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A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish
Biogenic amines (BAs) are a family of nitrogen-bearing natural organic molecules with at least one primary amine, which play an important role in living organisms. Elevated concentration of BAs may cause neuron disorder, Parkinson's disease and many other diseases. Therefore, it is essential to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693732/ https://www.ncbi.nlm.nih.gov/pubmed/36505703 http://dx.doi.org/10.1039/d2ra06482k |
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author | Pei, Sheng-Lin Zhang, Jin Ge, Wanyun Liu, Chao Sheng, Ruilong Zeng, Lintao Pan, Ling-Hui |
author_facet | Pei, Sheng-Lin Zhang, Jin Ge, Wanyun Liu, Chao Sheng, Ruilong Zeng, Lintao Pan, Ling-Hui |
author_sort | Pei, Sheng-Lin |
collection | PubMed |
description | Biogenic amines (BAs) are a family of nitrogen-bearing natural organic molecules with at least one primary amine, which play an important role in living organisms. Elevated concentration of BAs may cause neuron disorder, Parkinson's disease and many other diseases. Therefore, it is essential to monitor BAs in living organisms. Herein, we reported a resorufin-based fluorescence probe for sensing of various BAs. Upon nucleophilic substitution reaction with BAs, the probe released resorufin, affording to strong fluorescence emission at 592 nm with rapid response (<8 min), good selectivity and a low detection limit (LOD = 0.47 μM). The probe has low cytotoxicity and good membrane permeability, and has been successfully used to visualize BAs in living cells and zebrafish with good performance. |
format | Online Article Text |
id | pubmed-9693732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-96937322022-12-08 A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish Pei, Sheng-Lin Zhang, Jin Ge, Wanyun Liu, Chao Sheng, Ruilong Zeng, Lintao Pan, Ling-Hui RSC Adv Chemistry Biogenic amines (BAs) are a family of nitrogen-bearing natural organic molecules with at least one primary amine, which play an important role in living organisms. Elevated concentration of BAs may cause neuron disorder, Parkinson's disease and many other diseases. Therefore, it is essential to monitor BAs in living organisms. Herein, we reported a resorufin-based fluorescence probe for sensing of various BAs. Upon nucleophilic substitution reaction with BAs, the probe released resorufin, affording to strong fluorescence emission at 592 nm with rapid response (<8 min), good selectivity and a low detection limit (LOD = 0.47 μM). The probe has low cytotoxicity and good membrane permeability, and has been successfully used to visualize BAs in living cells and zebrafish with good performance. The Royal Society of Chemistry 2022-11-25 /pmc/articles/PMC9693732/ /pubmed/36505703 http://dx.doi.org/10.1039/d2ra06482k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Pei, Sheng-Lin Zhang, Jin Ge, Wanyun Liu, Chao Sheng, Ruilong Zeng, Lintao Pan, Ling-Hui A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
title | A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
title_full | A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
title_fullStr | A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
title_full_unstemmed | A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
title_short | A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
title_sort | resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693732/ https://www.ncbi.nlm.nih.gov/pubmed/36505703 http://dx.doi.org/10.1039/d2ra06482k |
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