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Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies

Arthrospira platensis (commercially known as Spirulina) is an excellent source of phycobiliproteins, especially C-phycocyanin. Phycobiliproteins are significant bioactive compounds with useful biological applications. The extraction process plays a significant role in downstream microalga production...

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Autores principales: Pan-utai, Wanida, Iamtham, Siriluck, Boonbumrung, Sumitra, Mookdasanit, Juta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692409/
https://www.ncbi.nlm.nih.gov/pubmed/36431030
http://dx.doi.org/10.3390/life12111896
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author Pan-utai, Wanida
Iamtham, Siriluck
Boonbumrung, Sumitra
Mookdasanit, Juta
author_facet Pan-utai, Wanida
Iamtham, Siriluck
Boonbumrung, Sumitra
Mookdasanit, Juta
author_sort Pan-utai, Wanida
collection PubMed
description Arthrospira platensis (commercially known as Spirulina) is an excellent source of phycobiliproteins, especially C-phycocyanin. Phycobiliproteins are significant bioactive compounds with useful biological applications. The extraction process plays a significant role in downstream microalga production and utilisation. The important pigments found in A. platensis include chlorophyll and carotenoids as nonpolar pigments and phycobiliproteins as polar pigments. Supercritical fluid extraction (SFE) as a green extraction technology for the high-value metabolites of microalgae has potential for trends in food and human health. The nonpolar bioactive compounds, chlorophyll and carotenoids of A. platensis, were primarily separated using supercritical carbon dioxide (SC-CO(2)) solvent-free fluid extraction pressure; the temperature and ethanol as cosolvent conditions were compared. The residue from the A. platensis cells was subjected to phycobiliprotein extraction. The phosphate and water extraction of A. platensis SFE residue were compared to evaluate phycobiliprotein extraction. The SFE results exhibited higher pressure (350 bar) and temperature extraction (50 °C) with ethanol-free extraction and increased nonpolar pigment. Phycobiliprotein yield was obtained from A. platensis SFE residue by ethanol-free buffer extraction as a suitable process with antioxidant properties. The C-phycocyanin was isolated and enhanced to 0.7 purity as food grade. This developed method can be used as a guideline and applied as a sustainable process for important pigment extraction from Arthrospira microalgae.
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spelling pubmed-96924092022-11-26 Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies Pan-utai, Wanida Iamtham, Siriluck Boonbumrung, Sumitra Mookdasanit, Juta Life (Basel) Article Arthrospira platensis (commercially known as Spirulina) is an excellent source of phycobiliproteins, especially C-phycocyanin. Phycobiliproteins are significant bioactive compounds with useful biological applications. The extraction process plays a significant role in downstream microalga production and utilisation. The important pigments found in A. platensis include chlorophyll and carotenoids as nonpolar pigments and phycobiliproteins as polar pigments. Supercritical fluid extraction (SFE) as a green extraction technology for the high-value metabolites of microalgae has potential for trends in food and human health. The nonpolar bioactive compounds, chlorophyll and carotenoids of A. platensis, were primarily separated using supercritical carbon dioxide (SC-CO(2)) solvent-free fluid extraction pressure; the temperature and ethanol as cosolvent conditions were compared. The residue from the A. platensis cells was subjected to phycobiliprotein extraction. The phosphate and water extraction of A. platensis SFE residue were compared to evaluate phycobiliprotein extraction. The SFE results exhibited higher pressure (350 bar) and temperature extraction (50 °C) with ethanol-free extraction and increased nonpolar pigment. Phycobiliprotein yield was obtained from A. platensis SFE residue by ethanol-free buffer extraction as a suitable process with antioxidant properties. The C-phycocyanin was isolated and enhanced to 0.7 purity as food grade. This developed method can be used as a guideline and applied as a sustainable process for important pigment extraction from Arthrospira microalgae. MDPI 2022-11-15 /pmc/articles/PMC9692409/ /pubmed/36431030 http://dx.doi.org/10.3390/life12111896 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pan-utai, Wanida
Iamtham, Siriluck
Boonbumrung, Sumitra
Mookdasanit, Juta
Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies
title Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies
title_full Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies
title_fullStr Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies
title_full_unstemmed Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies
title_short Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies
title_sort improvement in the sequential extraction of phycobiliproteins from arthrospira platensis using green technologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692409/
https://www.ncbi.nlm.nih.gov/pubmed/36431030
http://dx.doi.org/10.3390/life12111896
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