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Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro

Yersinia ruckeri is an important fish pathogen causing enteric redmouth disease. Antibiotics have traditionally been used to control this pathogen, but concerns of antibiotic resistance have created a need for alternative interventions. Presently, chlorate and certain nitrocompounds were tested agai...

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Autores principales: Latham, Elizabeth A., Anderson, Robin C., Wottlin, Lauren R., Poole, Toni L., Crippen, Tawni L., Schlosser, Wayne D., Harvey, Roger B., Hume, Michael E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693156/
https://www.ncbi.nlm.nih.gov/pubmed/36422632
http://dx.doi.org/10.3390/pathogens11111381
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author Latham, Elizabeth A.
Anderson, Robin C.
Wottlin, Lauren R.
Poole, Toni L.
Crippen, Tawni L.
Schlosser, Wayne D.
Harvey, Roger B.
Hume, Michael E.
author_facet Latham, Elizabeth A.
Anderson, Robin C.
Wottlin, Lauren R.
Poole, Toni L.
Crippen, Tawni L.
Schlosser, Wayne D.
Harvey, Roger B.
Hume, Michael E.
author_sort Latham, Elizabeth A.
collection PubMed
description Yersinia ruckeri is an important fish pathogen causing enteric redmouth disease. Antibiotics have traditionally been used to control this pathogen, but concerns of antibiotic resistance have created a need for alternative interventions. Presently, chlorate and certain nitrocompounds were tested against Y. ruckeri as well as a related species within the genus, Y. aleksiciae, to assess the effects of these inhibitors. The results reveal that 9 mM chlorate had no inhibitory effect against Y. ruckeri, but inhibited growth rates and maximum optical densities of Y. aleksciciae by 20–25% from those of untreated controls (0.46 h(−1) and 0.29 maximum optical density, respectively). The results further reveal that 2-nitropropanol and 2-nitroethanol (9 mM) eliminated the growth of both Y. ruckeri and Y. aleksiciae during anaerobic or aerobic culture. Nitroethane, ethyl nitroacetate and ethyl-2-nitropropionate (9 mM) were less inhibitory when tested similarly. Results from a mixed culture of Y. ruckeri with fish tank microbes and of Y. aleksiciae with porcine fecal microbes reveal that the anti-Yersinia activity of the tested nitrocompounds was bactericidal, with 2-nitropropanol and 2-nitroethanol being more potent than the other tested nitrocompounds. The anti-Yersinia activity observed with these tested compounds warrants further study to elucidate the mechanisms of action and strategies for their practical application.
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spelling pubmed-96931562022-11-26 Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro Latham, Elizabeth A. Anderson, Robin C. Wottlin, Lauren R. Poole, Toni L. Crippen, Tawni L. Schlosser, Wayne D. Harvey, Roger B. Hume, Michael E. Pathogens Article Yersinia ruckeri is an important fish pathogen causing enteric redmouth disease. Antibiotics have traditionally been used to control this pathogen, but concerns of antibiotic resistance have created a need for alternative interventions. Presently, chlorate and certain nitrocompounds were tested against Y. ruckeri as well as a related species within the genus, Y. aleksiciae, to assess the effects of these inhibitors. The results reveal that 9 mM chlorate had no inhibitory effect against Y. ruckeri, but inhibited growth rates and maximum optical densities of Y. aleksciciae by 20–25% from those of untreated controls (0.46 h(−1) and 0.29 maximum optical density, respectively). The results further reveal that 2-nitropropanol and 2-nitroethanol (9 mM) eliminated the growth of both Y. ruckeri and Y. aleksiciae during anaerobic or aerobic culture. Nitroethane, ethyl nitroacetate and ethyl-2-nitropropionate (9 mM) were less inhibitory when tested similarly. Results from a mixed culture of Y. ruckeri with fish tank microbes and of Y. aleksiciae with porcine fecal microbes reveal that the anti-Yersinia activity of the tested nitrocompounds was bactericidal, with 2-nitropropanol and 2-nitroethanol being more potent than the other tested nitrocompounds. The anti-Yersinia activity observed with these tested compounds warrants further study to elucidate the mechanisms of action and strategies for their practical application. MDPI 2022-11-19 /pmc/articles/PMC9693156/ /pubmed/36422632 http://dx.doi.org/10.3390/pathogens11111381 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Latham, Elizabeth A.
Anderson, Robin C.
Wottlin, Lauren R.
Poole, Toni L.
Crippen, Tawni L.
Schlosser, Wayne D.
Harvey, Roger B.
Hume, Michael E.
Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
title Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
title_full Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
title_fullStr Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
title_full_unstemmed Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
title_short Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
title_sort inhibitory effect of select nitrocompounds and chlorate against yersinia ruckeri and yersinia aleksiciae in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693156/
https://www.ncbi.nlm.nih.gov/pubmed/36422632
http://dx.doi.org/10.3390/pathogens11111381
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