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Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging

In this study, sustainable, low-cost, and environmentally friendly biomass (Terminalia chebula) was employed as a precursor for the formation of nitrogen-doped carbon dots (N-CDs). The hydrothermally assisted Terminalia chebula fruit-derived N-CDs (TC-CDs) emitted different bright fluorescent colors...

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Autores principales: Atchudan, Raji, Perumal, Suguna, Edison, Thomas Nesakumar Jebakumar Immanuel, Sundramoorthy, Ashok K., Sangaraju, Sambasivam, Babu, Rajendran Suresh, Lee, Yong Rok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693165/
https://www.ncbi.nlm.nih.gov/pubmed/36432186
http://dx.doi.org/10.3390/molecules27228085
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author Atchudan, Raji
Perumal, Suguna
Edison, Thomas Nesakumar Jebakumar Immanuel
Sundramoorthy, Ashok K.
Sangaraju, Sambasivam
Babu, Rajendran Suresh
Lee, Yong Rok
author_facet Atchudan, Raji
Perumal, Suguna
Edison, Thomas Nesakumar Jebakumar Immanuel
Sundramoorthy, Ashok K.
Sangaraju, Sambasivam
Babu, Rajendran Suresh
Lee, Yong Rok
author_sort Atchudan, Raji
collection PubMed
description In this study, sustainable, low-cost, and environmentally friendly biomass (Terminalia chebula) was employed as a precursor for the formation of nitrogen-doped carbon dots (N-CDs). The hydrothermally assisted Terminalia chebula fruit-derived N-CDs (TC-CDs) emitted different bright fluorescent colors under various excitation wavelengths. The prepared TC-CDs showed a spherical morphology with a narrow size distribution and excellent water dispensability due to their abundant functionalities, such as oxygen- and nitrogen-bearing molecules on the surfaces of the TC-CDs. Additionally, these TC-CDs exhibited high photostability, good biocompatibility, very low toxicity, and excellent cell permeability against HCT-116 human colon carcinoma cells. The cell viability of HCT-116 human colon carcinoma cells in the presence of TC-CDs aqueous solution was calculated by MTT assay, and cell viability was higher than 95%, even at a higher concentration of 200 μg mL(−1) after 24 h incubation time. Finally, the uptake of TC-CDs by HCT-116 human colon carcinoma cells displayed distinguished blue, green, and red colors during in vitro imaging when excited by three filters with different wavelengths under a laser scanning confocal microscope. Thus, TC-CDs could be used as a potential candidate for various biomedical applications. Moreover, the conversion of low-cost/waste natural biomass into products of value promotes the sustainable development of the economy and human society.
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spelling pubmed-96931652022-11-26 Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging Atchudan, Raji Perumal, Suguna Edison, Thomas Nesakumar Jebakumar Immanuel Sundramoorthy, Ashok K. Sangaraju, Sambasivam Babu, Rajendran Suresh Lee, Yong Rok Molecules Article In this study, sustainable, low-cost, and environmentally friendly biomass (Terminalia chebula) was employed as a precursor for the formation of nitrogen-doped carbon dots (N-CDs). The hydrothermally assisted Terminalia chebula fruit-derived N-CDs (TC-CDs) emitted different bright fluorescent colors under various excitation wavelengths. The prepared TC-CDs showed a spherical morphology with a narrow size distribution and excellent water dispensability due to their abundant functionalities, such as oxygen- and nitrogen-bearing molecules on the surfaces of the TC-CDs. Additionally, these TC-CDs exhibited high photostability, good biocompatibility, very low toxicity, and excellent cell permeability against HCT-116 human colon carcinoma cells. The cell viability of HCT-116 human colon carcinoma cells in the presence of TC-CDs aqueous solution was calculated by MTT assay, and cell viability was higher than 95%, even at a higher concentration of 200 μg mL(−1) after 24 h incubation time. Finally, the uptake of TC-CDs by HCT-116 human colon carcinoma cells displayed distinguished blue, green, and red colors during in vitro imaging when excited by three filters with different wavelengths under a laser scanning confocal microscope. Thus, TC-CDs could be used as a potential candidate for various biomedical applications. Moreover, the conversion of low-cost/waste natural biomass into products of value promotes the sustainable development of the economy and human society. MDPI 2022-11-21 /pmc/articles/PMC9693165/ /pubmed/36432186 http://dx.doi.org/10.3390/molecules27228085 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Atchudan, Raji
Perumal, Suguna
Edison, Thomas Nesakumar Jebakumar Immanuel
Sundramoorthy, Ashok K.
Sangaraju, Sambasivam
Babu, Rajendran Suresh
Lee, Yong Rok
Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging
title Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging
title_full Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging
title_fullStr Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging
title_full_unstemmed Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging
title_short Sustainable Synthesis of Bright Fluorescent Nitrogen-Doped Carbon Dots from Terminalia chebula for In Vitro Imaging
title_sort sustainable synthesis of bright fluorescent nitrogen-doped carbon dots from terminalia chebula for in vitro imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693165/
https://www.ncbi.nlm.nih.gov/pubmed/36432186
http://dx.doi.org/10.3390/molecules27228085
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