Cargando…

Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells

Cancer is one of the greatest threats to human health. Gastric cancer (GC) is the fifth most common malignant tumor in the world. Invasion and metastasis are the major difficulties in the treatment of GC. Herbal medicines and their extracts have a lengthy history of being used to treat tumors in Chi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Haibo, Luo, Yuanyuan, Hu, Yaqi, Feng, Xinyi, Feng, Jun, Chu, Zewen, Ou, Shiya, Dai, Xiaojun, Wang, Xiaoqing, Liu, Yanqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693919/
https://www.ncbi.nlm.nih.gov/pubmed/36432106
http://dx.doi.org/10.3390/molecules27228005
_version_ 1784837665337638912
author Wang, Haibo
Luo, Yuanyuan
Hu, Yaqi
Feng, Xinyi
Feng, Jun
Chu, Zewen
Ou, Shiya
Dai, Xiaojun
Wang, Xiaoqing
Liu, Yanqing
author_facet Wang, Haibo
Luo, Yuanyuan
Hu, Yaqi
Feng, Xinyi
Feng, Jun
Chu, Zewen
Ou, Shiya
Dai, Xiaojun
Wang, Xiaoqing
Liu, Yanqing
author_sort Wang, Haibo
collection PubMed
description Cancer is one of the greatest threats to human health. Gastric cancer (GC) is the fifth most common malignant tumor in the world. Invasion and metastasis are the major difficulties in the treatment of GC. Herbal medicines and their extracts have a lengthy history of being used to treat tumors in China. The anti-tumoral effects of the natural products derived from herbs have received a great deal of attention. Our previous studies have shown that the traditional Chinese herb Celastrus orbiculatus Thunb extract (COE) can inhibit the invasion and metastasis of GC cells, but the specific anti-cancer components of COE are still unclear. Dozens of natural products from COE have been isolated and identified by HPLC spectroscopy in our previous experiments. Triptonoterpene is one of the active ingredients in COE. In this study, we focused on revealing whether Triptonoterpene has an excellent anti-GC effect and can be used as an effective component of Celastrus orbiculatus Thunb in the treatment of tumors. We first observed that Triptonoterpene reduces GC cell proliferation through CCK-8 assays and colony formation experiments. The cell adhesion assays have shown that Triptonoterpene inhibits adhesion between cells and the cell matrix during tumor invasion. In addition, the cell migration assay has shown that Triptonoterpene inhibits the invasion and migration of GC cells. The high-connotation cell dynamic tracking experiment has also shown the same results. The effects of Triptonoterpene on epidermal mesenchymal transition (EMT)-related and matrix metalloproteinases (MMPs)-related proteins in gastric cancer cells were detected by Western blots. We found that Triptonoterpene could significantly inhibit the changes in EMT-related and invasion and metastasis-related proteins. Altogether, these results suggest that Triptonoterpene is capable of inhibiting the migration and invasion of GC cells. Triptonoterpene, as a natural product from Celastrus orbiculatus Thunb, has significant anti-gastric cancer effects, and is likely to be one of the major equivalent components of Celastrus orbiculatus Thunb.
format Online
Article
Text
id pubmed-9693919
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96939192022-11-26 Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells Wang, Haibo Luo, Yuanyuan Hu, Yaqi Feng, Xinyi Feng, Jun Chu, Zewen Ou, Shiya Dai, Xiaojun Wang, Xiaoqing Liu, Yanqing Molecules Article Cancer is one of the greatest threats to human health. Gastric cancer (GC) is the fifth most common malignant tumor in the world. Invasion and metastasis are the major difficulties in the treatment of GC. Herbal medicines and their extracts have a lengthy history of being used to treat tumors in China. The anti-tumoral effects of the natural products derived from herbs have received a great deal of attention. Our previous studies have shown that the traditional Chinese herb Celastrus orbiculatus Thunb extract (COE) can inhibit the invasion and metastasis of GC cells, but the specific anti-cancer components of COE are still unclear. Dozens of natural products from COE have been isolated and identified by HPLC spectroscopy in our previous experiments. Triptonoterpene is one of the active ingredients in COE. In this study, we focused on revealing whether Triptonoterpene has an excellent anti-GC effect and can be used as an effective component of Celastrus orbiculatus Thunb in the treatment of tumors. We first observed that Triptonoterpene reduces GC cell proliferation through CCK-8 assays and colony formation experiments. The cell adhesion assays have shown that Triptonoterpene inhibits adhesion between cells and the cell matrix during tumor invasion. In addition, the cell migration assay has shown that Triptonoterpene inhibits the invasion and migration of GC cells. The high-connotation cell dynamic tracking experiment has also shown the same results. The effects of Triptonoterpene on epidermal mesenchymal transition (EMT)-related and matrix metalloproteinases (MMPs)-related proteins in gastric cancer cells were detected by Western blots. We found that Triptonoterpene could significantly inhibit the changes in EMT-related and invasion and metastasis-related proteins. Altogether, these results suggest that Triptonoterpene is capable of inhibiting the migration and invasion of GC cells. Triptonoterpene, as a natural product from Celastrus orbiculatus Thunb, has significant anti-gastric cancer effects, and is likely to be one of the major equivalent components of Celastrus orbiculatus Thunb. MDPI 2022-11-18 /pmc/articles/PMC9693919/ /pubmed/36432106 http://dx.doi.org/10.3390/molecules27228005 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Haibo
Luo, Yuanyuan
Hu, Yaqi
Feng, Xinyi
Feng, Jun
Chu, Zewen
Ou, Shiya
Dai, Xiaojun
Wang, Xiaoqing
Liu, Yanqing
Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells
title Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells
title_full Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells
title_fullStr Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells
title_full_unstemmed Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells
title_short Triptonoterpene, a Natural Product from Celastrus orbiculatus Thunb, Has Biological Activity against the Metastasis of Gastric Cancer Cells
title_sort triptonoterpene, a natural product from celastrus orbiculatus thunb, has biological activity against the metastasis of gastric cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693919/
https://www.ncbi.nlm.nih.gov/pubmed/36432106
http://dx.doi.org/10.3390/molecules27228005
work_keys_str_mv AT wanghaibo triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT luoyuanyuan triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT huyaqi triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT fengxinyi triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT fengjun triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT chuzewen triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT oushiya triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT daixiaojun triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT wangxiaoqing triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells
AT liuyanqing triptonoterpeneanaturalproductfromcelastrusorbiculatusthunbhasbiologicalactivityagainstthemetastasisofgastriccancercells