Cargando…

Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line

Protein biosynthesis in mitochondria is tightly coupled with assembly of inner membrane complexes and therefore must be coordinated with cytosolic translation of the mRNAs corresponding to the subunits which are encoded in the nucleus. Molecular mechanisms underlying the regulation of mitochondrial...

Descripción completa

Detalles Bibliográficos
Autores principales: Baleva, Maria V., Chicherin, Ivan, Piunova, Uliana, Zgoda, Viktor, Patrushev, Maxim V., Levitskii, Sergey, Kamenski, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693627/
https://www.ncbi.nlm.nih.gov/pubmed/36430722
http://dx.doi.org/10.3390/ijms232214241
_version_ 1784837591922638848
author Baleva, Maria V.
Chicherin, Ivan
Piunova, Uliana
Zgoda, Viktor
Patrushev, Maxim V.
Levitskii, Sergey
Kamenski, Piotr
author_facet Baleva, Maria V.
Chicherin, Ivan
Piunova, Uliana
Zgoda, Viktor
Patrushev, Maxim V.
Levitskii, Sergey
Kamenski, Piotr
author_sort Baleva, Maria V.
collection PubMed
description Protein biosynthesis in mitochondria is tightly coupled with assembly of inner membrane complexes and therefore must be coordinated with cytosolic translation of the mRNAs corresponding to the subunits which are encoded in the nucleus. Molecular mechanisms underlying the regulation of mitochondrial translation remain unclear despite recent advances in structural biology. Until now, only one translational regulator of protein biosynthesis in mammalian mitochondria is known—protein TACO1, which regulates translation of COI mRNA. Here we describe the function of pentatricopeptide-containing protein PTCD2 as a translational regulator of another mitochondrially encoded subunit of cytochrome c oxidase—COIII in the HeLa cell line. Deletion of the PTCD2 gene leads to significant decrease in COIII translation efficiency and impairment in CIV activity. Additionally, we show that PTCD2 protein is partially co-sedimentates with associated mitochondrial ribosome and associates with mitochondrial ribosome proteins in pull-down assays. These data allow concluding that PTCD2 is a specific translational regulator of COIII which attracts the mRNA to the mitochondrial ribosome.
format Online
Article
Text
id pubmed-9693627
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96936272022-11-26 Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line Baleva, Maria V. Chicherin, Ivan Piunova, Uliana Zgoda, Viktor Patrushev, Maxim V. Levitskii, Sergey Kamenski, Piotr Int J Mol Sci Article Protein biosynthesis in mitochondria is tightly coupled with assembly of inner membrane complexes and therefore must be coordinated with cytosolic translation of the mRNAs corresponding to the subunits which are encoded in the nucleus. Molecular mechanisms underlying the regulation of mitochondrial translation remain unclear despite recent advances in structural biology. Until now, only one translational regulator of protein biosynthesis in mammalian mitochondria is known—protein TACO1, which regulates translation of COI mRNA. Here we describe the function of pentatricopeptide-containing protein PTCD2 as a translational regulator of another mitochondrially encoded subunit of cytochrome c oxidase—COIII in the HeLa cell line. Deletion of the PTCD2 gene leads to significant decrease in COIII translation efficiency and impairment in CIV activity. Additionally, we show that PTCD2 protein is partially co-sedimentates with associated mitochondrial ribosome and associates with mitochondrial ribosome proteins in pull-down assays. These data allow concluding that PTCD2 is a specific translational regulator of COIII which attracts the mRNA to the mitochondrial ribosome. MDPI 2022-11-17 /pmc/articles/PMC9693627/ /pubmed/36430722 http://dx.doi.org/10.3390/ijms232214241 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baleva, Maria V.
Chicherin, Ivan
Piunova, Uliana
Zgoda, Viktor
Patrushev, Maxim V.
Levitskii, Sergey
Kamenski, Piotr
Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line
title Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line
title_full Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line
title_fullStr Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line
title_full_unstemmed Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line
title_short Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line
title_sort pentatricopeptide protein ptcd2 regulates coiii translation in mitochondria of the hela cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693627/
https://www.ncbi.nlm.nih.gov/pubmed/36430722
http://dx.doi.org/10.3390/ijms232214241
work_keys_str_mv AT balevamariav pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline
AT chicherinivan pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline
AT piunovauliana pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline
AT zgodaviktor pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline
AT patrushevmaximv pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline
AT levitskiisergey pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline
AT kamenskipiotr pentatricopeptideproteinptcd2regulatescoiiitranslationinmitochondriaofthehelacellline