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Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism

Exposure to bisphenol A (BPA) is unavoidable and it has far-reaching negative effects on living systems. This study aimed to explore the toxic effects of BPA in an experimental animal model through a metabolomics approach that is useful in measuring small molecule perturbations. Beside this, we also...

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Autores principales: Alvi, Maria, Rehman, Kanwal, Akash, Muhammad Sajid Hamid, Yaqoob, Azka, Shoaib, Syed Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692868/
https://www.ncbi.nlm.nih.gov/pubmed/36432690
http://dx.doi.org/10.3390/pharmaceutics14112496
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author Alvi, Maria
Rehman, Kanwal
Akash, Muhammad Sajid Hamid
Yaqoob, Azka
Shoaib, Syed Muhammad
author_facet Alvi, Maria
Rehman, Kanwal
Akash, Muhammad Sajid Hamid
Yaqoob, Azka
Shoaib, Syed Muhammad
author_sort Alvi, Maria
collection PubMed
description Exposure to bisphenol A (BPA) is unavoidable and it has far-reaching negative effects on living systems. This study aimed to explore the toxic effects of BPA in an experimental animal model through a metabolomics approach that is useful in measuring small molecule perturbations. Beside this, we also examined the ameliorative effects of resveratrol (RSV) against BPA-induced disturbances in experimental mice. This study was conducted for 28 days, and the results showed that BPA indeed induced an impairment in amino acid metabolism, taking place in the mitochondria by significantly (p < 0.05) decreasing the levels of certain amino acids, i.e., taurine, threonine, asparagine, leucine, norleucine, and glutamic acid in the mice plasma. However, the administration of RSV did prove effective against the BPA-induced intoxication and significantly (p < 0.05) restored the level of free amino acids. Lipid metabolites, L-carnitine, sphinganine, phytosphingosine, and lysophosphatidylcholine were also determined in the mice serum. A significant (p < 0.05) decline in glutathione peroxidase (GPx), superoxide dismutase (SOD,) glutathione, and catalase levels and an elevation in malondialdehyde level in the BPA group confirmed the generation of oxidative stress and lipid peroxidation in experimental mice exposed to BPA. The expression of Carnitine palmitoyltransferase I (CPT-I), carnitine palmitoyltransferase II (CPT-II), lecithin–cholesterol acyltransferase (LCAT), carnitine O-octanoyltransferase (CROT), carnitine-acylcarnitine translocase (CACT), and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) genes was significantly upregulated in the liver tissue homogenates of experimental mice exposed to BPA, although RSV regulated the expression of these genes when compared with BPA treated experimental mice. CPT-I, CPT-II, and CACT genes are located in the mitochondria and are involved in the metabolism and transportation of carnitine. Hence, this study confirms that BPA exposure induced oxidative stress, upregulated gene expression, and impaired lipid and amino acid metabolism in experimental mice.
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spelling pubmed-96928682022-11-26 Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism Alvi, Maria Rehman, Kanwal Akash, Muhammad Sajid Hamid Yaqoob, Azka Shoaib, Syed Muhammad Pharmaceutics Article Exposure to bisphenol A (BPA) is unavoidable and it has far-reaching negative effects on living systems. This study aimed to explore the toxic effects of BPA in an experimental animal model through a metabolomics approach that is useful in measuring small molecule perturbations. Beside this, we also examined the ameliorative effects of resveratrol (RSV) against BPA-induced disturbances in experimental mice. This study was conducted for 28 days, and the results showed that BPA indeed induced an impairment in amino acid metabolism, taking place in the mitochondria by significantly (p < 0.05) decreasing the levels of certain amino acids, i.e., taurine, threonine, asparagine, leucine, norleucine, and glutamic acid in the mice plasma. However, the administration of RSV did prove effective against the BPA-induced intoxication and significantly (p < 0.05) restored the level of free amino acids. Lipid metabolites, L-carnitine, sphinganine, phytosphingosine, and lysophosphatidylcholine were also determined in the mice serum. A significant (p < 0.05) decline in glutathione peroxidase (GPx), superoxide dismutase (SOD,) glutathione, and catalase levels and an elevation in malondialdehyde level in the BPA group confirmed the generation of oxidative stress and lipid peroxidation in experimental mice exposed to BPA. The expression of Carnitine palmitoyltransferase I (CPT-I), carnitine palmitoyltransferase II (CPT-II), lecithin–cholesterol acyltransferase (LCAT), carnitine O-octanoyltransferase (CROT), carnitine-acylcarnitine translocase (CACT), and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) genes was significantly upregulated in the liver tissue homogenates of experimental mice exposed to BPA, although RSV regulated the expression of these genes when compared with BPA treated experimental mice. CPT-I, CPT-II, and CACT genes are located in the mitochondria and are involved in the metabolism and transportation of carnitine. Hence, this study confirms that BPA exposure induced oxidative stress, upregulated gene expression, and impaired lipid and amino acid metabolism in experimental mice. MDPI 2022-11-17 /pmc/articles/PMC9692868/ /pubmed/36432690 http://dx.doi.org/10.3390/pharmaceutics14112496 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alvi, Maria
Rehman, Kanwal
Akash, Muhammad Sajid Hamid
Yaqoob, Azka
Shoaib, Syed Muhammad
Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism
title Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism
title_full Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism
title_fullStr Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism
title_full_unstemmed Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism
title_short Determination of Metabolomics Profiling in BPA-Induced Impaired Metabolism
title_sort determination of metabolomics profiling in bpa-induced impaired metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692868/
https://www.ncbi.nlm.nih.gov/pubmed/36432690
http://dx.doi.org/10.3390/pharmaceutics14112496
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