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Ephrin-B2-expressing natural killer cells induce angiogenesis
BACKGROUND: Therapeutic angiogenesis aims to induce new blood vessel growth in ischemic tissues; however, previous clinical trials have had limited success. Studies of uterine angiogenesis revealed a specialized subset of natural killer (NK) cells, called uterine NK (uNK) cells, which have unique pr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692026/ https://www.ncbi.nlm.nih.gov/pubmed/36439700 http://dx.doi.org/10.1016/j.jvssci.2022.08.003 |
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author | Wolf, Katharine G. Crawford, Emily B. Wartan, Nora M. Schneiderman, Sylvia K. Riehl, Valerie E. Dambaeva, Svetlana V. Beaman, Kenneth D. |
author_facet | Wolf, Katharine G. Crawford, Emily B. Wartan, Nora M. Schneiderman, Sylvia K. Riehl, Valerie E. Dambaeva, Svetlana V. Beaman, Kenneth D. |
author_sort | Wolf, Katharine G. |
collection | PubMed |
description | BACKGROUND: Therapeutic angiogenesis aims to induce new blood vessel growth in ischemic tissues; however, previous clinical trials have had limited success. Studies of uterine angiogenesis revealed a specialized subset of natural killer (NK) cells, called uterine NK (uNK) cells, which have unique proangiogenic abilities. METHODS: We show that uNK cells in mice express ephrin-B2, a regulator of angiogenesis, to induce tubule formation in an ex vivo coculture tubule formation assay. We next induced the expression of ephrin-B2 by splenic NK (sNK) cells harvested from male mice. RESULTS: We showed that induced NK (iNK) cells can also instruct endothelial cells to form tubules using ephrin-B2. CONCLUSIONS: We concluded that Ephrin-B2 is a marker of proangiogenic uNK cells and that a proangiogenic phenotype characterized by ephrin-B2 can be induced in sNK cells to induce therapeutic angiogenesis. |
format | Online Article Text |
id | pubmed-9692026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96920262022-11-26 Ephrin-B2-expressing natural killer cells induce angiogenesis Wolf, Katharine G. Crawford, Emily B. Wartan, Nora M. Schneiderman, Sylvia K. Riehl, Valerie E. Dambaeva, Svetlana V. Beaman, Kenneth D. JVS Vasc Sci Article BACKGROUND: Therapeutic angiogenesis aims to induce new blood vessel growth in ischemic tissues; however, previous clinical trials have had limited success. Studies of uterine angiogenesis revealed a specialized subset of natural killer (NK) cells, called uterine NK (uNK) cells, which have unique proangiogenic abilities. METHODS: We show that uNK cells in mice express ephrin-B2, a regulator of angiogenesis, to induce tubule formation in an ex vivo coculture tubule formation assay. We next induced the expression of ephrin-B2 by splenic NK (sNK) cells harvested from male mice. RESULTS: We showed that induced NK (iNK) cells can also instruct endothelial cells to form tubules using ephrin-B2. CONCLUSIONS: We concluded that Ephrin-B2 is a marker of proangiogenic uNK cells and that a proangiogenic phenotype characterized by ephrin-B2 can be induced in sNK cells to induce therapeutic angiogenesis. Elsevier 2022-10-27 /pmc/articles/PMC9692026/ /pubmed/36439700 http://dx.doi.org/10.1016/j.jvssci.2022.08.003 Text en Copyright © 2022 by the Society for Vascular Surgery. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wolf, Katharine G. Crawford, Emily B. Wartan, Nora M. Schneiderman, Sylvia K. Riehl, Valerie E. Dambaeva, Svetlana V. Beaman, Kenneth D. Ephrin-B2-expressing natural killer cells induce angiogenesis |
title | Ephrin-B2-expressing natural killer cells induce angiogenesis |
title_full | Ephrin-B2-expressing natural killer cells induce angiogenesis |
title_fullStr | Ephrin-B2-expressing natural killer cells induce angiogenesis |
title_full_unstemmed | Ephrin-B2-expressing natural killer cells induce angiogenesis |
title_short | Ephrin-B2-expressing natural killer cells induce angiogenesis |
title_sort | ephrin-b2-expressing natural killer cells induce angiogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692026/ https://www.ncbi.nlm.nih.gov/pubmed/36439700 http://dx.doi.org/10.1016/j.jvssci.2022.08.003 |
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