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Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa
Onnia tomentosa is a widespread root rot pathogen frequently found in coniferous forests in North America. In this study, the potential medicinal properties of this wild polypore mushroom collected from north–central British Columbia, Canada, were investigated. The ethanol extract from O. tomentosa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693168/ https://www.ncbi.nlm.nih.gov/pubmed/36354930 http://dx.doi.org/10.3390/jof8111163 |
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author | Lee, Hooi Xian Li, Wai Ming Khatra, Jatinder Xia, Zhicheng Sannikov, Oleg Ling, Yun Zhu, Haoxuan Lee, Chow H. |
author_facet | Lee, Hooi Xian Li, Wai Ming Khatra, Jatinder Xia, Zhicheng Sannikov, Oleg Ling, Yun Zhu, Haoxuan Lee, Chow H. |
author_sort | Lee, Hooi Xian |
collection | PubMed |
description | Onnia tomentosa is a widespread root rot pathogen frequently found in coniferous forests in North America. In this study, the potential medicinal properties of this wild polypore mushroom collected from north–central British Columbia, Canada, were investigated. The ethanol extract from O. tomentosa was found to exhibit strong antiproliferative activity. Liquid–liquid extraction and bioactivity-guided fractionation, together with HPLC-MS/MS and 1D/2D NMR analyses of the ethanol extract of O. tomentosa, led to the identification of eight known linoleic oxygenated fatty acids (1.1–1.4 and 2–5), together with linoleic (6) and oleic acids (7). The autoxidation of linoleic acid upon isolation from a natural source and compound 5 as an autoxidation product of linoleic acid are reported here for the first time. GC-FID analysis of O. tomentosa, Fomitopsis officinalis, Echinodontium tinctorium, and Albatrellus flettii revealed linoleic, oleic, palmitic, and stearic acids as the major fatty acids. This study further showed that fatty acids were the major antiproliferative constituents in the ethanol extract from O. tomentosa. Linoleic acid and oleic acid had IC(50) values of 50.3 and 90.4 µM against human cervical cancer cells (HeLa), respectively. The results from this study have implications regarding the future exploration of O. tomentosa as a possible edible and/or medicinal mushroom. It is also recommended that necessary caution be taken when isolating unstable fatty acids from natural sources and in interpreting the results. |
format | Online Article Text |
id | pubmed-9693168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96931682022-11-26 Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa Lee, Hooi Xian Li, Wai Ming Khatra, Jatinder Xia, Zhicheng Sannikov, Oleg Ling, Yun Zhu, Haoxuan Lee, Chow H. J Fungi (Basel) Article Onnia tomentosa is a widespread root rot pathogen frequently found in coniferous forests in North America. In this study, the potential medicinal properties of this wild polypore mushroom collected from north–central British Columbia, Canada, were investigated. The ethanol extract from O. tomentosa was found to exhibit strong antiproliferative activity. Liquid–liquid extraction and bioactivity-guided fractionation, together with HPLC-MS/MS and 1D/2D NMR analyses of the ethanol extract of O. tomentosa, led to the identification of eight known linoleic oxygenated fatty acids (1.1–1.4 and 2–5), together with linoleic (6) and oleic acids (7). The autoxidation of linoleic acid upon isolation from a natural source and compound 5 as an autoxidation product of linoleic acid are reported here for the first time. GC-FID analysis of O. tomentosa, Fomitopsis officinalis, Echinodontium tinctorium, and Albatrellus flettii revealed linoleic, oleic, palmitic, and stearic acids as the major fatty acids. This study further showed that fatty acids were the major antiproliferative constituents in the ethanol extract from O. tomentosa. Linoleic acid and oleic acid had IC(50) values of 50.3 and 90.4 µM against human cervical cancer cells (HeLa), respectively. The results from this study have implications regarding the future exploration of O. tomentosa as a possible edible and/or medicinal mushroom. It is also recommended that necessary caution be taken when isolating unstable fatty acids from natural sources and in interpreting the results. MDPI 2022-11-03 /pmc/articles/PMC9693168/ /pubmed/36354930 http://dx.doi.org/10.3390/jof8111163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Hooi Xian Li, Wai Ming Khatra, Jatinder Xia, Zhicheng Sannikov, Oleg Ling, Yun Zhu, Haoxuan Lee, Chow H. Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa |
title | Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa |
title_full | Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa |
title_fullStr | Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa |
title_full_unstemmed | Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa |
title_short | Antiproliferative Fatty Acids Isolated from the Polypore Fungus Onnia tomentosa |
title_sort | antiproliferative fatty acids isolated from the polypore fungus onnia tomentosa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693168/ https://www.ncbi.nlm.nih.gov/pubmed/36354930 http://dx.doi.org/10.3390/jof8111163 |
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