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Ephrin-B2-expressing natural killer cells induce angiogenesis

BACKGROUND: Therapeutic angiogenesis aims to induce new blood vessel growth in ischemic tissues; however, previous clinical trials have had limited success. Studies of uterine angiogenesis revealed a specialized subset of natural killer (NK) cells, called uterine NK (uNK) cells, which have unique pr...

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Detalles Bibliográficos
Autores principales: Wolf, Katharine G., Crawford, Emily B., Wartan, Nora M., Schneiderman, Sylvia K., Riehl, Valerie E., Dambaeva, Svetlana V., Beaman, Kenneth D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692026/
https://www.ncbi.nlm.nih.gov/pubmed/36439700
http://dx.doi.org/10.1016/j.jvssci.2022.08.003
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author Wolf, Katharine G.
Crawford, Emily B.
Wartan, Nora M.
Schneiderman, Sylvia K.
Riehl, Valerie E.
Dambaeva, Svetlana V.
Beaman, Kenneth D.
author_facet Wolf, Katharine G.
Crawford, Emily B.
Wartan, Nora M.
Schneiderman, Sylvia K.
Riehl, Valerie E.
Dambaeva, Svetlana V.
Beaman, Kenneth D.
author_sort Wolf, Katharine G.
collection PubMed
description BACKGROUND: Therapeutic angiogenesis aims to induce new blood vessel growth in ischemic tissues; however, previous clinical trials have had limited success. Studies of uterine angiogenesis revealed a specialized subset of natural killer (NK) cells, called uterine NK (uNK) cells, which have unique proangiogenic abilities. METHODS: We show that uNK cells in mice express ephrin-B2, a regulator of angiogenesis, to induce tubule formation in an ex vivo coculture tubule formation assay. We next induced the expression of ephrin-B2 by splenic NK (sNK) cells harvested from male mice. RESULTS: We showed that induced NK (iNK) cells can also instruct endothelial cells to form tubules using ephrin-B2. CONCLUSIONS: We concluded that Ephrin-B2 is a marker of proangiogenic uNK cells and that a proangiogenic phenotype characterized by ephrin-B2 can be induced in sNK cells to induce therapeutic angiogenesis.
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spelling pubmed-96920262022-11-26 Ephrin-B2-expressing natural killer cells induce angiogenesis Wolf, Katharine G. Crawford, Emily B. Wartan, Nora M. Schneiderman, Sylvia K. Riehl, Valerie E. Dambaeva, Svetlana V. Beaman, Kenneth D. JVS Vasc Sci Article BACKGROUND: Therapeutic angiogenesis aims to induce new blood vessel growth in ischemic tissues; however, previous clinical trials have had limited success. Studies of uterine angiogenesis revealed a specialized subset of natural killer (NK) cells, called uterine NK (uNK) cells, which have unique proangiogenic abilities. METHODS: We show that uNK cells in mice express ephrin-B2, a regulator of angiogenesis, to induce tubule formation in an ex vivo coculture tubule formation assay. We next induced the expression of ephrin-B2 by splenic NK (sNK) cells harvested from male mice. RESULTS: We showed that induced NK (iNK) cells can also instruct endothelial cells to form tubules using ephrin-B2. CONCLUSIONS: We concluded that Ephrin-B2 is a marker of proangiogenic uNK cells and that a proangiogenic phenotype characterized by ephrin-B2 can be induced in sNK cells to induce therapeutic angiogenesis. Elsevier 2022-10-27 /pmc/articles/PMC9692026/ /pubmed/36439700 http://dx.doi.org/10.1016/j.jvssci.2022.08.003 Text en Copyright © 2022 by the Society for Vascular Surgery. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wolf, Katharine G.
Crawford, Emily B.
Wartan, Nora M.
Schneiderman, Sylvia K.
Riehl, Valerie E.
Dambaeva, Svetlana V.
Beaman, Kenneth D.
Ephrin-B2-expressing natural killer cells induce angiogenesis
title Ephrin-B2-expressing natural killer cells induce angiogenesis
title_full Ephrin-B2-expressing natural killer cells induce angiogenesis
title_fullStr Ephrin-B2-expressing natural killer cells induce angiogenesis
title_full_unstemmed Ephrin-B2-expressing natural killer cells induce angiogenesis
title_short Ephrin-B2-expressing natural killer cells induce angiogenesis
title_sort ephrin-b2-expressing natural killer cells induce angiogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692026/
https://www.ncbi.nlm.nih.gov/pubmed/36439700
http://dx.doi.org/10.1016/j.jvssci.2022.08.003
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