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Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats

Choline is an essential nutrient with many roles in brain development and function. Supplementation of choline in early development can have long-lasting benefits. Our experiments aimed to determine the efficacy of choline supplementation in a postnatal day (PND) 10 rat model of neonatal hypoxia isc...

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Autores principales: Adeyemo, Tayo, Jaiyesimi, Ayodele, Bumgardner, Jill G., Lohr, Charity, Banerjee, Aditi, McKenna, Mary C., Waddell, Jaylyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694200/
https://www.ncbi.nlm.nih.gov/pubmed/36430459
http://dx.doi.org/10.3390/ijms232213983
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author Adeyemo, Tayo
Jaiyesimi, Ayodele
Bumgardner, Jill G.
Lohr, Charity
Banerjee, Aditi
McKenna, Mary C.
Waddell, Jaylyn
author_facet Adeyemo, Tayo
Jaiyesimi, Ayodele
Bumgardner, Jill G.
Lohr, Charity
Banerjee, Aditi
McKenna, Mary C.
Waddell, Jaylyn
author_sort Adeyemo, Tayo
collection PubMed
description Choline is an essential nutrient with many roles in brain development and function. Supplementation of choline in early development can have long-lasting benefits. Our experiments aimed to determine the efficacy of choline supplementation in a postnatal day (PND) 10 rat model of neonatal hypoxia ischemia (HI) at term using both male and female rat pups. Choline (100 mg/kg) or saline administration was initiated the day after birth and given daily for 10 or 14 consecutive days. We determined choline’s effects on neurite outgrowth of sex-specific cultured cerebellar granule cells after HI with and without choline. The magnitude of tissue loss in the cerebrum was determined at 72 h after HI and in adult rats. The efficacy of choline supplementation in improving motor ability and learning, tested using eyeblink conditioning, were assessed in young adult male and female rats. Overall, we find that choline improves neurite outgrowth, short-term histological measures and learning ability in males. Surprisingly, choline did not benefit females, and appears to exacerbate HI-induced changes.
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spelling pubmed-96942002022-11-26 Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats Adeyemo, Tayo Jaiyesimi, Ayodele Bumgardner, Jill G. Lohr, Charity Banerjee, Aditi McKenna, Mary C. Waddell, Jaylyn Int J Mol Sci Article Choline is an essential nutrient with many roles in brain development and function. Supplementation of choline in early development can have long-lasting benefits. Our experiments aimed to determine the efficacy of choline supplementation in a postnatal day (PND) 10 rat model of neonatal hypoxia ischemia (HI) at term using both male and female rat pups. Choline (100 mg/kg) or saline administration was initiated the day after birth and given daily for 10 or 14 consecutive days. We determined choline’s effects on neurite outgrowth of sex-specific cultured cerebellar granule cells after HI with and without choline. The magnitude of tissue loss in the cerebrum was determined at 72 h after HI and in adult rats. The efficacy of choline supplementation in improving motor ability and learning, tested using eyeblink conditioning, were assessed in young adult male and female rats. Overall, we find that choline improves neurite outgrowth, short-term histological measures and learning ability in males. Surprisingly, choline did not benefit females, and appears to exacerbate HI-induced changes. MDPI 2022-11-12 /pmc/articles/PMC9694200/ /pubmed/36430459 http://dx.doi.org/10.3390/ijms232213983 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adeyemo, Tayo
Jaiyesimi, Ayodele
Bumgardner, Jill G.
Lohr, Charity
Banerjee, Aditi
McKenna, Mary C.
Waddell, Jaylyn
Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats
title Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats
title_full Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats
title_fullStr Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats
title_full_unstemmed Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats
title_short Choline Improves Neonatal Hypoxia-Ischemia Induced Changes in Male but Not Female Rats
title_sort choline improves neonatal hypoxia-ischemia induced changes in male but not female rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694200/
https://www.ncbi.nlm.nih.gov/pubmed/36430459
http://dx.doi.org/10.3390/ijms232213983
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