Cargando…

Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients

Lupus nephritis (LN), a chronic inflammatory disease, is characterized by the substantial disruption of immune homeostasis. This study examines its effects on the T lymphocyte phenotype and, particularly, its senescence- and exhaustion-related immune alterations. T cell subpopulations were determine...

Descripción completa

Detalles Bibliográficos
Autores principales: Lioulios, Georgios, Mitsoglou, Zoi, Fylaktou, Asimina, Xochelli, Aliki, Christodoulou, Michalis, Stai, Stamatia, Moysidou, Eleni, Konstantouli, Afroditi, Nikolaidou, Vasiliki, Papagianni, Aikaterini, Stangou, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694088/
https://www.ncbi.nlm.nih.gov/pubmed/36430418
http://dx.doi.org/10.3390/ijms232213928
_version_ 1784837711468691456
author Lioulios, Georgios
Mitsoglou, Zoi
Fylaktou, Asimina
Xochelli, Aliki
Christodoulou, Michalis
Stai, Stamatia
Moysidou, Eleni
Konstantouli, Afroditi
Nikolaidou, Vasiliki
Papagianni, Aikaterini
Stangou, Maria
author_facet Lioulios, Georgios
Mitsoglou, Zoi
Fylaktou, Asimina
Xochelli, Aliki
Christodoulou, Michalis
Stai, Stamatia
Moysidou, Eleni
Konstantouli, Afroditi
Nikolaidou, Vasiliki
Papagianni, Aikaterini
Stangou, Maria
author_sort Lioulios, Georgios
collection PubMed
description Lupus nephritis (LN), a chronic inflammatory disease, is characterized by the substantial disruption of immune homeostasis. This study examines its effects on the T lymphocyte phenotype and, particularly, its senescence- and exhaustion-related immune alterations. T cell subpopulations were determined with flow cytometry in 30 LN patients and 20 healthy controls (HCs), according to the expression of senescence- (CD45RA, CCR7, CD31, CD28, CD57), and exhaustion- (PD1) related markers. The immune phenotype was associated with disease activity and renal histology. LN patients were characterized by pronounced lymphopenia, mainly affecting the CD4 compartment, with a concurrent reduction in the naïve, central and effector memory subsets compared to the HCs. In the CD8 compartment, the naïve subsets were significantly lower than that of the HCs, but a shift in the T cells occurred towards the central memory population. CD4+PD1+ and CD8+PD1+ cells were increased in the LN patients compared to the HCs. However, in CD4 T cells, the increase was limited to CD45RA+, whereas in CD8 T cells, both CD45RA+ and CD45RA− subsets were affected. Disease activity was correlated with CD4+PD1+ and highly differentiated CD4+CD28-CD57+ cells. Histology was only associated with CD4 T cell disturbances, with stage IV presenting reduced naïve and increased senescent subsets. Exhausted T lymphocyte subpopulations predominate within LN patients, while the T cell phenotype varies depending on disease activity.
format Online
Article
Text
id pubmed-9694088
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96940882022-11-26 Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients Lioulios, Georgios Mitsoglou, Zoi Fylaktou, Asimina Xochelli, Aliki Christodoulou, Michalis Stai, Stamatia Moysidou, Eleni Konstantouli, Afroditi Nikolaidou, Vasiliki Papagianni, Aikaterini Stangou, Maria Int J Mol Sci Article Lupus nephritis (LN), a chronic inflammatory disease, is characterized by the substantial disruption of immune homeostasis. This study examines its effects on the T lymphocyte phenotype and, particularly, its senescence- and exhaustion-related immune alterations. T cell subpopulations were determined with flow cytometry in 30 LN patients and 20 healthy controls (HCs), according to the expression of senescence- (CD45RA, CCR7, CD31, CD28, CD57), and exhaustion- (PD1) related markers. The immune phenotype was associated with disease activity and renal histology. LN patients were characterized by pronounced lymphopenia, mainly affecting the CD4 compartment, with a concurrent reduction in the naïve, central and effector memory subsets compared to the HCs. In the CD8 compartment, the naïve subsets were significantly lower than that of the HCs, but a shift in the T cells occurred towards the central memory population. CD4+PD1+ and CD8+PD1+ cells were increased in the LN patients compared to the HCs. However, in CD4 T cells, the increase was limited to CD45RA+, whereas in CD8 T cells, both CD45RA+ and CD45RA− subsets were affected. Disease activity was correlated with CD4+PD1+ and highly differentiated CD4+CD28-CD57+ cells. Histology was only associated with CD4 T cell disturbances, with stage IV presenting reduced naïve and increased senescent subsets. Exhausted T lymphocyte subpopulations predominate within LN patients, while the T cell phenotype varies depending on disease activity. MDPI 2022-11-11 /pmc/articles/PMC9694088/ /pubmed/36430418 http://dx.doi.org/10.3390/ijms232213928 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lioulios, Georgios
Mitsoglou, Zoi
Fylaktou, Asimina
Xochelli, Aliki
Christodoulou, Michalis
Stai, Stamatia
Moysidou, Eleni
Konstantouli, Afroditi
Nikolaidou, Vasiliki
Papagianni, Aikaterini
Stangou, Maria
Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients
title Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients
title_full Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients
title_fullStr Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients
title_full_unstemmed Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients
title_short Exhausted but Not Senescent T Lymphocytes Predominate in Lupus Nephritis Patients
title_sort exhausted but not senescent t lymphocytes predominate in lupus nephritis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694088/
https://www.ncbi.nlm.nih.gov/pubmed/36430418
http://dx.doi.org/10.3390/ijms232213928
work_keys_str_mv AT liouliosgeorgios exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT mitsoglouzoi exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT fylaktouasimina exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT xochellialiki exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT christodouloumichalis exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT staistamatia exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT moysidoueleni exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT konstantouliafroditi exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT nikolaidouvasiliki exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT papagianniaikaterini exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients
AT stangoumaria exhaustedbutnotsenescenttlymphocytespredominateinlupusnephritispatients