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Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation

Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties o...

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Autores principales: Islam, Shirmin, Mahmud, Md. Liton, Almalki, Waleed H., Biswas, Suvro, Islam, Md. Ariful, Mortuza, Md. Golam, Hossain, Mohammad Akbar, Ekram, Md. Akhtar-E, Uddin, Md. Salah, Zaman, Shahriar, Saleh, Md. Abu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692604/
https://www.ncbi.nlm.nih.gov/pubmed/36363697
http://dx.doi.org/10.3390/microorganisms10112105
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author Islam, Shirmin
Mahmud, Md. Liton
Almalki, Waleed H.
Biswas, Suvro
Islam, Md. Ariful
Mortuza, Md. Golam
Hossain, Mohammad Akbar
Ekram, Md. Akhtar-E
Uddin, Md. Salah
Zaman, Shahriar
Saleh, Md. Abu
author_facet Islam, Shirmin
Mahmud, Md. Liton
Almalki, Waleed H.
Biswas, Suvro
Islam, Md. Ariful
Mortuza, Md. Golam
Hossain, Mohammad Akbar
Ekram, Md. Akhtar-E
Uddin, Md. Salah
Zaman, Shahriar
Saleh, Md. Abu
author_sort Islam, Shirmin
collection PubMed
description Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties of bacterial bioactive compounds has been sparse. In this study, the efficacy of compounds from a cell-free supernatant of Bacillus subtilis against a biofilm formation of Pseudomonas sp. was studied through in vitro, in vivo and in silico studies. Here, in well diffusion method, Bacillus subtilis demonstrated antibacterial activity, and more than 50% biofilm inhibition activity against Pseudomonas sp. was exhibited through in vitro studies. Moreover, molecular docking and molecular dynamics (MD) simulation gave insights into the possible mode of action of the bacterial volatile compounds identified through GC-MS to inhibit the biofilm-formation protein (PDB ID: 7M1M) of Pseudomonas sp. The binding energy revealed from docking studies ranged from −2.3 to −7.0 kcal mol(−1). Moreover, 1-(9H-Fluoren-2-yl)-2-(1-phenyl-1H-ttetrazole5-ylsulfanyl)-ethanone was found to be the best-docked compound through ADMET and pharmacokinetic properties. Furthermore, MD simulations further supported the in vitro studies and formed a stable complex with the tested protein. Thus, this study gives an insight into the development of new antibiotics to combat multi-drug-resistant bacteria.
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spelling pubmed-96926042022-11-26 Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation Islam, Shirmin Mahmud, Md. Liton Almalki, Waleed H. Biswas, Suvro Islam, Md. Ariful Mortuza, Md. Golam Hossain, Mohammad Akbar Ekram, Md. Akhtar-E Uddin, Md. Salah Zaman, Shahriar Saleh, Md. Abu Microorganisms Article Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties of bacterial bioactive compounds has been sparse. In this study, the efficacy of compounds from a cell-free supernatant of Bacillus subtilis against a biofilm formation of Pseudomonas sp. was studied through in vitro, in vivo and in silico studies. Here, in well diffusion method, Bacillus subtilis demonstrated antibacterial activity, and more than 50% biofilm inhibition activity against Pseudomonas sp. was exhibited through in vitro studies. Moreover, molecular docking and molecular dynamics (MD) simulation gave insights into the possible mode of action of the bacterial volatile compounds identified through GC-MS to inhibit the biofilm-formation protein (PDB ID: 7M1M) of Pseudomonas sp. The binding energy revealed from docking studies ranged from −2.3 to −7.0 kcal mol(−1). Moreover, 1-(9H-Fluoren-2-yl)-2-(1-phenyl-1H-ttetrazole5-ylsulfanyl)-ethanone was found to be the best-docked compound through ADMET and pharmacokinetic properties. Furthermore, MD simulations further supported the in vitro studies and formed a stable complex with the tested protein. Thus, this study gives an insight into the development of new antibiotics to combat multi-drug-resistant bacteria. MDPI 2022-10-24 /pmc/articles/PMC9692604/ /pubmed/36363697 http://dx.doi.org/10.3390/microorganisms10112105 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Islam, Shirmin
Mahmud, Md. Liton
Almalki, Waleed H.
Biswas, Suvro
Islam, Md. Ariful
Mortuza, Md. Golam
Hossain, Mohammad Akbar
Ekram, Md. Akhtar-E
Uddin, Md. Salah
Zaman, Shahriar
Saleh, Md. Abu
Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
title Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
title_full Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
title_fullStr Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
title_full_unstemmed Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
title_short Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
title_sort cell-free supernatants (cfss) from the culture of bacillus subtilis inhibit pseudomonas sp. biofilm formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692604/
https://www.ncbi.nlm.nih.gov/pubmed/36363697
http://dx.doi.org/10.3390/microorganisms10112105
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