Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells

Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs)....

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Detalles Bibliográficos
Autores principales: Ayaz-Guner, Serife, Acar, Mustafa Burak, Boyvat, Dudu, Guner, Huseyin, Bozalan, Habibe, Güzel, Melis, Yıldır, Selin Kübra, Altınsoy, Nilay, Fındık, Fatma, Karakükçü, Musa, Özcan, Servet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692030/
https://www.ncbi.nlm.nih.gov/pubmed/36595912
http://dx.doi.org/10.1016/j.xpro.2022.101863

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692030/
https://www.ncbi.nlm.nih.gov/pubmed/36595912
http://dx.doi.org/10.1016/j.xpro.2022.101863

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