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Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass
The production of extremozymes from halophilic bacteria has increased significantly due to their stability and efficiency in catalyzing a reaction, as well as their capacity to display optimum activity at various salt concentrations. In the current study, the halophilic bacterium Virgibacillus salar...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691493/ https://www.ncbi.nlm.nih.gov/pubmed/36422734 http://dx.doi.org/10.1007/s11274-022-03446-7 |
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author | Yousef, Naeima M. H. Mawad, Asmaa M. M. |
author_facet | Yousef, Naeima M. H. Mawad, Asmaa M. M. |
author_sort | Yousef, Naeima M. H. |
collection | PubMed |
description | The production of extremozymes from halophilic bacteria has increased significantly due to their stability and efficiency in catalyzing a reaction, as well as their capacity to display optimum activity at various salt concentrations. In the current study, the halophilic bacterium Virgibacillus salarius strain BM-02 could utilize many non-pretreated substrates including cellulose, corn stover, sugarcane bagasse and wheat bran as a sole carbon source. However, wheat bran was the best substrate for achieving optimum saccharification yield (90.1%). The partially purified cellulase was active and stable at a wide range of pH (5–8) with residual activities > 58%. Moreover, it was stable at 5–12% of NaCl. Metal ions have a variable impact on the activity of partially purified cellulase however, Fe(+3) exhibited the highest increase in the cellulase activity. The enzyme exhibited a thermal stability at 40, 50 and 60 °C with half-lives of 1049.50, 168.14 and 163.5 min, respectively. The value of V(max) was 22.27 U/mL while Km was 2.1 mM. The activation energy of denaturation E(d) 69.81 kJ/mol, the enthalpy values (ΔH(d)) were positive, and the entropy values (ΔS) were negative. Therefore, V. Salarius is recommended as a novel promising halophilic extremozyme producer and agricultural waste remover in the bio-industrial applications. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-9691493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-96914932022-11-26 Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass Yousef, Naeima M. H. Mawad, Asmaa M. M. World J Microbiol Biotechnol Research The production of extremozymes from halophilic bacteria has increased significantly due to their stability and efficiency in catalyzing a reaction, as well as their capacity to display optimum activity at various salt concentrations. In the current study, the halophilic bacterium Virgibacillus salarius strain BM-02 could utilize many non-pretreated substrates including cellulose, corn stover, sugarcane bagasse and wheat bran as a sole carbon source. However, wheat bran was the best substrate for achieving optimum saccharification yield (90.1%). The partially purified cellulase was active and stable at a wide range of pH (5–8) with residual activities > 58%. Moreover, it was stable at 5–12% of NaCl. Metal ions have a variable impact on the activity of partially purified cellulase however, Fe(+3) exhibited the highest increase in the cellulase activity. The enzyme exhibited a thermal stability at 40, 50 and 60 °C with half-lives of 1049.50, 168.14 and 163.5 min, respectively. The value of V(max) was 22.27 U/mL while Km was 2.1 mM. The activation energy of denaturation E(d) 69.81 kJ/mol, the enthalpy values (ΔH(d)) were positive, and the entropy values (ΔS) were negative. Therefore, V. Salarius is recommended as a novel promising halophilic extremozyme producer and agricultural waste remover in the bio-industrial applications. GRAPHICAL ABSTRACT: [Image: see text] Springer Netherlands 2022-11-24 2023 /pmc/articles/PMC9691493/ /pubmed/36422734 http://dx.doi.org/10.1007/s11274-022-03446-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Yousef, Naeima M. H. Mawad, Asmaa M. M. Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass |
title | Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass |
title_full | Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass |
title_fullStr | Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass |
title_full_unstemmed | Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass |
title_short | Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass |
title_sort | characterization of thermo/halo stable cellulase produced from halophilic virgibacillus salarius bm-02 using non-pretreated biomass |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691493/ https://www.ncbi.nlm.nih.gov/pubmed/36422734 http://dx.doi.org/10.1007/s11274-022-03446-7 |
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