Wei Huang, Bingjian Wang, Xinrui Liu, Ziwen Liang, Huili Kou, Xiyan Chen, Yinghui Wang, Yonghui Akhtar, Faheem Wang, Changfa Genes (Basel) Article An increase in the number of vertebrae can significantly affect the meat production performance of livestock, thus increasing carcass weight, which is of great importance for livestock production. The homeobox gene C8 (HOXC8) has been identified as an essential candidate gene for regulating vertebral development. However, it has not been researched on the Dezhou donkey. This study aimed to verify the Dezhou donkey HOXC8 gene’s polymorphisms and assess their effects on multiple vertebral numbers and carcass weight. In this study, the entire HOXC8 gene of the Dezhou donkey was sequenced, SNPs at the whole gene level were identified, and typing was accomplished utilizing a targeted sequencing genotype detection technique (GBTS). Then, a general linear model was used to perform an association study of HOXC8 gene polymorphism loci, multiple vertebral numbers, and carcass weight for screening candidate markers that can be used for molecular breeding of Dezhou donkeys. These findings revealed that HOXC8 included 12 SNPs, all unique mutant loci. The HOXC8 g.15179224C>T was significantly negatively associated with carcass weight (CW) and lumbar vertebrae length (LL) (p < 0.05). The g.15179674G>A locus was shown to be significantly positively associated with the number of lumbar vertebrae (LN) (p < 0.05). The phylogenetic tree constructed for the Dezhou donkey HOXC8 gene and seven other species revealed that the HOXC8 gene was highly conserved during animal evolution but differed markedly among distantly related animals. The results suggest that HOXC8 is a vital gene affecting multiple vertebral numbers and carcass weight in Dezhou donkeys, and the two loci g.15179224C>T and g.15179674G>A may be potential genetic markers for screening and breeding of new strains of high-quality and high-yielding Dezhou donkeys. MDPI 2022-11-21 /pmc/articles/PMC9691153/ /pubmed/36421849 http://dx.doi.org/10.3390/genes13112175 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Xiaoyuan
Li, Yan
Wang, Tianqi
Ren, Wei
Huang, Bingjian
Wang, Xinrui
Liu, Ziwen
Liang, Huili
Kou, Xiyan
Chen, Yinghui
Wang, Yonghui
Akhtar, Faheem
Wang, Changfa
Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey
title Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey
title_full Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey
title_fullStr Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey
title_full_unstemmed Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey
title_short Association of HOXC8 Genetic Polymorphisms with Multi-Vertebral Number and Carcass Weight in Dezhou Donkey
title_sort association of hoxc8 genetic polymorphisms with multi-vertebral number and carcass weight in dezhou donkey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691153/
https://www.ncbi.nlm.nih.gov/pubmed/36421849
http://dx.doi.org/10.3390/genes13112175
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