Cargando…

The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis

Mycoplasma bovis (M. bovis) is an important pathogen affecting cattle, causing various diseases including pneumonia which mainly occurring in calves. Control of M. bovis infections is difficult due to the lack of commercial vaccines in most parts of the world and increasing trends of antimicrobial r...

Descripción completa

Detalles Bibliográficos
Autores principales: Dudek, Katarzyna, Szacawa, Ewelina, Wasiak, Magdalena, Bednarek, Dariusz, Reichert, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693237/
https://www.ncbi.nlm.nih.gov/pubmed/36365068
http://dx.doi.org/10.3390/pathogens11111317
_version_ 1784837488183869440
author Dudek, Katarzyna
Szacawa, Ewelina
Wasiak, Magdalena
Bednarek, Dariusz
Reichert, Michał
author_facet Dudek, Katarzyna
Szacawa, Ewelina
Wasiak, Magdalena
Bednarek, Dariusz
Reichert, Michał
author_sort Dudek, Katarzyna
collection PubMed
description Mycoplasma bovis (M. bovis) is an important pathogen affecting cattle, causing various diseases including pneumonia which mainly occurring in calves. Control of M. bovis infections is difficult due to the lack of commercial vaccines in most parts of the world and increasing trends of antimicrobial resistance in field isolates of the pathogen; therefore, it seems reasonable to look for new solutions for the prevention of the infection. Pegbovigrastim is a pegylated form of naturally occurring circulating cytokine in cattle that affects bovine leukocytes and some cell functions. Most studies on pegbovigrastim have focused on reducing the occurrence of mastitis and other diseases occurring during the periparturient period in cows, while this study attempts to use pegbovigrastim in the prevention of respiratory diseases in calves, which are largely caused by M. bovis. Based on previous observations on the immunostimulatory properties of pegbovigrastim in cattle, for the first time, the effect of its injection on the number and phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes in calves experimentally infected with M. bovis was investigated. Pegbovigrastim administration in the calves significantly stimulated an increase in peripheral blood granulocyte and monocyte counts and phagocytic activity of the cells, especially granulocytes, which was also generally expressed in the course of M. bovis infection. In response to pegbovigrastim administration, a general increase in the oxygen burst activity of the cells was observed. This effect was also shown despite ongoing infection with M. bovis which, taken together, may indicate a beneficial effect of pegbovigrastim injection on the immunity of the affected animals.
format Online
Article
Text
id pubmed-9693237
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96932372022-11-26 The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis Dudek, Katarzyna Szacawa, Ewelina Wasiak, Magdalena Bednarek, Dariusz Reichert, Michał Pathogens Article Mycoplasma bovis (M. bovis) is an important pathogen affecting cattle, causing various diseases including pneumonia which mainly occurring in calves. Control of M. bovis infections is difficult due to the lack of commercial vaccines in most parts of the world and increasing trends of antimicrobial resistance in field isolates of the pathogen; therefore, it seems reasonable to look for new solutions for the prevention of the infection. Pegbovigrastim is a pegylated form of naturally occurring circulating cytokine in cattle that affects bovine leukocytes and some cell functions. Most studies on pegbovigrastim have focused on reducing the occurrence of mastitis and other diseases occurring during the periparturient period in cows, while this study attempts to use pegbovigrastim in the prevention of respiratory diseases in calves, which are largely caused by M. bovis. Based on previous observations on the immunostimulatory properties of pegbovigrastim in cattle, for the first time, the effect of its injection on the number and phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes in calves experimentally infected with M. bovis was investigated. Pegbovigrastim administration in the calves significantly stimulated an increase in peripheral blood granulocyte and monocyte counts and phagocytic activity of the cells, especially granulocytes, which was also generally expressed in the course of M. bovis infection. In response to pegbovigrastim administration, a general increase in the oxygen burst activity of the cells was observed. This effect was also shown despite ongoing infection with M. bovis which, taken together, may indicate a beneficial effect of pegbovigrastim injection on the immunity of the affected animals. MDPI 2022-11-09 /pmc/articles/PMC9693237/ /pubmed/36365068 http://dx.doi.org/10.3390/pathogens11111317 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dudek, Katarzyna
Szacawa, Ewelina
Wasiak, Magdalena
Bednarek, Dariusz
Reichert, Michał
The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
title The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
title_full The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
title_fullStr The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
title_full_unstemmed The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
title_short The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
title_sort effect of pegbovigrastim injection on phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes in calves challenged with mycoplasma bovis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693237/
https://www.ncbi.nlm.nih.gov/pubmed/36365068
http://dx.doi.org/10.3390/pathogens11111317
work_keys_str_mv AT dudekkatarzyna theeffectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT szacawaewelina theeffectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT wasiakmagdalena theeffectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT bednarekdariusz theeffectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT reichertmichał theeffectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT dudekkatarzyna effectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT szacawaewelina effectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT wasiakmagdalena effectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT bednarekdariusz effectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis
AT reichertmichał effectofpegbovigrastiminjectiononphagocyticandoxidativeburstactivitiesofperipheralbloodgranulocytesandmonocytesincalveschallengedwithmycoplasmabovis