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Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy

Acute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involve...

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Autores principales: Rizzo-Valente, Vanessa S., Fusco, Maria A., Cruz, Renata M. M. L., Santos, Rachel A., Silva, Lucas S., Escaleira, Roberta C., Schulz, Daniel F., Barroso, Shana P. C., Miranda, Bruno L., Santos, Daniela Z., Gregório, Marcelo L., Guerra, Rodrigo J. A., Pavão, Mauro S. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693086/
https://www.ncbi.nlm.nih.gov/pubmed/36354999
http://dx.doi.org/10.3390/md20110676
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author Rizzo-Valente, Vanessa S.
Fusco, Maria A.
Cruz, Renata M. M. L.
Santos, Rachel A.
Silva, Lucas S.
Escaleira, Roberta C.
Schulz, Daniel F.
Barroso, Shana P. C.
Miranda, Bruno L.
Santos, Daniela Z.
Gregório, Marcelo L.
Guerra, Rodrigo J. A.
Pavão, Mauro S. G.
author_facet Rizzo-Valente, Vanessa S.
Fusco, Maria A.
Cruz, Renata M. M. L.
Santos, Rachel A.
Silva, Lucas S.
Escaleira, Roberta C.
Schulz, Daniel F.
Barroso, Shana P. C.
Miranda, Bruno L.
Santos, Daniela Z.
Gregório, Marcelo L.
Guerra, Rodrigo J. A.
Pavão, Mauro S. G.
author_sort Rizzo-Valente, Vanessa S.
collection PubMed
description Acute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involved in the wound healing properties of the glycosaminoglycan dermatan sulfate (DS), obtained from ascidian Styela plicata, a marine invertebrate, which in preliminary work from our group showed no toxicity and promoted a remarkable fibroblast proliferation and migration. In this study, 2,4-DS (50 µg/mL)-treated and control groups had the relative gene expression of 84 genes participating in the healing pathway evaluated. The results showed that 57% of the genes were overexpressed during treatment, 16% were underexpressed, and 9.52% were not detected. In silico analysis of metabolic interactions exhibited overexpression of genes related to: extracellular matrix organization, hemostasis, secretion of inflammatory mediators, and regulation of insulin-like growth factor transport and uptake. Furthermore, in C57BL/6 mice subjected to experimental wounds treated with 0.25% 2,4-DS, the histological parameters demonstrated a great capacity for vascular recovery. Additionally, this study confirmed that DS is a potent inducer of wound-healing cellular pathways and a promoter of neovascularization, being a natural ally in the tissue regeneration strategy.
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spelling pubmed-96930862022-11-26 Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy Rizzo-Valente, Vanessa S. Fusco, Maria A. Cruz, Renata M. M. L. Santos, Rachel A. Silva, Lucas S. Escaleira, Roberta C. Schulz, Daniel F. Barroso, Shana P. C. Miranda, Bruno L. Santos, Daniela Z. Gregório, Marcelo L. Guerra, Rodrigo J. A. Pavão, Mauro S. G. Mar Drugs Article Acute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involved in the wound healing properties of the glycosaminoglycan dermatan sulfate (DS), obtained from ascidian Styela plicata, a marine invertebrate, which in preliminary work from our group showed no toxicity and promoted a remarkable fibroblast proliferation and migration. In this study, 2,4-DS (50 µg/mL)-treated and control groups had the relative gene expression of 84 genes participating in the healing pathway evaluated. The results showed that 57% of the genes were overexpressed during treatment, 16% were underexpressed, and 9.52% were not detected. In silico analysis of metabolic interactions exhibited overexpression of genes related to: extracellular matrix organization, hemostasis, secretion of inflammatory mediators, and regulation of insulin-like growth factor transport and uptake. Furthermore, in C57BL/6 mice subjected to experimental wounds treated with 0.25% 2,4-DS, the histological parameters demonstrated a great capacity for vascular recovery. Additionally, this study confirmed that DS is a potent inducer of wound-healing cellular pathways and a promoter of neovascularization, being a natural ally in the tissue regeneration strategy. MDPI 2022-10-28 /pmc/articles/PMC9693086/ /pubmed/36354999 http://dx.doi.org/10.3390/md20110676 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rizzo-Valente, Vanessa S.
Fusco, Maria A.
Cruz, Renata M. M. L.
Santos, Rachel A.
Silva, Lucas S.
Escaleira, Roberta C.
Schulz, Daniel F.
Barroso, Shana P. C.
Miranda, Bruno L.
Santos, Daniela Z.
Gregório, Marcelo L.
Guerra, Rodrigo J. A.
Pavão, Mauro S. G.
Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
title Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
title_full Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
title_fullStr Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
title_full_unstemmed Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
title_short Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy
title_sort effects of dermatan sulfate from marine invertebrate styela plicata in the wound healing pathway: a natural resource applied to regenerative therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693086/
https://www.ncbi.nlm.nih.gov/pubmed/36354999
http://dx.doi.org/10.3390/md20110676
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