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Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes

The massive accumulation of plastics over the decades in the aquatic environment has led to the dispersion of plastic components in aquatic ecosystems, invading the food webs. Plastics fragmented into microplastics can be bioaccumulated by fishes via different exposure routes, causing several advers...

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Autores principales: Bobori, Dimitra C., Feidantsis, Konstantinos, Dimitriadi, Anastasia, Datsi, Nefeli, Ripis, Panagiotis, Kalogiannis, Stavros, Sampsonidis, Ioannis, Kastrinaki, Georgia, Ainali, Nina Maria, Lambropoulou, Dimitra A., Kyzas, George Z., Koumoundouros, George, Bikiaris, Dimitrios N., Kaloyianni, Martha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692651/
https://www.ncbi.nlm.nih.gov/pubmed/36430357
http://dx.doi.org/10.3390/ijms232213878
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author Bobori, Dimitra C.
Feidantsis, Konstantinos
Dimitriadi, Anastasia
Datsi, Nefeli
Ripis, Panagiotis
Kalogiannis, Stavros
Sampsonidis, Ioannis
Kastrinaki, Georgia
Ainali, Nina Maria
Lambropoulou, Dimitra A.
Kyzas, George Z.
Koumoundouros, George
Bikiaris, Dimitrios N.
Kaloyianni, Martha
author_facet Bobori, Dimitra C.
Feidantsis, Konstantinos
Dimitriadi, Anastasia
Datsi, Nefeli
Ripis, Panagiotis
Kalogiannis, Stavros
Sampsonidis, Ioannis
Kastrinaki, Georgia
Ainali, Nina Maria
Lambropoulou, Dimitra A.
Kyzas, George Z.
Koumoundouros, George
Bikiaris, Dimitrios N.
Kaloyianni, Martha
author_sort Bobori, Dimitra C.
collection PubMed
description The massive accumulation of plastics over the decades in the aquatic environment has led to the dispersion of plastic components in aquatic ecosystems, invading the food webs. Plastics fragmented into microplastics can be bioaccumulated by fishes via different exposure routes, causing several adverse effects. In the present study, the dose-dependent cytotoxicity of 8–10 μm polypropylene microplastics (PP-MPs), at concentrations of 1 mg/g (low dose) and 10 mg/g dry food (high dose), was evaluated in the liver and gill tissues of two fish species, the zebrafish (Danio rerio) and the freshwater perch (Perca fluviatilis). According to our results, the inclusion of PP-MPs in the feed of D. rerio and P. fluviatilis hampered the cellular function of the gills and hepatic cells by lipid peroxidation, DNA damage, protein ubiquitination, apoptosis, autophagy, and changes in metabolite concentration, providing evidence that the toxicity of PP-MPs is dose dependent. With regard to the individual assays tested in the present study, the biggest impact was observed in DNA damage, which exhibited a maximum increase of 18.34-fold in the liver of D. rerio. The sensitivity of the two fish species studied differed, while no clear tissue specificity in both fish species was observed. The metabolome of both tissues was altered in both treatments, while tryptophan and nicotinic acid exhibited the greatest decrease among all metabolites in all treatments in comparison to the control. The battery of biomarkers used in the present study as well as metabolomic changes could be suggested as early-warning signals for the assessment of the aquatic environment quality against MPs. In addition, our results contribute to the elucidation of the mechanism induced by nanomaterials on tissues of aquatic organisms, since comprehending the magnitude of their impact on aquatic ecosystems is of great importance.
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spelling pubmed-96926512022-11-26 Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes Bobori, Dimitra C. Feidantsis, Konstantinos Dimitriadi, Anastasia Datsi, Nefeli Ripis, Panagiotis Kalogiannis, Stavros Sampsonidis, Ioannis Kastrinaki, Georgia Ainali, Nina Maria Lambropoulou, Dimitra A. Kyzas, George Z. Koumoundouros, George Bikiaris, Dimitrios N. Kaloyianni, Martha Int J Mol Sci Article The massive accumulation of plastics over the decades in the aquatic environment has led to the dispersion of plastic components in aquatic ecosystems, invading the food webs. Plastics fragmented into microplastics can be bioaccumulated by fishes via different exposure routes, causing several adverse effects. In the present study, the dose-dependent cytotoxicity of 8–10 μm polypropylene microplastics (PP-MPs), at concentrations of 1 mg/g (low dose) and 10 mg/g dry food (high dose), was evaluated in the liver and gill tissues of two fish species, the zebrafish (Danio rerio) and the freshwater perch (Perca fluviatilis). According to our results, the inclusion of PP-MPs in the feed of D. rerio and P. fluviatilis hampered the cellular function of the gills and hepatic cells by lipid peroxidation, DNA damage, protein ubiquitination, apoptosis, autophagy, and changes in metabolite concentration, providing evidence that the toxicity of PP-MPs is dose dependent. With regard to the individual assays tested in the present study, the biggest impact was observed in DNA damage, which exhibited a maximum increase of 18.34-fold in the liver of D. rerio. The sensitivity of the two fish species studied differed, while no clear tissue specificity in both fish species was observed. The metabolome of both tissues was altered in both treatments, while tryptophan and nicotinic acid exhibited the greatest decrease among all metabolites in all treatments in comparison to the control. The battery of biomarkers used in the present study as well as metabolomic changes could be suggested as early-warning signals for the assessment of the aquatic environment quality against MPs. In addition, our results contribute to the elucidation of the mechanism induced by nanomaterials on tissues of aquatic organisms, since comprehending the magnitude of their impact on aquatic ecosystems is of great importance. MDPI 2022-11-10 /pmc/articles/PMC9692651/ /pubmed/36430357 http://dx.doi.org/10.3390/ijms232213878 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bobori, Dimitra C.
Feidantsis, Konstantinos
Dimitriadi, Anastasia
Datsi, Nefeli
Ripis, Panagiotis
Kalogiannis, Stavros
Sampsonidis, Ioannis
Kastrinaki, Georgia
Ainali, Nina Maria
Lambropoulou, Dimitra A.
Kyzas, George Z.
Koumoundouros, George
Bikiaris, Dimitrios N.
Kaloyianni, Martha
Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
title Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
title_full Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
title_fullStr Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
title_full_unstemmed Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
title_short Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
title_sort dose-dependent cytotoxicity of polypropylene microplastics (pp-mps) in two freshwater fishes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692651/
https://www.ncbi.nlm.nih.gov/pubmed/36430357
http://dx.doi.org/10.3390/ijms232213878
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