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Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish

Of the three primary cannabinoids in cannabis: Δ(9)-Tetrahydrocannabinol (Δ(9)-THC), cannabidiol (CBD) and cannabinol (CBN), very little is known about the actions of CBN, the primary oxidative metabolite of THC. Our goal was to determine if CBN exposure during gastrulation alters embryonic developm...

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Autores principales: Amin, Md Ruhul, Ahmed, Kazi Tanveer, Ali, Declan William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691751/
https://www.ncbi.nlm.nih.gov/pubmed/36424484
http://dx.doi.org/10.1038/s41598-022-23495-0
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author Amin, Md Ruhul
Ahmed, Kazi Tanveer
Ali, Declan William
author_facet Amin, Md Ruhul
Ahmed, Kazi Tanveer
Ali, Declan William
author_sort Amin, Md Ruhul
collection PubMed
description Of the three primary cannabinoids in cannabis: Δ(9)-Tetrahydrocannabinol (Δ(9)-THC), cannabidiol (CBD) and cannabinol (CBN), very little is known about the actions of CBN, the primary oxidative metabolite of THC. Our goal was to determine if CBN exposure during gastrulation alters embryonic development, and if so, does it act via the canonical cannabinoid receptors. Zebrafish embryos were exposed to CBN during gastrulation and exhibited dose-dependent malformations, increased mortality, decreased locomotion and a reduction in motor neuron branching. Moreover, larva showed a significant reduction in the response to sound stimuli. CBN exposure altered the development of hair cells associated with otic vesicles and the lateral line. Pharmacological block of Cb2rs with AM 630 or JTE 907 prevented many of the CBN-induced developmental defects, while block of Cb1rs with AM 251 or CP 945598 had little or no effect. Altogether we show that embryonic exposure to CBN results in alterations in embryonic growth, neuronal and hair cell development, physiology and behavior via Cb2r-mediated mechanisms.
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spelling pubmed-96917512022-11-26 Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish Amin, Md Ruhul Ahmed, Kazi Tanveer Ali, Declan William Sci Rep Article Of the three primary cannabinoids in cannabis: Δ(9)-Tetrahydrocannabinol (Δ(9)-THC), cannabidiol (CBD) and cannabinol (CBN), very little is known about the actions of CBN, the primary oxidative metabolite of THC. Our goal was to determine if CBN exposure during gastrulation alters embryonic development, and if so, does it act via the canonical cannabinoid receptors. Zebrafish embryos were exposed to CBN during gastrulation and exhibited dose-dependent malformations, increased mortality, decreased locomotion and a reduction in motor neuron branching. Moreover, larva showed a significant reduction in the response to sound stimuli. CBN exposure altered the development of hair cells associated with otic vesicles and the lateral line. Pharmacological block of Cb2rs with AM 630 or JTE 907 prevented many of the CBN-induced developmental defects, while block of Cb1rs with AM 251 or CP 945598 had little or no effect. Altogether we show that embryonic exposure to CBN results in alterations in embryonic growth, neuronal and hair cell development, physiology and behavior via Cb2r-mediated mechanisms. Nature Publishing Group UK 2022-11-24 /pmc/articles/PMC9691751/ /pubmed/36424484 http://dx.doi.org/10.1038/s41598-022-23495-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Amin, Md Ruhul
Ahmed, Kazi Tanveer
Ali, Declan William
Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish
title Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish
title_full Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish
title_fullStr Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish
title_full_unstemmed Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish
title_short Cannabinoid receptor 2 (Cb2r) mediates cannabinol (CBN) induced developmental defects in zebrafish
title_sort cannabinoid receptor 2 (cb2r) mediates cannabinol (cbn) induced developmental defects in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691751/
https://www.ncbi.nlm.nih.gov/pubmed/36424484
http://dx.doi.org/10.1038/s41598-022-23495-0
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