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Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation
Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692604/ https://www.ncbi.nlm.nih.gov/pubmed/36363697 http://dx.doi.org/10.3390/microorganisms10112105 |
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author | Islam, Shirmin Mahmud, Md. Liton Almalki, Waleed H. Biswas, Suvro Islam, Md. Ariful Mortuza, Md. Golam Hossain, Mohammad Akbar Ekram, Md. Akhtar-E Uddin, Md. Salah Zaman, Shahriar Saleh, Md. Abu |
author_facet | Islam, Shirmin Mahmud, Md. Liton Almalki, Waleed H. Biswas, Suvro Islam, Md. Ariful Mortuza, Md. Golam Hossain, Mohammad Akbar Ekram, Md. Akhtar-E Uddin, Md. Salah Zaman, Shahriar Saleh, Md. Abu |
author_sort | Islam, Shirmin |
collection | PubMed |
description | Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties of bacterial bioactive compounds has been sparse. In this study, the efficacy of compounds from a cell-free supernatant of Bacillus subtilis against a biofilm formation of Pseudomonas sp. was studied through in vitro, in vivo and in silico studies. Here, in well diffusion method, Bacillus subtilis demonstrated antibacterial activity, and more than 50% biofilm inhibition activity against Pseudomonas sp. was exhibited through in vitro studies. Moreover, molecular docking and molecular dynamics (MD) simulation gave insights into the possible mode of action of the bacterial volatile compounds identified through GC-MS to inhibit the biofilm-formation protein (PDB ID: 7M1M) of Pseudomonas sp. The binding energy revealed from docking studies ranged from −2.3 to −7.0 kcal mol(−1). Moreover, 1-(9H-Fluoren-2-yl)-2-(1-phenyl-1H-ttetrazole5-ylsulfanyl)-ethanone was found to be the best-docked compound through ADMET and pharmacokinetic properties. Furthermore, MD simulations further supported the in vitro studies and formed a stable complex with the tested protein. Thus, this study gives an insight into the development of new antibiotics to combat multi-drug-resistant bacteria. |
format | Online Article Text |
id | pubmed-9692604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96926042022-11-26 Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation Islam, Shirmin Mahmud, Md. Liton Almalki, Waleed H. Biswas, Suvro Islam, Md. Ariful Mortuza, Md. Golam Hossain, Mohammad Akbar Ekram, Md. Akhtar-E Uddin, Md. Salah Zaman, Shahriar Saleh, Md. Abu Microorganisms Article Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties of bacterial bioactive compounds has been sparse. In this study, the efficacy of compounds from a cell-free supernatant of Bacillus subtilis against a biofilm formation of Pseudomonas sp. was studied through in vitro, in vivo and in silico studies. Here, in well diffusion method, Bacillus subtilis demonstrated antibacterial activity, and more than 50% biofilm inhibition activity against Pseudomonas sp. was exhibited through in vitro studies. Moreover, molecular docking and molecular dynamics (MD) simulation gave insights into the possible mode of action of the bacterial volatile compounds identified through GC-MS to inhibit the biofilm-formation protein (PDB ID: 7M1M) of Pseudomonas sp. The binding energy revealed from docking studies ranged from −2.3 to −7.0 kcal mol(−1). Moreover, 1-(9H-Fluoren-2-yl)-2-(1-phenyl-1H-ttetrazole5-ylsulfanyl)-ethanone was found to be the best-docked compound through ADMET and pharmacokinetic properties. Furthermore, MD simulations further supported the in vitro studies and formed a stable complex with the tested protein. Thus, this study gives an insight into the development of new antibiotics to combat multi-drug-resistant bacteria. MDPI 2022-10-24 /pmc/articles/PMC9692604/ /pubmed/36363697 http://dx.doi.org/10.3390/microorganisms10112105 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Islam, Shirmin Mahmud, Md. Liton Almalki, Waleed H. Biswas, Suvro Islam, Md. Ariful Mortuza, Md. Golam Hossain, Mohammad Akbar Ekram, Md. Akhtar-E Uddin, Md. Salah Zaman, Shahriar Saleh, Md. Abu Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation |
title | Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation |
title_full | Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation |
title_fullStr | Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation |
title_full_unstemmed | Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation |
title_short | Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation |
title_sort | cell-free supernatants (cfss) from the culture of bacillus subtilis inhibit pseudomonas sp. biofilm formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692604/ https://www.ncbi.nlm.nih.gov/pubmed/36363697 http://dx.doi.org/10.3390/microorganisms10112105 |
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