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Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune

Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmu...

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Autores principales: Timofeeva, Anna M., Sedykh, Sergey E., Ermakov, Evgeny A., Matveev, Andrey L., Odegova, Eva I., Sedykh, Tatiana A., Shcherbakov, Dmitry N., Merkuleva, Iuliia A., Volosnikova, Ekaterina A., Nesmeyanova, Valentina S., Tikunova, Nina V., Nevinsky, Georgy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693483/
https://www.ncbi.nlm.nih.gov/pubmed/36430159
http://dx.doi.org/10.3390/ijms232213681
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author Timofeeva, Anna M.
Sedykh, Sergey E.
Ermakov, Evgeny A.
Matveev, Andrey L.
Odegova, Eva I.
Sedykh, Tatiana A.
Shcherbakov, Dmitry N.
Merkuleva, Iuliia A.
Volosnikova, Ekaterina A.
Nesmeyanova, Valentina S.
Tikunova, Nina V.
Nevinsky, Georgy A.
author_facet Timofeeva, Anna M.
Sedykh, Sergey E.
Ermakov, Evgeny A.
Matveev, Andrey L.
Odegova, Eva I.
Sedykh, Tatiana A.
Shcherbakov, Dmitry N.
Merkuleva, Iuliia A.
Volosnikova, Ekaterina A.
Nesmeyanova, Valentina S.
Tikunova, Nina V.
Nevinsky, Georgy A.
author_sort Timofeeva, Anna M.
collection PubMed
description Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies.
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spelling pubmed-96934832022-11-26 Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune Timofeeva, Anna M. Sedykh, Sergey E. Ermakov, Evgeny A. Matveev, Andrey L. Odegova, Eva I. Sedykh, Tatiana A. Shcherbakov, Dmitry N. Merkuleva, Iuliia A. Volosnikova, Ekaterina A. Nesmeyanova, Valentina S. Tikunova, Nina V. Nevinsky, Georgy A. Int J Mol Sci Article Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies. MDPI 2022-11-08 /pmc/articles/PMC9693483/ /pubmed/36430159 http://dx.doi.org/10.3390/ijms232213681 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Timofeeva, Anna M.
Sedykh, Sergey E.
Ermakov, Evgeny A.
Matveev, Andrey L.
Odegova, Eva I.
Sedykh, Tatiana A.
Shcherbakov, Dmitry N.
Merkuleva, Iuliia A.
Volosnikova, Ekaterina A.
Nesmeyanova, Valentina S.
Tikunova, Nina V.
Nevinsky, Georgy A.
Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
title Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
title_full Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
title_fullStr Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
title_full_unstemmed Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
title_short Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
title_sort natural igg against s-protein and rbd of sars-cov-2 do not bind and hydrolyze dna and are not autoimmune
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693483/
https://www.ncbi.nlm.nih.gov/pubmed/36430159
http://dx.doi.org/10.3390/ijms232213681
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