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Is There a Universal Endurance Microbiota?

Billions of microbes sculpt the gut ecosystem, affecting physiology. Since endurance athletes’ performance is often physiology-limited, understanding the composition and interactions within athletes’ gut microbiota could improve performance. Individual studies describe differences in the relative ab...

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Detalles Bibliográficos
Autores principales: Olbricht, Hope, Twadell, Kaitlyn, Sandel, Brody, Stephens, Craig, Whittall, Justen B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692344/
https://www.ncbi.nlm.nih.gov/pubmed/36363806
http://dx.doi.org/10.3390/microorganisms10112213
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author Olbricht, Hope
Twadell, Kaitlyn
Sandel, Brody
Stephens, Craig
Whittall, Justen B.
author_facet Olbricht, Hope
Twadell, Kaitlyn
Sandel, Brody
Stephens, Craig
Whittall, Justen B.
author_sort Olbricht, Hope
collection PubMed
description Billions of microbes sculpt the gut ecosystem, affecting physiology. Since endurance athletes’ performance is often physiology-limited, understanding the composition and interactions within athletes’ gut microbiota could improve performance. Individual studies describe differences in the relative abundance of bacterial taxa in endurance athletes, suggesting the existence of an “endurance microbiota”, yet the taxa identified are mostly non-overlapping. To narrow down the source of this variation, we created a bioinformatics workflow and reanalyzed fecal microbiota from four 16S rRNA gene sequence datasets associated with endurance athletes and controls, examining diversity, relative abundance, correlations, and association networks. There were no significant differences in alpha diversity among all datasets and only one out of four datasets showed a significant overall difference in bacterial community abundance. When bacteria were examined individually, there were no genera with significantly different relative abundance in all four datasets. Two genera were significantly different in two datasets (Veillonella and Romboutsia). No changes in correlated abundances were consistent across datasets. A power analysis using the variance in relative abundance detected in each dataset indicated that much larger sample sizes will be necessary to detect a modest difference in relative abundance especially given the multitude of covariates. Our analysis confirms several challenges when comparing microbiota in general, and indicates that microbes consistently or universally associated with human endurance remain elusive.
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spelling pubmed-96923442022-11-26 Is There a Universal Endurance Microbiota? Olbricht, Hope Twadell, Kaitlyn Sandel, Brody Stephens, Craig Whittall, Justen B. Microorganisms Article Billions of microbes sculpt the gut ecosystem, affecting physiology. Since endurance athletes’ performance is often physiology-limited, understanding the composition and interactions within athletes’ gut microbiota could improve performance. Individual studies describe differences in the relative abundance of bacterial taxa in endurance athletes, suggesting the existence of an “endurance microbiota”, yet the taxa identified are mostly non-overlapping. To narrow down the source of this variation, we created a bioinformatics workflow and reanalyzed fecal microbiota from four 16S rRNA gene sequence datasets associated with endurance athletes and controls, examining diversity, relative abundance, correlations, and association networks. There were no significant differences in alpha diversity among all datasets and only one out of four datasets showed a significant overall difference in bacterial community abundance. When bacteria were examined individually, there were no genera with significantly different relative abundance in all four datasets. Two genera were significantly different in two datasets (Veillonella and Romboutsia). No changes in correlated abundances were consistent across datasets. A power analysis using the variance in relative abundance detected in each dataset indicated that much larger sample sizes will be necessary to detect a modest difference in relative abundance especially given the multitude of covariates. Our analysis confirms several challenges when comparing microbiota in general, and indicates that microbes consistently or universally associated with human endurance remain elusive. MDPI 2022-11-09 /pmc/articles/PMC9692344/ /pubmed/36363806 http://dx.doi.org/10.3390/microorganisms10112213 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olbricht, Hope
Twadell, Kaitlyn
Sandel, Brody
Stephens, Craig
Whittall, Justen B.
Is There a Universal Endurance Microbiota?
title Is There a Universal Endurance Microbiota?
title_full Is There a Universal Endurance Microbiota?
title_fullStr Is There a Universal Endurance Microbiota?
title_full_unstemmed Is There a Universal Endurance Microbiota?
title_short Is There a Universal Endurance Microbiota?
title_sort is there a universal endurance microbiota?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692344/
https://www.ncbi.nlm.nih.gov/pubmed/36363806
http://dx.doi.org/10.3390/microorganisms10112213
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