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Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis

Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling T. pyogenes infections in the animal husbandry industry. Luteolin is a naturally derived flavonoid comp...

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Autores principales: Zhang, Luyao, Cai, Yitong, Li, Lishuang, Chen, Chen, Zhao, Hanyu, Zhang, Zehui, Liu, Yaochuan, Wang, Yingyu, Tian, Chunlian, Liu, Mingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692790/
https://www.ncbi.nlm.nih.gov/pubmed/36430929
http://dx.doi.org/10.3390/ijms232214451
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author Zhang, Luyao
Cai, Yitong
Li, Lishuang
Chen, Chen
Zhao, Hanyu
Zhang, Zehui
Liu, Yaochuan
Wang, Yingyu
Tian, Chunlian
Liu, Mingchun
author_facet Zhang, Luyao
Cai, Yitong
Li, Lishuang
Chen, Chen
Zhao, Hanyu
Zhang, Zehui
Liu, Yaochuan
Wang, Yingyu
Tian, Chunlian
Liu, Mingchun
author_sort Zhang, Luyao
collection PubMed
description Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling T. pyogenes infections in the animal husbandry industry. Luteolin is a naturally derived flavonoid compound with antibacterial properties. In this study, the effects and the mechanism of luteolin on T. pyogenes biofilm were analyzed and explored. The MBIC and MBEC of luteolin on T. pyogenes were 156 μg/mL and 312 μg/mL, respectively. The anti-biofilm effects of luteolin were also observed by a confocal laser microscope and scanning electron microscope. The results indicated that 312 μg/mL of luteolin could disperse large pieces of biofilm into small clusters after 8 h of treatment. According to the real-time quantitative PCR detection results, luteolin could significantly inhibit the relative expression of the biofilm-associated genes luxS, plo, rbsB and lsrB. In addition, the in vivo anti-biofilm activity of luteolin against T. pyogenes was studied using a rat endometritis model established by glacial acetic acid stimulation and T. pyogenes intrauterine infusion. Our study showed that luteolin could significantly reduce the symptoms of rat endometritis. These data may provide new opinions on the clinical treatment of luteolin and other flavonoid compounds on T. pyogenes biofilm-associated infections.
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spelling pubmed-96927902022-11-26 Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis Zhang, Luyao Cai, Yitong Li, Lishuang Chen, Chen Zhao, Hanyu Zhang, Zehui Liu, Yaochuan Wang, Yingyu Tian, Chunlian Liu, Mingchun Int J Mol Sci Article Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling T. pyogenes infections in the animal husbandry industry. Luteolin is a naturally derived flavonoid compound with antibacterial properties. In this study, the effects and the mechanism of luteolin on T. pyogenes biofilm were analyzed and explored. The MBIC and MBEC of luteolin on T. pyogenes were 156 μg/mL and 312 μg/mL, respectively. The anti-biofilm effects of luteolin were also observed by a confocal laser microscope and scanning electron microscope. The results indicated that 312 μg/mL of luteolin could disperse large pieces of biofilm into small clusters after 8 h of treatment. According to the real-time quantitative PCR detection results, luteolin could significantly inhibit the relative expression of the biofilm-associated genes luxS, plo, rbsB and lsrB. In addition, the in vivo anti-biofilm activity of luteolin against T. pyogenes was studied using a rat endometritis model established by glacial acetic acid stimulation and T. pyogenes intrauterine infusion. Our study showed that luteolin could significantly reduce the symptoms of rat endometritis. These data may provide new opinions on the clinical treatment of luteolin and other flavonoid compounds on T. pyogenes biofilm-associated infections. MDPI 2022-11-21 /pmc/articles/PMC9692790/ /pubmed/36430929 http://dx.doi.org/10.3390/ijms232214451 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Luyao
Cai, Yitong
Li, Lishuang
Chen, Chen
Zhao, Hanyu
Zhang, Zehui
Liu, Yaochuan
Wang, Yingyu
Tian, Chunlian
Liu, Mingchun
Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis
title Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis
title_full Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis
title_fullStr Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis
title_full_unstemmed Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis
title_short Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis
title_sort effects of luteolin on biofilm of trueperella pyogenes and its therapeutic effect on rat endometritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692790/
https://www.ncbi.nlm.nih.gov/pubmed/36430929
http://dx.doi.org/10.3390/ijms232214451
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