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Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes
Fascioloidosis is a parasitic disease caused by a trematode Fascioloides magna. Since major histocompatibility complex (MHC) genes play an important role in the immune response, the aim of this study was to compare the potential differences in MHC class II SLA-DRB1 exon 2 genes between wild boar pop...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692795/ https://www.ncbi.nlm.nih.gov/pubmed/36422610 http://dx.doi.org/10.3390/pathogens11111359 |
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author | Konjević, Dean Erman, Vlatka Bujanić, Miljenko Svetličić, Ida Arbanasić, Haidi Lubura Strunjak, Snježana Galov, Ana |
author_facet | Konjević, Dean Erman, Vlatka Bujanić, Miljenko Svetličić, Ida Arbanasić, Haidi Lubura Strunjak, Snježana Galov, Ana |
author_sort | Konjević, Dean |
collection | PubMed |
description | Fascioloidosis is a parasitic disease caused by a trematode Fascioloides magna. Since major histocompatibility complex (MHC) genes play an important role in the immune response, the aim of this study was to compare the potential differences in MHC class II SLA-DRB1 exon 2 genes between wild boar populations from infected (cases) and non-infected areas (controls). During the winter of 2021, a total of 136 wild boar tissue samples were collected, 39 cases and 97 controls. DNA was extracted and sequenced using the Illumina platform. Differences in distributions of allele combinations were calculated using the Chi-Square test for homogeneity and between proportions using the large-sample test and Fisher–Irwin test. Analysis revealed 19 previously described swine leucocyte antigen (SLA) alleles. The number of polymorphic sites was 79 (29.6%), with 99 mutations in total. Nucleotide diversity π was estimated at 0.11. Proportions of the alleles SLA-DRB1*12:05 (p = 0.0008379) and SLA-DRB1*0101 (p = 0.0002825) were statistically significantly higher in controls, and proportions of the SLA-DRB1*0602 (p = 0.006059) and SLA-DRB1*0901 (p = 0.0006601) in cases. Alleles SLA-DRB1*04:09, SLA-DRB1*0501, SLA-DRB1*11:09, and SLA-DRB1*1301 were detected only in cases, while SLA-DRB1*0404, SLA-DRB1*0701, SLA-DRB1*02:10, and SLA-DRB1*04:08 were present only in controls. We did not confirm the existence of specific alleles that could be linked to F. magna infection. Detected high variability of the MHC class II SLA-DRB1 exon 2 genes indicate high resistance potential against various pathogens. |
format | Online Article Text |
id | pubmed-9692795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96927952022-11-26 Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes Konjević, Dean Erman, Vlatka Bujanić, Miljenko Svetličić, Ida Arbanasić, Haidi Lubura Strunjak, Snježana Galov, Ana Pathogens Article Fascioloidosis is a parasitic disease caused by a trematode Fascioloides magna. Since major histocompatibility complex (MHC) genes play an important role in the immune response, the aim of this study was to compare the potential differences in MHC class II SLA-DRB1 exon 2 genes between wild boar populations from infected (cases) and non-infected areas (controls). During the winter of 2021, a total of 136 wild boar tissue samples were collected, 39 cases and 97 controls. DNA was extracted and sequenced using the Illumina platform. Differences in distributions of allele combinations were calculated using the Chi-Square test for homogeneity and between proportions using the large-sample test and Fisher–Irwin test. Analysis revealed 19 previously described swine leucocyte antigen (SLA) alleles. The number of polymorphic sites was 79 (29.6%), with 99 mutations in total. Nucleotide diversity π was estimated at 0.11. Proportions of the alleles SLA-DRB1*12:05 (p = 0.0008379) and SLA-DRB1*0101 (p = 0.0002825) were statistically significantly higher in controls, and proportions of the SLA-DRB1*0602 (p = 0.006059) and SLA-DRB1*0901 (p = 0.0006601) in cases. Alleles SLA-DRB1*04:09, SLA-DRB1*0501, SLA-DRB1*11:09, and SLA-DRB1*1301 were detected only in cases, while SLA-DRB1*0404, SLA-DRB1*0701, SLA-DRB1*02:10, and SLA-DRB1*04:08 were present only in controls. We did not confirm the existence of specific alleles that could be linked to F. magna infection. Detected high variability of the MHC class II SLA-DRB1 exon 2 genes indicate high resistance potential against various pathogens. MDPI 2022-11-16 /pmc/articles/PMC9692795/ /pubmed/36422610 http://dx.doi.org/10.3390/pathogens11111359 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Konjević, Dean Erman, Vlatka Bujanić, Miljenko Svetličić, Ida Arbanasić, Haidi Lubura Strunjak, Snježana Galov, Ana Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes |
title | Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes |
title_full | Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes |
title_fullStr | Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes |
title_full_unstemmed | Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes |
title_short | Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes |
title_sort | wild boar (sus scrofa)—fascioloides magna interaction from the perspective of the mhc genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692795/ https://www.ncbi.nlm.nih.gov/pubmed/36422610 http://dx.doi.org/10.3390/pathogens11111359 |
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