Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody

Echovirus 3 (E3), a serotype of human enterovirus B (HEV-B), causes severe diseases in infants. Here, we determined the structures of E3 with a monoclonal antibody (MAb) 6D10 by cryo-EM to comprehensively understand the specificities and the immunological characteristic of this serotype. The solved...

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Autores principales: Qi, Shuai, Fu, Wangjun, Fan, Jinyan, Zhang, Li, Zheng, Binyang, Wang, Kang, Wang, Xiangxi, Zhu, Ling, Li, Xinjian, Zhang, Yuxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692665/
https://www.ncbi.nlm.nih.gov/pubmed/36366420
http://dx.doi.org/10.3390/v14112322
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author Qi, Shuai
Fu, Wangjun
Fan, Jinyan
Zhang, Li
Zheng, Binyang
Wang, Kang
Wang, Xiangxi
Zhu, Ling
Li, Xinjian
Zhang, Yuxia
author_facet Qi, Shuai
Fu, Wangjun
Fan, Jinyan
Zhang, Li
Zheng, Binyang
Wang, Kang
Wang, Xiangxi
Zhu, Ling
Li, Xinjian
Zhang, Yuxia
author_sort Qi, Shuai
collection PubMed
description Echovirus 3 (E3), a serotype of human enterovirus B (HEV-B), causes severe diseases in infants. Here, we determined the structures of E3 with a monoclonal antibody (MAb) 6D10 by cryo-EM to comprehensively understand the specificities and the immunological characteristic of this serotype. The solved cryo-EM structures of the F-, A-, and E-particles of E3 bound with 6D10 revealed the structural features of the virus–antibody interface. Importantly, the structures of E-particles bound with 6D10 revealed for the first time the nature of the C-terminus of VP1 for HEV-Bs at the structural level. The highly immunogenic nature of this region in the E-particles provides new strategies for vaccine development for HEV-Bs.
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spelling pubmed-96926652022-11-26 Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody Qi, Shuai Fu, Wangjun Fan, Jinyan Zhang, Li Zheng, Binyang Wang, Kang Wang, Xiangxi Zhu, Ling Li, Xinjian Zhang, Yuxia Viruses Brief Report Echovirus 3 (E3), a serotype of human enterovirus B (HEV-B), causes severe diseases in infants. Here, we determined the structures of E3 with a monoclonal antibody (MAb) 6D10 by cryo-EM to comprehensively understand the specificities and the immunological characteristic of this serotype. The solved cryo-EM structures of the F-, A-, and E-particles of E3 bound with 6D10 revealed the structural features of the virus–antibody interface. Importantly, the structures of E-particles bound with 6D10 revealed for the first time the nature of the C-terminus of VP1 for HEV-Bs at the structural level. The highly immunogenic nature of this region in the E-particles provides new strategies for vaccine development for HEV-Bs. MDPI 2022-10-22 /pmc/articles/PMC9692665/ /pubmed/36366420 http://dx.doi.org/10.3390/v14112322 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Qi, Shuai
Fu, Wangjun
Fan, Jinyan
Zhang, Li
Zheng, Binyang
Wang, Kang
Wang, Xiangxi
Zhu, Ling
Li, Xinjian
Zhang, Yuxia
Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody
title Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody
title_full Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody
title_fullStr Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody
title_full_unstemmed Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody
title_short Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody
title_sort structural basis for the immunogenicity of the c-terminus of vp1 of echovirus 3 revealed by the binding of a neutralizing antibody
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692665/
https://www.ncbi.nlm.nih.gov/pubmed/36366420
http://dx.doi.org/10.3390/v14112322
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