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Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol
Vaccination against SARS-CoV-2 and other viral infections requires safe, effective, and inexpensive vaccines that can be rapidly developed. DNA vaccines are candidates that meet these criteria, but one of their drawbacks is their relatively weak immunogenicity. Electroporation (EP) is an effective w...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693113/ https://www.ncbi.nlm.nih.gov/pubmed/36365078 http://dx.doi.org/10.3390/pharmaceutics14112259 |
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author | Kisakov, Denis Nikolaevich Kisakova, Lyubov Alexandrovna Borgoyakova, Maria Borisovna Starostina, Ekaterina Vladimirovna Taranov, Oleg Svyatoslavovich Ivleva, Elena Konstantinovna Pyankov, Oleg Viktorovich Zaykovskaya, Anna Vladimirovna Shcherbakov, Dmitry Nikolaevich Rudometov, Andrey Pavlovich Rudometova, Nadezda Borisovna Volkova, Natalia Vyacheslavovna Gureev, Vadim Nikolaevich Ilyichev, Alexander Alexeyevich Karpenko, Larisa Ivanovna |
author_facet | Kisakov, Denis Nikolaevich Kisakova, Lyubov Alexandrovna Borgoyakova, Maria Borisovna Starostina, Ekaterina Vladimirovna Taranov, Oleg Svyatoslavovich Ivleva, Elena Konstantinovna Pyankov, Oleg Viktorovich Zaykovskaya, Anna Vladimirovna Shcherbakov, Dmitry Nikolaevich Rudometov, Andrey Pavlovich Rudometova, Nadezda Borisovna Volkova, Natalia Vyacheslavovna Gureev, Vadim Nikolaevich Ilyichev, Alexander Alexeyevich Karpenko, Larisa Ivanovna |
author_sort | Kisakov, Denis Nikolaevich |
collection | PubMed |
description | Vaccination against SARS-CoV-2 and other viral infections requires safe, effective, and inexpensive vaccines that can be rapidly developed. DNA vaccines are candidates that meet these criteria, but one of their drawbacks is their relatively weak immunogenicity. Electroporation (EP) is an effective way to enhance the immunogenicity of DNA vaccines, but because of the different configurations of the devices that are used for EP, it is necessary to carefully select the conditions of the procedure, including characteristics such as voltage, current strength, number of pulses, etc. In this study, we determined the optimal parameters for delivery DNA vaccine by electroporation using the BEX CO device. BALB/c mice were used as a model. Plasmid DNA phMGFP was intramuscular (I/M) injected into the quadriceps muscle of the left hind leg of animals using insulin syringes, followed by EP. As a result of the experiments, the following EP parameters were determined: direct and reverse polarity rectangular DC current in three pulses, 12 V voltage for 30 ms and 950 ms intervals, with a current limit of 45 mA. The selected protocol induced a low level of injury and provided a high level of GFP expression. The chosen protocol was used to evaluate the immunogenicity of the DNA vaccine encoding the receptor-binding domain (RBD) of the SARS-CoV-2 protein (pVAXrbd) injected by EP. It was shown that the delivery of pVAXrbd via EP significantly enhanced both specific humoral and cellular immune responses compared to the intramuscular injection of the DNA vaccine. |
format | Online Article Text |
id | pubmed-9693113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96931132022-11-26 Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol Kisakov, Denis Nikolaevich Kisakova, Lyubov Alexandrovna Borgoyakova, Maria Borisovna Starostina, Ekaterina Vladimirovna Taranov, Oleg Svyatoslavovich Ivleva, Elena Konstantinovna Pyankov, Oleg Viktorovich Zaykovskaya, Anna Vladimirovna Shcherbakov, Dmitry Nikolaevich Rudometov, Andrey Pavlovich Rudometova, Nadezda Borisovna Volkova, Natalia Vyacheslavovna Gureev, Vadim Nikolaevich Ilyichev, Alexander Alexeyevich Karpenko, Larisa Ivanovna Pharmaceutics Article Vaccination against SARS-CoV-2 and other viral infections requires safe, effective, and inexpensive vaccines that can be rapidly developed. DNA vaccines are candidates that meet these criteria, but one of their drawbacks is their relatively weak immunogenicity. Electroporation (EP) is an effective way to enhance the immunogenicity of DNA vaccines, but because of the different configurations of the devices that are used for EP, it is necessary to carefully select the conditions of the procedure, including characteristics such as voltage, current strength, number of pulses, etc. In this study, we determined the optimal parameters for delivery DNA vaccine by electroporation using the BEX CO device. BALB/c mice were used as a model. Plasmid DNA phMGFP was intramuscular (I/M) injected into the quadriceps muscle of the left hind leg of animals using insulin syringes, followed by EP. As a result of the experiments, the following EP parameters were determined: direct and reverse polarity rectangular DC current in three pulses, 12 V voltage for 30 ms and 950 ms intervals, with a current limit of 45 mA. The selected protocol induced a low level of injury and provided a high level of GFP expression. The chosen protocol was used to evaluate the immunogenicity of the DNA vaccine encoding the receptor-binding domain (RBD) of the SARS-CoV-2 protein (pVAXrbd) injected by EP. It was shown that the delivery of pVAXrbd via EP significantly enhanced both specific humoral and cellular immune responses compared to the intramuscular injection of the DNA vaccine. MDPI 2022-10-22 /pmc/articles/PMC9693113/ /pubmed/36365078 http://dx.doi.org/10.3390/pharmaceutics14112259 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kisakov, Denis Nikolaevich Kisakova, Lyubov Alexandrovna Borgoyakova, Maria Borisovna Starostina, Ekaterina Vladimirovna Taranov, Oleg Svyatoslavovich Ivleva, Elena Konstantinovna Pyankov, Oleg Viktorovich Zaykovskaya, Anna Vladimirovna Shcherbakov, Dmitry Nikolaevich Rudometov, Andrey Pavlovich Rudometova, Nadezda Borisovna Volkova, Natalia Vyacheslavovna Gureev, Vadim Nikolaevich Ilyichev, Alexander Alexeyevich Karpenko, Larisa Ivanovna Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol |
title | Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol |
title_full | Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol |
title_fullStr | Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol |
title_full_unstemmed | Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol |
title_short | Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined Protocol |
title_sort | optimization of in vivo electroporation conditions and delivery of dna vaccine encoding sars-cov-2 rbd using the determined protocol |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693113/ https://www.ncbi.nlm.nih.gov/pubmed/36365078 http://dx.doi.org/10.3390/pharmaceutics14112259 |
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