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Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity

AhFAD2 is a key enzyme catalyzing the conversion of oleic acid into linoleic acid. The high oleic acid characteristic of peanut mainly comes from the homozygous recessive mutation of AhFAD2A and AhFAD2B genes (aabb). However, even in high-oleic-acid varieties with the aabb genotype, the oleic acid c...

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Autores principales: Zhao, Shuzhen, Sun, Jie, Sun, Jinbo, Zhang, Xiaoqian, Zhao, Chuanzhi, Pan, Jiaowen, Hou, Lei, Tian, Ruizheng, Wang, Xingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691258/
https://www.ncbi.nlm.nih.gov/pubmed/36360313
http://dx.doi.org/10.3390/genes13112076
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author Zhao, Shuzhen
Sun, Jie
Sun, Jinbo
Zhang, Xiaoqian
Zhao, Chuanzhi
Pan, Jiaowen
Hou, Lei
Tian, Ruizheng
Wang, Xingjun
author_facet Zhao, Shuzhen
Sun, Jie
Sun, Jinbo
Zhang, Xiaoqian
Zhao, Chuanzhi
Pan, Jiaowen
Hou, Lei
Tian, Ruizheng
Wang, Xingjun
author_sort Zhao, Shuzhen
collection PubMed
description AhFAD2 is a key enzyme catalyzing the conversion of oleic acid into linoleic acid. The high oleic acid characteristic of peanut mainly comes from the homozygous recessive mutation of AhFAD2A and AhFAD2B genes (aabb). However, even in high-oleic-acid varieties with the aabb genotype, the oleic acid content of seeds with different maturity varies significantly. Therefore, in addition to AhFAD2A and AhFAD2B, other FAD2 members or regulators may be involved in this process. Which FAD2 genes are involved in the regulatory processes associated with seed maturity is still unclear. In this study, four stable lines with different genotypes (AABB, aaBB, AAbb, and aabb) were used to analyze the contents of oleic acid and linoleic acid at different stages of seed development in peanut. Three new AhFAD2 genes (AhFAD2–7, AhFAD2–8, and AhFAD2–9) were cloned based on the whole-genome sequencing results of cultivated peanuts. All peanut FAD2 genes showed tissue preference in expression; however, only the expression level of AhFAD2-7 was positively correlated with the linoleic acid concentration in peanut seeds. These findings provide new insights into the regulation of oleic acid accumulation by maturity, and AhFAD2-7 plays an important role in the maturity dependent accumulation of oleic acid and linoleic acid in peanut.
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spelling pubmed-96912582022-11-25 Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity Zhao, Shuzhen Sun, Jie Sun, Jinbo Zhang, Xiaoqian Zhao, Chuanzhi Pan, Jiaowen Hou, Lei Tian, Ruizheng Wang, Xingjun Genes (Basel) Article AhFAD2 is a key enzyme catalyzing the conversion of oleic acid into linoleic acid. The high oleic acid characteristic of peanut mainly comes from the homozygous recessive mutation of AhFAD2A and AhFAD2B genes (aabb). However, even in high-oleic-acid varieties with the aabb genotype, the oleic acid content of seeds with different maturity varies significantly. Therefore, in addition to AhFAD2A and AhFAD2B, other FAD2 members or regulators may be involved in this process. Which FAD2 genes are involved in the regulatory processes associated with seed maturity is still unclear. In this study, four stable lines with different genotypes (AABB, aaBB, AAbb, and aabb) were used to analyze the contents of oleic acid and linoleic acid at different stages of seed development in peanut. Three new AhFAD2 genes (AhFAD2–7, AhFAD2–8, and AhFAD2–9) were cloned based on the whole-genome sequencing results of cultivated peanuts. All peanut FAD2 genes showed tissue preference in expression; however, only the expression level of AhFAD2-7 was positively correlated with the linoleic acid concentration in peanut seeds. These findings provide new insights into the regulation of oleic acid accumulation by maturity, and AhFAD2-7 plays an important role in the maturity dependent accumulation of oleic acid and linoleic acid in peanut. MDPI 2022-11-09 /pmc/articles/PMC9691258/ /pubmed/36360313 http://dx.doi.org/10.3390/genes13112076 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Shuzhen
Sun, Jie
Sun, Jinbo
Zhang, Xiaoqian
Zhao, Chuanzhi
Pan, Jiaowen
Hou, Lei
Tian, Ruizheng
Wang, Xingjun
Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity
title Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity
title_full Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity
title_fullStr Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity
title_full_unstemmed Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity
title_short Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity
title_sort insights into the novel fad2 gene regulating oleic acid accumulation in peanut seeds with different maturity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691258/
https://www.ncbi.nlm.nih.gov/pubmed/36360313
http://dx.doi.org/10.3390/genes13112076
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