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Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis

This article describes Neocosmospora keratoplastica as an etiological onychomycosis agent. Ex vivo studies were initially performed to demonstrate the ability of this species to grow and form a well-organized characteristic biofilm on sterilized healthy nails. Based on the history of excellent resul...

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Autores principales: Costa, Polyana de Souza, Cruz, Elton, Veiga, Flávia, Jarros, Isabelle Carrilho, Negri, Melyssa, Svidzinski, Terezinha Inez Estivalet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693509/
https://www.ncbi.nlm.nih.gov/pubmed/36422037
http://dx.doi.org/10.3390/jof8111216
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author Costa, Polyana de Souza
Cruz, Elton
Veiga, Flávia
Jarros, Isabelle Carrilho
Negri, Melyssa
Svidzinski, Terezinha Inez Estivalet
author_facet Costa, Polyana de Souza
Cruz, Elton
Veiga, Flávia
Jarros, Isabelle Carrilho
Negri, Melyssa
Svidzinski, Terezinha Inez Estivalet
author_sort Costa, Polyana de Souza
collection PubMed
description This article describes Neocosmospora keratoplastica as an etiological onychomycosis agent. Ex vivo studies were initially performed to demonstrate the ability of this species to grow and form a well-organized characteristic biofilm on sterilized healthy nails. Based on the history of excellent results, both for antifungal and antibiofilm, of propolis resin gum, we evaluated its activity using artificially formed biofilm. In vitro, the minimal biofilm eradication concentration of the propolis extract (PE) was 375 µg of total polyphenol content (TPC) per mL, while for the propolis gel (PG) it was 450 µg of TPC per mL. In biofilm exposed to the propolis products, a decrease in hyphae and conidia was evident, accompanied by a disorganization of the extracellular matrix. Additionally, this low concentration of PE was able to significantly reduce the number of colony-forming units and the metabolic activity. Furthermore, the treatment of a 15-year nail infection due to N. keratoplastica was carried out exclusively using a topical treatment with a gel containing propolis (30%) with a daily dosage. This treatment achieved complete remission of the onychomycosis in 12 months. It is important to point out that some inconveniences previously reported by other patients treated with propolis extract were eliminated, increasing adherence to treatment.
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spelling pubmed-96935092022-11-26 Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis Costa, Polyana de Souza Cruz, Elton Veiga, Flávia Jarros, Isabelle Carrilho Negri, Melyssa Svidzinski, Terezinha Inez Estivalet J Fungi (Basel) Article This article describes Neocosmospora keratoplastica as an etiological onychomycosis agent. Ex vivo studies were initially performed to demonstrate the ability of this species to grow and form a well-organized characteristic biofilm on sterilized healthy nails. Based on the history of excellent results, both for antifungal and antibiofilm, of propolis resin gum, we evaluated its activity using artificially formed biofilm. In vitro, the minimal biofilm eradication concentration of the propolis extract (PE) was 375 µg of total polyphenol content (TPC) per mL, while for the propolis gel (PG) it was 450 µg of TPC per mL. In biofilm exposed to the propolis products, a decrease in hyphae and conidia was evident, accompanied by a disorganization of the extracellular matrix. Additionally, this low concentration of PE was able to significantly reduce the number of colony-forming units and the metabolic activity. Furthermore, the treatment of a 15-year nail infection due to N. keratoplastica was carried out exclusively using a topical treatment with a gel containing propolis (30%) with a daily dosage. This treatment achieved complete remission of the onychomycosis in 12 months. It is important to point out that some inconveniences previously reported by other patients treated with propolis extract were eliminated, increasing adherence to treatment. MDPI 2022-11-17 /pmc/articles/PMC9693509/ /pubmed/36422037 http://dx.doi.org/10.3390/jof8111216 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Costa, Polyana de Souza
Cruz, Elton
Veiga, Flávia
Jarros, Isabelle Carrilho
Negri, Melyssa
Svidzinski, Terezinha Inez Estivalet
Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis
title Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis
title_full Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis
title_fullStr Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis
title_full_unstemmed Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis
title_short Efficacy of Propolis Gel on Mature Biofilm Formed by Neocosmospora keratoplastica Isolated from Onychomycosis
title_sort efficacy of propolis gel on mature biofilm formed by neocosmospora keratoplastica isolated from onychomycosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693509/
https://www.ncbi.nlm.nih.gov/pubmed/36422037
http://dx.doi.org/10.3390/jof8111216
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